Electron Paramagnetic Resonance - A Powerful Tool of Medical Biochemistry in Discovering Mechanisms of Disease and Treatment Prospects

Spasojević, Ivan

doi:10.2478/v10011-010-0020-0

Cited by 15 publications

(12 citation statements)

References 124 publications

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“…The main function of erythrocytes is the transport of oxygen (O 2 ) and mediation of carbon dioxide (CO 2 ) production (13). As the red blood cell emerges from the bone marrow, it loses its nucleus, ribosomes, and mito chondria, and therefore the capacity for cell division, protein synthesis, and mitochondrial-based oxidative reactions (13,14). As a consequence of their physiologic role, erythrocytes are exposed to continuous oxidative stress.…”

Section: Antioxidative Enzymes In Erythrocytesmentioning

confidence: 99%

Complexity of free radical Metabolism in human Erythrocytes

Nikolić‐Kokić¹,

Blagojević²,

Spasić³

2010

Journal of Medical Biochemistry

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Kratak sadr`aj: Produkcija slobodnih radikala u eritrocitima uglavnom se odnosi na nastajanje superoksid anjon radikala (O 2 .-) putem autooksidacije oksihemoglobina u methemoglobin. Ljudski eritrociti izlo`eni su prooksidacionom delovanju vodonik-peroksida nastalog dismutacijom O 2 .-ili iz cirkulacije, kao i azot oksidu (NO) iz cirkulacije. Od di rektnih reakcija slobodnih radikala, reakcija O 2 .-i NO uz nastajanje peroksinitrita je reakcija sa primarno {tetnim posledicama po eritrocite. U eritrocitima se nalaze enzimi za{tite od oksidacionih o{te}enja, kao {to su superoksid dismu taza (SOD, EC 1.15.1.1), katalaza (CAT, EC 1.11.1.6), glutation peroksidaza (GSHPx, EC 1.11.1.9) i glutation reduktaza (GR, EC 1.6.4.2) kao i komponente male mo lekulske mase (glutation, vitamini E i C). Njihovim sadej stvom se kanali{u reakcije slobodnih radikala tako da di rektna o{te -}enja biomakromolekula budu {to manja. Me|u tim, kako nema de novo sinteze enzima u maturiranim eri trocitima, kapacitet ovih sistema je ograni~en, jer slobo d noradikalske vrste i direktno inhibiraju neke od enzima. Promene na enzimima i njihova inhibicija slobodnim radi kalima uti~u na kapacitet za{tite od oksidacionih o{te}enja i relativni udeo pojedinih komponenti u ukupnom antioksi da tivnom poten cijalu. To se mo`e pratiti i preko promena aktiv nosti pojedina~nih komponenti, ali i me |u sobnih odnosa izme|u komponenti antioksidativne odbrane diskrimina cio nim sta ti sti ~kim metodama, koje ukazuju na sveukupnost i kompleksnost odnosa antioksida tivnih kompo nenti u eritro citima i njihov sistemski zna~aj.

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Section: Antioxidative Enzymes In Erythrocytesmentioning

confidence: 99%

Complexity of free radical Metabolism in human Erythrocytes

Nikolić‐Kokić¹,

Blagojević²,

Spasić³

2010

Journal of Medical Biochemistry

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“…The main function of red blood cells is to transport oxygen and carbon dioxide. During erythropoiesis, erythrocytes lose nucleus, ribosomes, mitochondria, and thus the ability to cell division, protein synthesis and oxidative processes inherent in mitochondria [15]. Due to their physiological role, the red blood cells are permanently affected by endogenous oxidative stress: the auto-oxidation of oxyhemoglobin to methemoglobin generates superoxide radical (O 2…”

Section: T a B L E 1 Tumor Weight Tumor Inhibition Index And Concenmentioning

confidence: 99%

Changes in oxidative stress intensity in blood of tumor-bearing rats following different modes of administration of rhenium-platinum system

Shamelashvili¹,

Штеменко²,

Leus³

et al. 2016

Ukr.Biochem.J.

