Electron microscopy studies on luteovirid transmission by aphids

Brault, Véronique; Herrbach, Étienne; Reinbold, Catherine

doi:10.1016/j.micron.2006.04.005

Cited by 88 publications

(84 citation statements)

References 53 publications

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“…This was not surprising considering that previous work had shown that virions of BYDV devoid of the RTP were able to cross the gut barrier, indicating that the CP possessed the determinants for mediating gut uptake (Chay et al, 1996). However, RTP can affect the efficiency of virus transport across the gut (Brault et al, 2000(Brault et al, , 2007Reinbold et al, 2001) and RTP can determine intestinal tropism when mediating acquisition (Brault et al, 2005).…”

Section: Aphid Transmission Of the Rtd Mutants Via Tissue Feeding Andmentioning

confidence: 99%

Small deletions in the potato leafroll virus readthrough protein affect particle morphology, aphid transmission, virus movement and accumulation

Peter

Liang

Palukaitis³

et al. 2008

Journal of General Virology

120

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Potato leafroll virus (PLRV) capsid comprises 180 coat protein (CP) subunits, with some percentage containing a readthrough domain (RTD) extension located on the particle's surface. The RTD N terminus is highly conserved in luteovirids and this study sought to identify biologically active sites within this region of the PLRV RTD. Fourteen three-amino-acid-deletion mutants were generated from a cloned infectious PLRV cDNA and delivered to plants by Agrobacterium inoculations. All mutant viruses accumulated locally in infiltrated tissues and expressed the readthrough protein (RTP) containing the CP and RTD sequences in plant tissues; however, when purified, only three mutant viruses incorporated the RTP into the virion. None of the mutant viruses were aphid transmissible, but the viruses persisted in aphids for a period sufficient to allow for virus transmission. Several mutant viruses were examined further for systemic infection in four host species. All mutant viruses, regardless of RTP incorporation, moved systemically in each host, although they accumulated at different rates in systemically infected tissues. The biological properties of the RTP are sensitive to modifications in both the RTD conserved and variable regions.

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Section: Aphid Transmission Of the Rtd Mutants Via Tissue Feeding Andmentioning

confidence: 99%

Small deletions in the potato leafroll virus readthrough protein affect particle morphology, aphid transmission, virus movement and accumulation

Peter

Liang

Palukaitis³

et al. 2008

Journal of General Virology

120

View full text Add to dashboard Cite

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“…Damage from S. avenae is both direct, through mechanical damage and injection of chemical substances in saliva, and indirect, through the effect of honeydew and pathogenic fungi. S. avenae also is a highly efficient vector of barley yellow dwarf virus (Brault et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Changes in Pigment Content of Triticale Genotypes Infested with Grain Aphid Sitobion Avenae (Fabricius) (Homoptera: Aphididae)

Wójcicka¹

2014

Acta Biologica Cracoviensia S. Botanica

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The effect of feeding by the grain aphid Sitobion avenae (Fabricius) (Homoptera: Aphididae) on chlorophyll, carotenoid and flavonoid content was studied in waxy and waxless triticale genotypes. On both sampling dates (5 and 10 days after infestation), seedlings of infested waxy and waxless plants had lower chlorophylls and carotenoids and higher flavonoids than in uninfested plants.K Ke ey y w wo or rd ds s: : Biotic stress, chlorophyll, carotenoid, flavonoid, Triticosecale, Sitobion avenae, waxes.

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“…Due to piercing-sucking mouthparts and their feeding behaviour, aphids are well suited for spreading viruses from one plant to another. Brault et al [2] studied the transport mechanism of luteovirids through aphids and they found that earlier, ingested virions are acquired in the food canal and passed to the haemolymph through the posterior midgut or hindgut or through both depending on the virus species. Virions then enter the accessory salivary glands and are subsequently released into plant hosts through the salivary duct.…”

Section: Discussionmentioning

confidence: 99%

Quantification of sugarcane yellow leaf virus in sugarcane following transmission through aphid vector, Melanaphis sacchari

Chinnaraja

Viswanathan

2015

VirusDis.

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Yellow leaf caused by Sugarcane yellow leaf virus (SCYLV) is a serious constraint to sugarcane production in India and currently the disease epidemics occur on many of the susceptible varieties under field conditions. Studies were conducted on the virus transmission by sugarcane aphid Melanaphis sacchari in sugarcane by inoculating virus-free meristem derived from micro-propagated plants of sugarcane cv Co 86032 with viruliferous aphids. Virus transmission was confirmed through RT-PCR assays and subsequently SCYLV population was established through RT-qPCR. A maximum of 22.3 9 10 3 , 3.16 9 10 6 and 4.78 9 10 6 copies of SCYLV-RNA targets were recorded in the plants after 7, 180 and 300 days, respectively. This study showed that the aphid species M. sacchari acts as an effective vector of SCYLV. The relative standard curve method in RT-qPCR efficiently detected the increment in SCYLV copy numbers in sugarcane following transmission through M. sacchari.

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Electron microscopy studies on luteovirid transmission by aphids

Cited by 88 publications

References 53 publications

Small deletions in the potato leafroll virus readthrough protein affect particle morphology, aphid transmission, virus movement and accumulation

Small deletions in the potato leafroll virus readthrough protein affect particle morphology, aphid transmission, virus movement and accumulation

Changes in Pigment Content of Triticale Genotypes Infested with Grain Aphid Sitobion Avenae (Fabricius) (Homoptera: Aphididae)

Quantification of sugarcane yellow leaf virus in sugarcane following transmission through aphid vector, Melanaphis sacchari

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