Electron transfer-based antioxidant capacity assays and the cupric ion reducing antioxidant capacity (CUPRAC) assay

Apak, Reşat

doi:10.1002/9781119135388.ch4

Cited by 17 publications

(20 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mechanism of betanin oxidation. ( a ) More O’Ferrall–Jencks diagrams showing concurrent PCET processes of a generic compound XH [64,65], and the parameters that can be used for mechanistic interpretation, i.e. p K a s, reduction potentials ( E o ), proton affinity (PA), proton dissociation energy (PDE), ionization potential (IP), electron transfer enthalpy (ETE), and homolytic bond dissociation enthalpy (BDE).…”

Section: Figurementioning

confidence: 99%

Rationale on the High Radical Scavenging Capacity of Betalains

Nakashima

Bastos

2019

Antioxidants

View full text Add to dashboard Cite

Betalains are water-soluble natural pigments of increasing importance as antioxidants for pharmaceutical use. Although non-phenolic betalains have lower capacity to scavenge radicals compared to their phenolic analogues, both classes perform well as antioxidants and anti-inflammatory agents in vivo. Here we show that meta-hydroxyphenyl betalain (m-OH-pBeet) and phenylbetalain (pBeet) show higher radical scavenging capacity compared to their N-methyl iminium analogues, in which proton-coupled electron transfer (PCET) from the imine nitrogen atom is precluded. The 1,7-diazaheptamethinium system was found to be essential for the high radical scavenging capacity of betalains and concerted PCET is the most thermodynamically favorable pathway for their one-electron oxidation. The results provide useful insights for the design of nature-derived redox mediators based on the betalain scaffold.

show abstract

Section: Figurementioning

confidence: 99%

Rationale on the High Radical Scavenging Capacity of Betalains

Nakashima

Bastos

2019

Antioxidants

View full text Add to dashboard Cite

show abstract

“…The Table 1 shows that DMC and DMD had more than twice the radical scavenging activity (% RSA) of DAC (31.97 ± 4.74%) with 72.66 ± 0.72 and 74.93 ± 0,65%, respectively. The reason why an increase in activity was not observed in this test when addressing DMD may be because the solvent is forming hydrogen bonds with the polyphenols, which would prevent its interaction with the DPPH radical due to steric hindrance (30). Moreover, the percentages obtained for DMC and DMD are higher than the reported for the antioxidant standard Trolox (53.4 ± 1.9%) and other antioxidants used in the food industry like butylated hydroxyanisole (22.3 ± 0,6%) and butylated hydroxytoluene (8.0 ± 0.4%) (31) while DAC had an RSA percentage lower than Trolox but higher than the synthetic antioxidants mentioned.…”

Section: Discussionmentioning

confidence: 95%

DNA barcode analysis and antioxidant activity of Desmodium molliculum and Desmodium adscendens

Sarmiento-Pacurucu,

Santos-Ordóñez,

León-Tamariz

et al. 2024

Preprint

View full text Add to dashboard Cite

In Ecuador, two species of Desmodium are used as traditional medicine. These are popularly known as “hierba del infante” and refer to D. adscendens and D. molliculum. The first species has a large base of information on its genetics and biological activity on which its traditional use is supported, while the second, D. molliculum, lacks this scientific information. This research aims to establish a base knowledge for the species D. molliculum: characterize the species genetically by obtaining the molecular DNA barcodes trnh-psbA, rbcL, matK, ITS1 and ITS2, evaluate its antioxidant effect and compare it with that of D. adscendens using the in vitro techniques of Folin-Ciocalteu, ABTS, DPPH and FRAP to assess its potential medicinal effect. De novo genetic DNA barcodes were obtained for the species D. molliculum and the phylogenetic analysis separated them from those obtained from D. adscendens, indicating that the analyzed species can be discriminated by DNA barcodes. In addition, the methanolic extracts of D. molliculum contain more than double the content of total polyphenols (30.1 ± 1.1 mg GAE/g dry plant) than those of D. adscendens (13.82 ± 0.74 mg AG/g dry plant) as well as better performance in all antioxidation assays. Additionally, this research established that the defatted extract of D. molliculum has the highest antioxidant activity in the ABTS (1.16 ± 0.001 mg TE/mg) and FRAP (0.39 ± 0.01 mg TE/mg) assays.

show abstract

“… Hydrogen peroxide scavenging and peroxynitrite scavenging activity assays establish the total oxidant scavenging capacity of antioxidants in fluorescence, using horseradish peroxidase (HRP) enzyme and scopoletin or dihydrorhodamine to measure the fluorescence intensity or inhibition [ 33 ]. The total peroxyl radical trapping antioxidant parameter (TRAP) calculates the antioxidant capacity to scavenge luminol-derived radicals, generated from AAPH decomposition in chemiluminescence [ 34 ]. A catalase assay (CAT) demonstrates the presence of catalase, an enzyme that dissociates hydrogen peroxide into molecular oxygen and water; the catalase activity is calculated based on the rate of decomposition of hydrogen peroxide, proportional to the reduction in the absorbance at 240 nm [ 35 ].…”

Section: Antioxidant Activity Future In Wound Healingmentioning

confidence: 99%

Exploring the Antioxidant Potential of Gellan and Guar Gums in Wound Healing

Dodi,

Sabau,

Crețu

et al. 2023

Pharmaceutics

View full text Add to dashboard Cite

It is acknowledged that the presence of antioxidants boosts the wound-healing process. Many biopolymers have been explored over the years for their antioxidant potential in wound healing, but limited research has been performed on gum structures and their derivatives. This review aims to evaluate whether the antioxidant properties of gellan and guar gums and wound healing co-exist. PubMed was the primary platform used to explore published reports on the antioxidant wound-healing interconnection, wound dressings based on gellan and guar gum, as well as the latest review papers on guar gum. The literature search disclosed that some wound-healing supports based on gellan gum hold considerable antioxidant properties, as evident from the results obtained using different antioxidant assays. It has emerged that the antioxidant properties of guar gum are overlooked in the wound-healing field, in most cases, even if this feature improves the healing outcome. This review paper is the first that examines guar gum vehicles throughout the wound-healing process. Further research is needed to design and evaluate customized wound dressings that can scavenge excess reactive oxygen species, especially in clinical practice.

show abstract

Electron transfer-based antioxidant capacity assays and the cupric ion reducing antioxidant capacity (CUPRAC) assay

Cited by 17 publications

References 80 publications

Rationale on the High Radical Scavenging Capacity of Betalains

Rationale on the High Radical Scavenging Capacity of Betalains

DNA barcode analysis and antioxidant activity of Desmodium molliculum and Desmodium adscendens

Exploring the Antioxidant Potential of Gellan and Guar Gums in Wound Healing

Contact Info

Product

Resources

About