Electron microscopy of previously identified cells and processes within the central nervous system

Ramon-Moliner, E.; Ferrari, J.

doi:10.1007/bf01098648

Cited by 16 publications

(1 citation statement)

References 13 publications

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“…The Golgi-impregnated blocks may be stored for as long as 2 months in 100% glycerol at 4°C or, better, at -20°C. The use of glycerol is convenient for maintaining the state of impregnation (Blackstad, 19751, and to optically clear the tissue for light microscopic observation (Ramon-Moliner and Ferrari, 1972).…”

Section: The Proceduresmentioning

confidence: 99%

Electron microscopy of Golgi‐impregnated interneurons: Notes on the intrinsic connectivity of the cerebral cortex

Fairén

Smith-Fernández

1992

Microscopy Res & Technique

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The Golgi‐electron microscope technique has opened new avenues to explore the synaptic organization of the brain. In this article, we shall discuss basic methodological principles necessary to analyze axonal arborizations with this combined technique. To illustrate the applications of the method, we shall review the forms and distribution of the synapses in which the axonal arborizations of local cortical interneurons engage. © 1992 Wiley‐Liss, Inc.

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Section: The Proceduresmentioning

confidence: 99%

Electron microscopy of Golgi‐impregnated interneurons: Notes on the intrinsic connectivity of the cerebral cortex

Fairén

Smith-Fernández

1992

Microscopy Res & Technique

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On the “selectivity” of the Golgi‐Cox method

Pasternak

Woolsey

1975

J of Comparative Neurology

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The randomness of the impregnation of layer IV cortical neurons by the Golgi-Cox method (Van der Loos, '56) has been assessed directly in Barrel C-1 of the mouse SmI. All Golgi-Cox impregnated neurons and unimpregnated neurons which were revealed with a Nissl counterstain were counted and measured in ten cerebral hemispheres cut tangential to the pia overlying the barrel field. The percentage of Golgi stained neurons varied considerably in different preparations from 0.73% to 2.26% with an average of 1.29%. The size distributions of both the Golgi impregnated and Nissl stained cells are similar but the difference of the means is statistically significant. However, if the means are equated there is no statistical difference in the two populations. When the Golgi precipitate is removed and the cells re-measured following Nissl staining there is a systematic reduction of the perikaryal cross-sectional area which is compatible with the differences in the means observed for the two populations as a whole. Finally, the frequency with which Golgi impregnated neurons are found in the barrel sides and hollows parallels the frequency with which Nissl stained neurons are observed in these two locations. We conclude that this variant of the Golgi method impregnates barrel neurons randomly. The value of this information for quantitative studies of cerebral cortex is discussed as is the potential of the system for elucidating some of the mechanisms responsible for Golgi impregnation.

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Smooth and sparsely‐spined stellate cells in the visual cortex of the rat: A study using a combined golgi‐electron microscope technique

Peters

Fairén

1978

J of Comparative Neurology

247

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The visual cortex of the rat was impregnated by the rapid Golgi procedure. From these preparations, smooth (spine-free) and sparselyspined multipolar stellate cells with well impregnated axons were selected and drawn in the light microscope using a camera lucida. Five suitably impregnated cells were then gold-toned and deimpregnated by removal of the silver chromate produced by the Golgi impregnation, so that they could be eKamined in the electron microscope to determine both their cytological features and their pre-and postsynaptic relationships. A sixth neuron was not deimpregnated, and was examined in the electron microscope only to evaluate the types of synapses formed by its axon terminals. Five of these cells had extensive local axonal plexuses and the sixth had a plexus which was less profuse.In the electron microscope the various portions of the deimpregnated neurons were readily identified by their content of fine gold particles, and it was found that their perikarya possessed a rather dark cytoplasm containing many ribosomes. Both symmetric and asymmetric synapses were present on the perikarya, and some of the perikarya had spines. The dendrites of the cells had relatively smooth contours and contained rather closely packed microtubules. The dendrites also had both symmetric and asymmetric synapses along their shafts, with the symmetric synapses being more frequent on the proximal portions of the dendrites.The axons of the neurons were unmyelinated and all of them formed symmetric synapses with their postsynaptic partners. The synapses occurred a t dilatations of the axons a t both en passant and terminal boutons. Neuronal elements identified as being postsynaptic to the axon terminals of the stellate cells included the perikarya and apical dendritic shafts of pyramidal neurons, the perikarya and dendritic shafts of other stellate cells, and an axon initial segment.Reasons are given for concluding that these multipolar, smooth and sparselyspined stellate cells are inhibitory in function, and their relationships with some other neuronal components of the rat visual cortex are considered.Nissl stained preparations of the cerebral cortex show the cell bodies of neurons in this part of the brain to be contained within horizontal layers, but it is apparent from Golgi preparations that none of these layers is composed of a homogeneous population of neurons. Instead, each layer contains the cell bodies of a variety of neuronal types, the dendrites of which often enter adjacent layers and frequently span several layers. Thus, there is an extensive intermixing of different forms of dendrites. The same is true of the axons of the cortical neurons, for not only do the intrinsic axons usually traverse several layers, but the neurons with axons which leave the cerebral cortex frequently give collaterals that also contribute to the neuropil. 129 130 ALAN PETERS AND ALFONSO FAIRENcrographs of the cerebral cortex difficult to analyse, not only in terms of identifying the profiles of dendrites and axons emanati...

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Electron microscopy of previously identified cells and processes within the central nervous system

Cited by 16 publications

References 13 publications

Electron microscopy of Golgi‐impregnated interneurons: Notes on the intrinsic connectivity of the cerebral cortex

Electron microscopy of Golgi‐impregnated interneurons: Notes on the intrinsic connectivity of the cerebral cortex

On the “selectivity” of the Golgi‐Cox method

Smooth and sparsely‐spined stellate cells in the visual cortex of the rat: A study using a combined golgi‐electron microscope technique

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