Electron Paramagnetic Resonance Spectroscopy in the Liquid Phase

Gescheidt, Georg

doi:10.1002/9780470432235.ch4

Cited by 3 publications

(1 citation statement)

References 57 publications

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“…Each spectrum represents an average of results from ten scans. By analyzing the spectrum of 5-DSA, an order parameter can be calculated from the outer and inner hyperfine splitting 2T // and 2T t respectively, from which the rotational correlation time of the spin probe and fluid-torigid membrane ratio are derived (Gescheidt 2009). …”

Section: Plasma Membrane Fluidity Analysis By Electron Paramagnetic Rmentioning

confidence: 99%

Distinct pathways of cholesterol biosynthesis impact on insulin secretion

Zuniga-Hertz

Rebelato

Kassan

et al. 2014

Journal of Endocrinology

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Results from previous investigations have indicated that glucose-stimulated insulin secretion (GSIS) is affected by changes in cholesterol and its intermediates, but the precise link between secretion and cholesterol has not been thoroughly investigated. In this study, we show the contribution of both protein isoprenylation and cholesterol-dependent plasma membrane structural integrity to insulin secretion in INS-1E cells and mouse islets. Acute (2 h) inhibition of hydroxyl-methylglutaryl-CoA reductase by simvastatin (SIM) resulted in inhibition of GSIS without reduction in total cellular cholesterol content. This effect was prevented by cell loading with the isoprenyl molecule geranylgeranyl pyrophosphate. Chronic (24 h) inhibition of cholesterol biosynthesis resulted in inhibition of GSIS with a significant reduction in total cellular cholesterol content, which was also observed after the inhibition of cholesterol biosynthesis downstream of isoprenoid formation. Electron paramagnetic resonance analyses of INS-1E cells showed that the SIM-induced reduction in cholesterol increased plasma membrane fluidity. Thus, the blockade of cholesterol biosynthesis resulted in the reduction of availability of isoprenoids, followed by a reduction in the total cholesterol content associated with an increase in plasma membrane fluidity. Herein, we show the different contributions of cholesterol biosynthesis to GSIS, and propose that isoprenoid molecules and cholesterol-dependent signaling are dual regulators of proper b-cell function.

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Section: Plasma Membrane Fluidity Analysis By Electron Paramagnetic Rmentioning

confidence: 99%

Distinct pathways of cholesterol biosynthesis impact on insulin secretion

Zuniga-Hertz

Rebelato

Kassan

et al. 2014

Journal of Endocrinology

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Pulsed Methods in Electron Spin Resonance

Bowman

2010

Encyclopedia of Analytical Chemistry

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This article describes how pulsed electron spin resonance (ESR) signals and spectra are generated. A few basic pulse sequences are introduced, along with a simple vector model and the Bloch equations to explain the signals that are produced and the type of information each signal contains. The major applications of pulsed ESR are described for the measurement of ESR spectra, relaxation times, chemical reaction kinetics, molecular motion, hyperfine interactions, and the distances between radicals. The common pulse sequences used for each of these measurements are described and some of the limitations and drawbacks are discussed. The simple models for pulsed ESR are modified, as needed, to explain unexpected features encountered in some experimental measurements. Double resonance and 2D spectroscopies such as electron nuclear double resonance (ENDOR), double electron–electron resonance (DEER), and hyperfine sublevel correlation (HYSCORE) are discussed.

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ChemInform Abstract: Electron Paramagnetic Resonance Spectroscopy in the Liquid Phase

Gescheidt

2010

ChemInform

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Electron Paramagnetic Resonance Spectroscopy in the Liquid Phase

Cited by 3 publications

References 57 publications

Distinct pathways of cholesterol biosynthesis impact on insulin secretion

Distinct pathways of cholesterol biosynthesis impact on insulin secretion

Pulsed Methods in Electron Spin Resonance

ChemInform Abstract: Electron Paramagnetic Resonance Spectroscopy in the Liquid Phase

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