Electron Spin Resonance (ESR) for the study of Reactive Oxygen Species (ROS) on the isolated frog skin (Pelophylax bergeri): A non-invasive method for environmental monitoring

D’Errico, Gerardino; Vitiello, Giuseppe; Tommaso, Gaetano De; Abdel‐Gawad, Fagr Kh.; Brundo, Maria Violetta; Ferrante, Margherita; Maio, Anna De; Trocchia, S.; Bianchi, Anna Rita; Ciarcia, Gaetano; Guerriero, Giulia

doi:10.1016/j.envres.2018.03.044

Cited by 47 publications

(29 citation statements)

References 48 publications

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“…By supplementing with antioxidants, CAT activity inhibition was controlled. Our results seem to be supported by similar studies (Afifi, Saddick, & Abu Zinada, ; D’Errico et al, ; Figueroa et al, ).…”

Section: Discussionsupporting

confidence: 91%

The protective effects of Vitamin C and Trolox on kinematic and oxidative stress indices in rainbow trout sperm cells against flower‐like ZnO nanoparticles

Özgür

2019

Aquac Res

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This study investigated the protective effects of 1 mM Vitamin C and 1 mM Trolox on kinematic and oxidative stresses in rainbow trout (Oncorhynchus mykiss) sperm cells against flower‐like zinc oxide nanoparticles (ZnO‐NP, 1 mg/L) in vitro, during 2 hr. Trolox showed protective effects on all kinematic parameters measured for sperm cells against ZnO‐NPs, whereas Vitamin C only affected angular path velocity (VAP), linearity (LIN) and amplitude of lateral displacement of the sperm cell head (ALH). Although ZnO‐NPs reduced total glutathione (TGSH) and catalase (CAT) compared with the control, there were positive treatment effects for both Trolox and Vitamin C on antioxidant capacity. Conversely, while ZnO‐NP increased the rate of malondialdehyde (MDA) lipid peroxidation, it decreased following antioxidant therapy. This research suggests that Trolox and Vitamin C play a protective role for rainbow trout sperm exposed to ZnO‐NP.

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Section: Discussionsupporting

confidence: 91%

The protective effects of Vitamin C and Trolox on kinematic and oxidative stress indices in rainbow trout sperm cells against flower‐like ZnO nanoparticles

Özgür

2019

Aquac Res

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“…Studies on Amphibia Anura [78,79,81,82,125] and in particular on Ranidae, the Italian pool frog Pelophylax bergeri, showed how temperature fluctuations and pollutants can affect the antioxidant defense [123,126]. In fact, a large study (2011-2016) carried out along the coasts of Sarno (Italy) highlighted how a contaminated site produces radical content in Pelophylax bergeri skin tissues.…”

Section: Amphibiamentioning

confidence: 99%

Antioxidative Defense and Fertility Rate in the Assessment of Reprotoxicity Risk Posed by Global Warming

Parisi

Guerriero

2019

Antioxidants

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The objective of this review is to briefly summarize the recent progress in studies done on the assessment of reprotoxicity risk posed by global warming for the foundation of strategic tool in ecosystem-based adaptation. The selected animal data analysis that was used in this paper focuses on antioxidative markers and fertility rate estimated over the period 2000–2019. We followed a phylogenetic methodology in order to report data on a panel of selected organisms that show dangerous effects. The oxidative damage studies related to temperature fluctuation occurring in biosentinels of different invertebrate and vertebrate classes show a consistently maintained physiological defense. Furthermore, the results from homeothermic and poikilothermic species in our study highlight the influence of temperature rise on reprotoxicity.

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“…Samples were fixed in 4% formalin. After washing in PBS, the samples were dehydrated in ascending series of ethanol and embedded in paraffin (Brundo et al, 2011;D'Errico et al, 2018;Guerriero et al, 2018;Marchini, Brundo, Sottile, & Viscuso, 2009). Morphological staining was carried out on histological sections (4 μm thick) using HE/Hematoxylin/Eosin (Bio-Optica).…”

Section: Histologymentioning

confidence: 99%

Toxicity assessment of nano‐TiO₂inApis melliferaL., 1758: histological and immunohistochemical assays

Ferrara

Salvaggio

Pecoraro

et al. 2019

Microscopy Res & Technique

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The aim of this study was to evaluate the toxicity of titanium dioxide nanoparticles (TiO2NPs) by short‐term toxicity tests in Apis mellifera, considered an excellent bioindicator organism mainly due to its sensitivity. Bees have been exposed to several concentrations of TiO2NPs (1 × 10−3, 1 × 10−4, 1 × 10−5, 1 × 10−6 mg/10 ml) for 10 days. Morphostructural and histological assays were done on gut and honey sac. The research of exposure biomarkers like metallothioneins 1 (MT1) and Heat Shock Protein 70 (HSP70) was performed to verify if a detoxification mechanism has been activated in the exposed animals. No histological alteration on the epithelium of the gut and honey sac were observed in exposed samples. A significant positivity for anti‐MT1 antibody was observed only in the honey sac cells. A weak positivity for HSP70 was observed in both structures analyzed. In several studies have shown the non‐toxicity of TiO2NPs on other model organisms, in our study, titanium dioxide nanoparticles was proven to be highly toxic at the highest concentration tested (100% of lethality to 1 × 10−3 mg/10 ml) and moderately toxic at lower concentrations. Honey bees proved to be excellent models for study of NPs toxicity and for monitoring environment.

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Electron Spin Resonance (ESR) for the study of Reactive Oxygen Species (ROS) on the isolated frog skin (Pelophylax bergeri): A non-invasive method for environmental monitoring

Cited by 47 publications

References 48 publications

The protective effects of Vitamin C and Trolox on kinematic and oxidative stress indices in rainbow trout sperm cells against flower‐like ZnO nanoparticles

The protective effects of Vitamin C and Trolox on kinematic and oxidative stress indices in rainbow trout sperm cells against flower‐like ZnO nanoparticles

Antioxidative Defense and Fertility Rate in the Assessment of Reprotoxicity Risk Posed by Global Warming

Toxicity assessment of nano‐TiO₂inApis melliferaL., 1758: histological and immunohistochemical assays

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Electron Spin Resonance (ESR) for the study of Reactive Oxygen Species (ROS) on the isolated frog skin (Pelophylax bergeri): A non-invasive method for environmental monitoring

Cited by 47 publications

References 48 publications

The protective effects of Vitamin C and Trolox on kinematic and oxidative stress indices in rainbow trout sperm cells against flower‐like ZnO nanoparticles

The protective effects of Vitamin C and Trolox on kinematic and oxidative stress indices in rainbow trout sperm cells against flower‐like ZnO nanoparticles

Antioxidative Defense and Fertility Rate in the Assessment of Reprotoxicity Risk Posed by Global Warming

Toxicity assessment of nano‐TiO2inApis melliferaL., 1758: histological and immunohistochemical assays

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Toxicity assessment of nano‐TiO₂inApis melliferaL., 1758: histological and immunohistochemical assays