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“…However, these also have drawbacks (5,(24)(25)(26). Recently, emphasis has been placed on therapeutically useful essential oils from aromatic plants (6).…”

Section: Discussionmentioning

confidence: 99%

Vitex Agnus-Castus L. Essential Oil Increases Human Erythrocyte Membrane Fluidity

Ajdžanović¹,

Spasojević²,

Pantelic³

et al. 2012

Journal of Medical Biochemistry

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Summary: Erythrocyte membrane fluidity is related to their rheologic behavior, the dynamic quality of erythrocytes, which is tempted in hypertension and atherosclerosis. An increased risk of these and other cardiovascular diseases occurs in ageing women. Menopause-related conditions are often treated with hormone replacement therapy that may increase the risk of malignancies. Vitex agnus-castus L. essential oil contains various organic compounds (monoterpenes, sesquiterpenes and terpenoids), and is increasingly used as an alternative therapy for menopausal symptoms. These components of the oil may be incorporated into cell membranes, thereby changing the membrane fluidity. The aim of this study was to determine the effects of Vitex agnuscastus essential oil on human erythrocyte membrane fluidity at graded depths. We used Electron Paramagnetic Resonan ce spectroscopy and fatty acid spin probes (5-doxyl stearic acid and 12-doxyl stearic acid), whose spectra depend on membrane fluidity. After treatment with Vitex agnus-castus essential oil the erythrocytes had a significant (p=0.029) and reversible increase in membrane fluidity in the deeper hydrophobic membrane regions, with no change (p>0.05) in fluidity near the membrane's hydrophilic surface. These results document increased fluidity of the human erythrocyte membrane by Vitex agnus-castus essential oil, and this action may be useful in patients with menopause--related hypertension and other cardiovascular conditions. Keywords: erythrocytes, membrane fluidity, Vac essential oil, EPR Kratak sadr`aj: Fluidnost membrane eritrocita uslovljava njihove reolo{ke karakteristike, svojevrsne dinami~ke kva litete, izmenjene u hipertenziji i aterosklerozi. Rizik od navedenih i drugih kardiovaskularnih oboljenja se kod `ena po ve}ava sa sta renjem. Tegobe koje prate menopauzu se obi~no ubla`avaju supstitucionom hormonskom terapijom, koja mo`e pove}ati rizik od pojave malignih oboljenja. Esencijalno ulje biljke Vitex agnus-castus L. sadr`i razli~ita organska jedinjenja (monoterpene, seskviterpene i terpenoide) i svẽ e{}e se koristi kao alternativno terapijsko sredstvo za simptome me no pauze. Navedene komponente esencijalnog ulja mogu se ugraditi u }elijske membrane i menjati njihovu fluidnost. Cilj studije bio je da se utvrde efekti esencijalnog ulja biljke Vitex agnus-castus na fluidnost membrane eritrocita po njenoj du bini. Kori{}ena je spektroskopija elektronske paramagnetne rezonance i masnokiselinske spinske probe (5-dok sil stea rinska i 12-doksil stearinska kiselina), ~iji spektri za vise od fluidnosti membrane. Nakon tretmana eritrocita esencijal nim uljem biljke Vitex agnus-castus utvr|eno je zna~ajno (p=0,029) i reverzibilno pove}anje fluidnosti njihove membrane u dubljim, hidrofobnim regionima, dok u povr{inskim, hidrofilnim regionima eritrocitne membrane nisu utvr|ene zna~ajne promene (p>0,05). Ovi rezultati uka zuju na po ve}anje fluidnosti eritrocitne membrane nakon tretmana esencijalnim uljem biljke Vitex agnus-castus, {to mo`e biti ko ristan efekat pri tretm...

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Electron Paramagnetic Resonance - A Powerful Tool of Medical Biochemistry in Discovering Mechanisms of Disease and Treatment Prospects

Cited by 15 publications

References 124 publications

Complexity of free radical Metabolism in human Erythrocytes

Complexity of free radical Metabolism in human Erythrocytes

Changes in oxidative stress intensity in blood of tumor-bearing rats following different modes of administration of rhenium-platinum system

Vitex Agnus-Castus L. Essential Oil Increases Human Erythrocyte Membrane Fluidity

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