Electron spin resonance study of changes during development of solid Yoshida tumour. I: Ascorbyl radical

Silcock, Judith; Dodd, Nicholas J F

doi:10.1038/bjc.1976.210

Cited by 11 publications

(11 citation statements)

References 6 publications

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“…The secondary changes occurring in the blood are only detected in the developing leukaemias and are probably associated with cellular breakdown and release of iron, which is then taken up by transferrin. The changes observed in the blood of the leukaemic mice are, however, quite different from those in the blood of rats after implantation of a solid Yoshida tumour (Dodd and Silcock, 1976), but are similar to the changes in the blood of rats with developing Yoshida ascites tumours (unpublished). In the case of the solid tumour, no change is detectable in the blood within the first 4 days of implantation owing, we believe, to an initial lack of adequate blood supply to the tumour.…”

Section: Discussionsupporting

confidence: 55%

“…About 5 animals were examined for each experimental point. The spectrometer was operated, in conjunction with a Nicolet 1020A signal averager, at conditions previously described (Dodd & Giron-Conland, 1975;Silcock & Dodd, 1976). The spectra were quantitated by recording the relative heights of the ascorbyl-radical signal and the manganese standard signal.…”

Section: Methodsmentioning

confidence: 99%

“…More recently, experiments have examined the changes in the concentration of paramagnetic metal ions (Swartz et al, 1973;Dodd, 1975;Dodd & Silcock, 1976) and a particular free radical, the ascorbyl radical (Dodd, 1973;Dodd & GironConland, 1975;Giron-Conland, 1975;Silcock & Dodd, 1976) during the development of experimental malignancies in mice and rats. This paper reports ESR studies on a murine myeloid leukaemia carried in RFM/Un Inice, and compares the results with those obtained in RF/J mice.…”

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confidence: 99%

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ESR study of development of RFM/Un murine myeloid leukaemia

Dodd¹,

Silcock²

1978

Br J Cancer

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The blood, spleen and liver of RFM/Un mice were examined by means of electron spin resonance (ESR) throughout the course of myeloid leukaemia induced by i.v. injection of leukaemic spleen cells. Marked changes in the concentration of iron transferrin and caeruloplasmin were observed in the blood 1 day after injection. As the disease progressed, changes occurred in the concentrations of the ascorbyl radical and of paramagnetic metal complexes in both spleen and liver. These changes are compared with those induced in RF/J mice injected with normal and leukaemic spleen cells from RFM/Un mice.

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Section: Discussionsupporting

confidence: 55%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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ESR study of development of RFM/Un murine myeloid leukaemia

Dodd¹,

Silcock²

1978

Br J Cancer

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“…The ESR signal intensity of the AscR is taken as an indicator of oxidative stress due to oxidation of intracellular ascorbic acid leading to cellular damage (Dodd and Swartz 1984). AscR signal, although not detected in normal muscle, is readily observed in implanted tumors in vivo (Silcock and Dodd 1976). Our results suggest that hyperthermia could accelerate oxidation of Asc in HSC-4 cell suspension, which resulted in anti-proliferative effect and cellular damage.…”

Section: Discussionmentioning

confidence: 73%

Anticancer effects of 6-o-palmitoyl-ascorbate combined with a capacitive-resistive electric transfer hyperthermic apparatus as compared with ascorbate in relation to ascorbyl radical generation

et al. 2011

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The aim of the present study is to determine the anti-proliferative activity of 6-o-palmitoyl-L-ascorbic acid (Asc6Palm) that is a lipophilic derivative of L-ascorbic acid (Asc), on human tongue squamous carcinoma HSC-4 cells by combined use of hyperthermia in comparison to Asc. Asc6Palm or Asc were administered to HSC-4 cells for 1 h, to which hyperthermia at 42°C was applied for initial 15 min. After further 1-72 h incubation at 37°C, cell proliferation was determined with Crystal Violet staining. Ascorbyl radical (AscR) in HSC-4 cell suspension was measured by electron spin resonance (ESR), and cell morphology was observed with scanning electron microscopy (SEM). At 37°C, 4 mM Asc or 0.35 mM Asc6Palm were enough to suppress proliferation of HSC-4 cells. By combined use of hyperthermia at 42°C, cell proliferation was decreased when compared to 37°C. After Asc of 4 mM was incubated with HSC-4 cell suspensions at 37°C or 42°C for 0-180 min, the signal intensity of ascorbyl radical (AscR) by ESR was not different regardless of the presence or absence of cells at 37°C, whereas AscR signal was enlarged in the presence of HSC-4 cells at 42°C. It was suggested that oxidation of Asc occurred rapidly in HSC-4 cells by hyperthermia, and thereby enhanced the anti-proliferative activity. By SEM observation, the surface of HSC-4 cells treated with Asc6Palm revealed distinct morphological changes. Thus, the combined regimen of Asc6Palm and hyperthermia is expected to exert a marked antitumor activity.

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“…This paper reports the sequential changes occurring in the concentrations of the paramagnetic metal ions in frozen tissue samples taken from rats carrying a Yoshida tumour. The work was undertaken in conjunction with a study of the ascorbyl radical in fresh tissue (Silcock and Dodd, 1976).…”

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confidence: 99%

Electron spin resonance study of changes during development of solid yoshida tumour. II. Paramagnetic metal ions

Dodd¹,

Silcock²

1976

Br J Cancer

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Summary.-Electron spin resonance (ESR) spectroscopy was used to examine changes in the concentrations of paramagnetic metal ions in Yoshida tumours carried by female Wistar rats. Blood, spleen and lymph nodes from these animals were also examined by ESR. A decrease in the concentration of a paramagnetic species associated with mitochondrial activity, and marked increases in those thought to be associated with inflammatory or immune reactions and cell lysis, were observed in the tumours within one day of implantation. During development of the tumour, and during its regression after treatment with methylene dimethane sulphonate (MDMS), further changes were observed in the concentrations of the species. These were dependent on the region of the tumour examined. In blood, development of the tumour produced an increase in ceruloplasmin and a decrease in iron-transferrin. An increase in spleen weight, as the tumour developed, was accompanied by a small decrease in the concentration of species with g-values of 6-0 and 4-3, which was reversed on regression of the treated tumour. The presence of metastases in the regional lymph nodes produced readily distinguishable changes in their ESR spectra.

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Electron spin resonance study of changes during development of solid Yoshida tumour. I: Ascorbyl radical

Cited by 11 publications

References 6 publications

ESR study of development of RFM/Un murine myeloid leukaemia

ESR study of development of RFM/Un murine myeloid leukaemia

Anticancer effects of 6-o-palmitoyl-ascorbate combined with a capacitive-resistive electric transfer hyperthermic apparatus as compared with ascorbate in relation to ascorbyl radical generation

Electron spin resonance study of changes during development of solid yoshida tumour. II. Paramagnetic metal ions

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