Electron spin resonance spectroscopy

Freeman, Harold S.; Bereman, Robert D.

doi:10.1007/978-94-011-1358-8_5

Analytical Chemistry of Synthetic Colorants 1995

DOI: 10.1007/978-94-011-1358-8_5

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Electron spin resonance spectroscopy

Harold S. Freeman¹,

Robert D. Bereman²

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2003

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Singlet Oxygen Detection in Skim Milk by Electron Spin Resonance Spectroscopy

Bradley

Lee

Min

2003

Journal of Food Science

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2,2,6,6-Tetramethyl-4-piperidone (TMPD) can react with singlet oxygen to produce the corresponding nitroxide, 2,2,6,6-tetramethyl-4-piperidone-1-oxyl (TAN), which can be detected by electron spin resonance (ESR) spectroscopy. ESR detected the formation of TAN in 5 mM riboflavin phosphate buffer, or skim milk containing 20 mM TMPD during illumination. The effects of illumination, riboflavin, and oxygen on the formation of TAN indicated that the presence of each was required for singlet oxygen formation. The photoreduced riboflavin might reduce TAN to the hydroxylamine of TAN, thus decreasing the paramagnetic TAN signal. Maximum concentration of TAN was obtained at an added 10 mM riboflavin in buffer solution or in skim milk after 15 min of illumination in the presence of oxygen.

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Singlet Oxygen Detection in Skim Milk by Electron Spin Resonance Spectroscopy

Bradley

Lee

Min

2003

Journal of Food Science

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Electron spin resonance spectroscopy

Cited by 1 publication

References 15 publications

Singlet Oxygen Detection in Skim Milk by Electron Spin Resonance Spectroscopy

Singlet Oxygen Detection in Skim Milk by Electron Spin Resonance Spectroscopy

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