Electron microscopy of pathogenic yeasts Cryptococcus neoformans and Exophiala dermatitidis by high-pressure freezing

Yamaguchi, Masashi; Biswas, Sondip K.; Kita, Shigeo; Aikawa, Eizo; Takeo, Kanji

doi:10.1093/jmicro/51.1.21

Cited by 26 publications

(9 citation statements)

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“…The early work by Kopecka et al (1974) also demonstrates convincingly that the external layer of S. cerevisiae walls can be proteolytically removed without aVecting the inner wall and that the internal layer can be digested using -glucanases. Electron microscopic pictures of ascomycetous yeasts such as Candida tropicalis, Hanseniaspora osmophila, Kloeckera, Kluyveromyces polysporus, Schizosaccharomyces pombe, Torulopsis glabrata, and Exophila dermatitidis show a similar bi-layered cell wall (Baba and Osumi, 1987;Garrison, 1981;Yamaguchi et al, 2002). Collectively, these and many more additional data show that the walls of ascomycetous yeasts consist of an external protein layer strongly associated with and emanating from an inner polysaccharide layer.…”

Section: Morphological Features Of the Fungal Cell Wallmentioning

confidence: 84%

Features and functions of covalently linked proteins in fungal cell walls

Groot

Ram

Klis

2005

Fungal Genetics and Biology

268

250

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Section: Morphological Features Of the Fungal Cell Wallmentioning

confidence: 84%

Features and functions of covalently linked proteins in fungal cell walls

Groot

Ram

Klis

2005

Fungal Genetics and Biology

268

250

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“…In the research world the halo immediately stimulates questions about the nature of this striking material, its arrangement, and its biosynthesis. Beautiful scanning and thin-section EM (34, 136) and dramatic quick-freeze deep-etch images (119, 155) provide some hints, such as the intertwined fibers of capsular material emanating radially from close association with the cell wall (Figure 1), but many compelling questions remain.…”

Section: Cryptococcal Capsulementioning

confidence: 99%

How Sweet it is! Cell Wall Biogenesis and Polysaccharide Capsule Formation in Cryptococcus neoformans

Doering

2009

Annu. Rev. Microbiol.

199

229

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Cryptococcus neoformans is a pathogenic fungus responsible for severe opportunistic infections. The most prominent feature of this yeast is its elaborate polysaccharide capsule, a complex structure that is required for virulence. The capsule is intimately associated with the cell wall, which underlies the capsule and offers the organism strength and flexibility in potentially hostile environments. Both structures are primarily composed of polysaccharides, offering a glimpse of the tremendous variation inherent in natural carbohydrate structures and their multiple biological functions. The steps in cell wall and capsule biosynthesis and assembly pose fascinating questions of metabolism, enzymology, cell biology, and regulation; the answers have potential application to treatment of a deadly infection. This article reviews current knowledge of cryptococcal cell wall and capsule biosynthesis and outstanding questions for the future.

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“…They were haploid and used for a previous study 2 . Cells were cultured in 20 ml of YPG liquid medium 1% yeast extract, 1% polypeptone, and 1% glucose in a 100-ml Erlenmeyer flask at 24°C with reciprocal shaking at 150 rpm 5 . They grew exponentially and reached stationary phase at 22-26 hr.…”

Section: Strains Culture Conditions and Measurements Of Doubling Timentioning

confidence: 99%

Cytological Study of Cell Cycle of the Pathogenic Yeast Cryptococcus neoformans

Yamaguchi

Ohkusu

Biswas

et al. 2007

Nippon Ishinkin Gakkai Zasshi

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Cryptococcus neoformans is an opportunistic human pathogen belonging to basidiomycetous fungi and has unique properties in cell cycle progression. The purpose of this study was to measure the duration of the cell cycle in this yeast. Under standard liquid culture conditions 1% yeast extract, 1% polypeptone, and 1% glucose; 24°C; and 150 rpm , the doubling time of exponentially growing C. neoformans was 132 ± 16 min mean ± standard deviation , and the durations of the G1, S, G2, and M phases were about 71, 18, 25, and 18 min, respectively. DNA synthesis started before bud emergence, and finished by the time the size of the bud became 1/4 that of the mother cell. The doubling time of the daughter cells was about twice that of the mother cells. The spindle pole body was located on the outer nuclear envelope and showed a duplicated form from the G1 phase to the G2 phase. These data form a basis for further cell cycle study of C. neoformans.

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Electron microscopy of pathogenic yeasts Cryptococcus neoformans and Exophiala dermatitidis by high-pressure freezing

Cited by 26 publications

References 0 publications

Features and functions of covalently linked proteins in fungal cell walls

Features and functions of covalently linked proteins in fungal cell walls

How Sweet it is! Cell Wall Biogenesis and Polysaccharide Capsule Formation in Cryptococcus neoformans

Cytological Study of Cell Cycle of the Pathogenic Yeast Cryptococcus neoformans

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