Electron microscopy of ribonucleoprotein particles from E. Coli

Protein Adsorption on Nano-scaled, Rippled TiO2 and Si Surfaces

“…A general understanding of the mechanisms involved in protein adsorption can be gained by investigating human plasma fibrinogen (HPF) on surfaces, because of its importance for the blood coagulation process and the fact that its structure is well known [1,7,8]. Most notably, HPF has an amphiphilic character that causes its hydrophobic and hydrophilic parts to be attracted to hydrophobic and hydrophilic surfaces, respectively, assuming different conformations.…”

An Electron Microscopic Study of Large Bacterial Polyribosomes

“…There have been many electron microscopic studies of these structures in tissue sections, but comparatively few studies have been reported on polysomes after they have been isolated from cells (21,19). Studies of protein synthesis are frequently carried out in Escherichia coli but, although electron microscopic studies have been done on ribosomes (6,8) and small polysomes (5), there have been no reports on the morphology of large polysomes of E. coli. In the course of studying the appearance of the inducible enzyme j3-galactosidase on the polysomes of E. coli, gentle methods of cell lysis were developed which made it possible for us to prepare what appeared to be largely undegraded polysomes (11).…”

The combination of the viscosity increment with the harmonic mean rotational relaxation time for determining the conformation of biological macromolecules in solution

“…This corresponds to a prolate ellipsoid of axial ratio 6.8 + 0.3, consistent with the estimates of the axial ratio derived from four other hydrodynamic parameters, three of which assume no particle swelling due to solvent association ( Table 2). The results from electronmicroscopic studies suggest, however, that the subfragments are nearly spherical (Hall & Slayter, 1959); as Mihalyi & Godfrey (1963) have previously stated, this difference is probably too large to be explained by drying effects alone. At least part of this difference can, however, possibly be ascribed to an apparent discrepancy between the viscosity data of their Fig.…”