Electron‐withdrawing inductive effects enhanced strategy for protein thiol sensing and blocking agent design

Abstract

It is a great challenge to discover novel chemical reactions suitable for biological analysis in a living system. The development of novel protein thiol blocking agents is a crucial need for exploring protein thiol functions in protein refolding, signal transduction, and redox regulation. We are always keen on seeking novel chemical reactions applied to endogenous biological macromolecules or protein thiol sensing, blocking, and labeling. In the present work, we have successfully developed a novel agent to block protein thiol by enhanced electron‐withdrawing inductive effects. This sensing and blocking process was detailedly monitored by UV‐vis, fluorescent spectra, and SDS‐Page gel separation. The spectral studies demonstrated that the agent could react ultrafastly with thiol within seconds at μM level. Furthermore, fluorescent imaging in cells and in vivo was further used for the validation of its ability to sensing and blocking thiol, providing evidence of downregulated protein thiols in Parkinson's disease. The enhanced electron‐withdrawing inductive effect strategy in this work may provide a general guideline for designing protein thiol agent.

Coordination Synergistic-Induced J-Aggregation Enhanced Fluorescent Performance of HBT-Excimers and Imaging Applications

“…In addition, we further investigated the probe HBT-Zn-red incubated with 6-OHDA under a shorter time (Figure S18), the fluorescent signal also showed a certain diminishment. Zinc ion loss may be ascribed to the reason that the sulfhydryl group of protein was destroyed by oxidative stress in the PD model, thereby leading to a loss of binding ability with zinc . All the results suggested that zinc ion was greatly reduced in the PD model by 6-OHDA stimuli, further indicating zinc ion plays an important role in maintaining redox balances.…”

“…Zinc ion loss may be ascribed to the reason that the sulfhydryl group of protein was destroyed by oxidative stress in the PD model, thereby leading to a loss of binding ability with zinc. 42 All the results suggested that zinc ion was greatly reduced in the PD model by 6-OHDA stimuli, further indicating zinc ion plays an important role in maintaining redox balances.…”