Electron spin resonance study of changes in implanted muscle: a model for implanted tumours

Dodd, Nicholas J F; Silcock, Judith

doi:10.1088/0143-0815/1/3/306

Cited by 2 publications

(3 citation statements)

References 9 publications

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“…We believe that while a very low concentration of ascorbyl radicals may be produced during normal metabolism, the appearance of the doublet signal largely represents release of ascorbic acid from damaged tissue. This is consistent with the observed correlation between the doublet signal and the NO-haemoprotein signals in tissue (Dodd & Silcock, 1980). While in effect an artifact, the ascorbyl radical signal in fresh samples of an implanted tumour can be related indirectly to the rate of tumour growth.…”

Section: Discussionsupporting

confidence: 89%

“…The narrow doublet signal, seen only in unfrozen samples of tissue has previously been assigned to the ascorbyl radical (Dodd, 1973). This signal, although not detected in normal muscle, is readily observed in implanted tumours (Dodd & Silcock, 1976) and implants of normal tissue (Dodd & Silcock, 1980). It has now been shown that the signal is produced in these tissues by aerobic oxidation of ascorbic acid.…”

Section: Discussionmentioning

confidence: 94%

“…A recent preliminary report (Dodd & Swartz, 1980) on the nature of the "lyophilization signal" provided a possible explanation for ©) The Macmillan Press Ltd., 1984 these differences. It was shown, using implanted muscle as a model for implanted tumours (Dodd & Silcock, 1980), that the magnitude of the "lyophilization signal" in tissue can be related to the availability of ascorbic acid, as a result of cellular damage. This study has been extended and is reported here in more detail.…”

mentioning

confidence: 99%

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The nature of the ESR signal in lyophilized tissue and its relevance to malignancy

Dodd¹,

Swartz²

1984

Br J Cancer

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Comparison of 9 and 35 GHz spectra, obtained from frozen and lyophilized tissues, with those from model systems containing ascorbic acid, confirm that the major component of the "lyophilization signal" of tissue is the ascorbyl radical, stabilized by adsorption on an inert matrix. The magnitude of the signal under anoxic conditions is shown to be a measure of cellular damage, which allows intracellular ascorbic acid to be oxidized. On exposure of lyophilized samples to air, the signal increases due to autoxidation of the available tissue ascorbic acid. Under moist atmospheric conditions the ascorbyl radicals readily decay, leaving other radicals, which appear to be formed by interaction of ascorbic acid or ascorbyl radicals with some tissue component. The results show that, although widely studied, the free radical ESR signal of lyophilized tissue is not unique to tumour and has no relevance to malignancy.