Electron microscopy of the epiphyseal apparatus

Scott, Berenice; Pease, Daniel C.

doi:10.1002/ar.1091260405

Cited by 237 publications

(108 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Odontoclasts resorbing tooth are cells which have the common morphological features of osteoclasts resorbing bone: multinucleation, the presence of a ruffled border observed by TEM (Scott and Pease, 1956;Ten Care and Anderson, 1986;Marks and Popoff, 1988;Pierce et al, 1991). Although the cellular differentiation of osteoclasts and odontoclasts is well documented (Ejiri, 1983;Takahashi et al, 1988;Sahara et al, 1996;Domon et al, 1997Domon et al, , 1998, the fate of these cells, their apoptosis, has not been documented.…”

Section: Introductionmentioning

confidence: 99%

Apoptosis of odontoclasts under physiological root resorption of human deciduous teeth

et al. 2007

View full text Add to dashboard Cite

SummaryThis study was designed to establish apoptosis of odontoclasts under physiological root resorption of human deciduous teeth. Deciduous teeth were fixed, decalcified, and embedded in paraffin for immunohistochemical (IHC) observations and in Epon for transmission electron microscopy (TEM). Apoptotic cells were identified by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick end labeling (TUNEL), and then the tartrate-resistant acid phosphatase (TRAP) activity was determined on the same sections. Epon embedded specimens were sectioned serially into 0.5-µm semithin sections, and some of the sections were re-embedded into Epon, sectioned into 0.1-µm ultrathin sections, and then observed by TEM. By IHC, the nuclei of TRAP-positive odontoclasts on the dentine were generally TUNEL-negative. Around these odontoclasts, there were a few TRAP-positive structures with TUNEL-positive structures: A TRAP-positive structure with one TUNEL-positive nucleus, a TRAP-positive structure with one TUNEL-positive nucleus and one or two TUNEL-negative nuclei, and a TRAP-positive structure without any nucleus. By TEM, some odontoclasts showed nuclear fragments including compacted chromatin. The results suggest that, in apoptosis, odontoclasts fragment into variously sized cellular parts including three or fewer nuclei.3

show abstract

Section: Introductionmentioning

confidence: 99%

Apoptosis of odontoclasts under physiological root resorption of human deciduous teeth

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Cells were determined to be osteoclasts based on their fine structures (Fig. 3) and previously described features of osteoclasts in vitro and in vivo (SCOTT and PEASE, 1956;GONZALES and KARNOVSKY, 1961;DUDLEY and SPIRO, 1961;SCHENK et al, 1967;KALLIO et al, 1971;FETTER and CAPEN, 1971;LUCHT, 1972;BONUCCI, 1974;HOLTROP et al, 1974;LUK et al, 1974;SUZUKI, 1974;MILLER, 1978;WEZEMAN et al, 1979;Ko and BERNERD, 1981;WAKITA, 1986, 1989).…”

Section: Discussionmentioning

confidence: 99%

Studies of the clear zone of osteoclasts: Immunohistological aspects of its form and distribution.

Yoshida

Domon

Wakita

1989

Archives of Histology and Cytology

View full text Add to dashboard Cite

“…The theory of charge-transfer complexes has recently been discussed by Kosower21. (22) Stabilization of the ground state of a charge-transfer complex by a contribution from the charge-transferred form implies that, among other things, the complexing constant, K, might be related to the donor and acceptor properties of the components of the complex.…”

Section: Charge-transfer Complexes In Cyclopolymerizationmentioning

confidence: 99%

The role of charge-transfer complexes in cyclocopolymerization

Butler¹

1970

Pure and Applied Chemistry

View full text Add to dashboard Cite

ABSTRACT.Previous work from this laboratory has shown that certain 1 ,4-dienes which readily undergo cyclocopolymerization with certain alkenes also form chargetransfer complexes with the same alkenes. The results observed and the proposed cyciocopolymerization mechanism are consistent with participation of the charge-transfer complex as a distinct species in the copolymerization. This paper includes a discussion of an investigation to determine whether there was a dilution effect on the relative reactivities of the monomers in support of the charge-transfer participation concept, and whether the results of a suitable terpolymerization study would also support this postulate. In the system, divinyl ether-fumaronitrile, the maximum rate of copolymerization occurred at a monomer feed ratio of 1:2 and the composition of the copolymer was also 1:2 ata total monomer concentration of 3 moles/i. However, when the concentration was progressively lowered to 05 ml1, using the same monomer feed ratio, the fumaronitrile content of the copolymer decreased in a linear manner. In a series of terpolymerization experiments using the system, divinyl ethermaleic anhydride-acrylonitrile, it was shown that the divinyl ether-maleic anhydride ratio in the terpolymer was always greater than 1: 1 and had an upper limit of 1:2, regardless of the feed ratio of the termonomers. These results are consistent with the participation of the charge-transfer complex of divinyl ether and maleic anhydride in a copolymerization process with either maleic anhydride or acrylonitrile as the comonomer. Other systems which support these concepts are also presented. This paper emphasizes theoretical aspects of the problem rather than attempting to summarize the many examples of comonomer systems which have been found to participate in this interesting copolymerization.

show abstract

Electron microscopy of the epiphyseal apparatus

Cited by 237 publications

References 26 publications

Apoptosis of odontoclasts under physiological root resorption of human deciduous teeth

Apoptosis of odontoclasts under physiological root resorption of human deciduous teeth

Studies of the clear zone of osteoclasts: Immunohistological aspects of its form and distribution.

The role of charge-transfer complexes in cyclocopolymerization

Contact Info

Product

Resources

About