Electronic Cigarettes Enhance Replication of <i>Mycobacterium abscessus</i> in Airway Epithelial Cells

Korukonda, Anu; Zhang, Chongxu; Rodríguez, Luis Ernesto; Guerrero, Andrea; Campos, Michael; Holt, G.E.; Fregien, Nevis; Conner, Gregory E.; Mirsaeidi, Mehdi

doi:10.1165/rcmb.2018-0339le

Cited by 3 publications

(6 citation statements)

References 13 publications

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“…In vitro models of exposure corroborate these increases. Several studies have shown an inflammatory signal in epithelial cells after e-cigarette exposure, reporting increased release of IL-6 [42,[55][56][57][58], IL-8 [42,55,58,59] and MCP-1 [55]. Furthermore, HIGHAM et al demonstrated an increased IL-6 and IL-8 response in e-cigarette-exposed COPD epithelial cells, compared with healthy control cells.…”

Section: Cohort Studiesmentioning

confidence: 99%

“…Epithelial cells exposed to e-cigarette vapour in vitro are more vulnerable to intracellular and extracellular infection by mycobacterium [56] and S. aureus [43] respectively. WU et al shared similar findings in human rhinovirus (HRV) viral infection [57].…”

Section: Mucociliary Clearancementioning

confidence: 99%

“…An ordered and proportionate response to pathogenic stimulus is crucial for expulsion of pathogens without persistent inflammation. Several studies demonstrated increased inflammatory cytokine output by e-cigarette-exposed epithelial cells [56,95] and macrophages [104] in response to bacterial stimulus. Similarly, HRV infection caused IL-6 release from e-cigarette-exposed epithelial cells [57].…”

Section: Inflammatory Responsesmentioning

confidence: 99%

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Predicting the pulmonary effects of long-term e-cigarette use: are the clouds clearing?

et al. 2022

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Commercially available since 2007, e-cigarettes are a popular electronic delivery device of ever-growing complexity. Given their increasing use by ex-smokers, smokers and never-smokers, it is important to evaluate evidence of their potential pulmonary effects and predict effects of long-term use, since there has been insufficient time to study a chronic user cohort. It is crucial to evaluate indicators of harm seen in cigarette use, and those potentially unique to e-cigarette exposure. Evaluation must also account for the vast variation in e-cigarette devices (now including at least five generations of devices) and exposure methods used in vivo and in vitro.Thus far, short-term use cohort studies, combined with in vivo and in vitro models, have been used to probe for the effects of e-cigarette exposure. The effects and mechanisms identified, including dysregulated inflammation and decreased pathogen resistance, show concerning overlaps with the established effects of cigarette smoke exposure. Additionally, research has identified a signature of dysregulated lipid processing, which is unique to e-cigarette exposure.This review will evaluate the evidence of pulmonary effects of, and driving mechanisms behind, e-cigarette exposure, which have been highlighted in emerging literature, and highlight the gaps in current knowledge. Such a summary allows understanding of the ongoing debate into e-cigarette regulation, as well as prediction and potential mitigation of future problems surrounding e-cigarette use.

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Section: Cohort Studiesmentioning

confidence: 99%

Section: Mucociliary Clearancementioning

confidence: 99%

Section: Inflammatory Responsesmentioning

confidence: 99%

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Predicting the pulmonary effects of long-term e-cigarette use: are the clouds clearing?

et al. 2022

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“…Two days after adding the endothelial cells, the lung model was used for experiment and the media was changed every 2 days. This lung model has been previously published (14). For the current study, primary NHBE cells from five individuals were used to develop LOMM.…”

Section: Lung-on-membrane Model (Lomm)mentioning

confidence: 99%

Mycobacterium abscessus—Bronchial Epithelial Cells Cross-Talk Through Type I Interferon Signaling

et al. 2019

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“…In the setting of sarcoidosis, novel 3D models properly mimicking granuloma formation have not yet been created; this has contributed to the lack of available targeted therapies. We developed a novel lung on chip model by adding a microfluidics system to our previously developed dual-chambered lung on membrane model (LOMM) [26,27]. We also recently developed an in vitro sarcoidosis granulomas model [28].…”

Section: Introductionmentioning

confidence: 99%

Novel three-dimensional biochip pulmonary sarcoidosis model

et al. 2021

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Sarcoidosis is a multi-system disorder of granulomatous inflammation which most commonly affects the lungs. Its etiology and pathogenesis are not well defined in part due to the lack of reliable modeling. Here, we present the development of an in vitro three-dimensional lung-on-chip biochip designed to mimic granuloma formation. A lung on chip fluidic macrodevice was developed and added to our previously developed a lung-on-membrane model (LOMM). Granulomas were cultured from blood samples of patients with sarcoidosis and then inserted in the air-lung-interface of the microchip to create a three-dimensional biochip pulmonary sarcoidosis model (3D BSGM). Cytokines were measured after 48 hours. ELISA testing was performed to measure cytokine response difference between LOMM with 3D BSGM. There were statistically significant differences in IL-1ß (P = 0.001953), IL-6 (P = 0.001953), GM-CSF (P = 0.001953), and INF-γ expressions (P = 0.09375) between two groups. The current model represents the first 3D biochip sarcoidosis model created by adding a microfluidics system to a dual-chambered lung on membrane model and introducing developed sarcoid-granuloma to its air-lung-interface.

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Electronic Cigarettes Enhance Replication of Mycobacterium abscessus in Airway Epithelial Cells

Cited by 3 publications

References 13 publications

Predicting the pulmonary effects of long-term e-cigarette use: are the clouds clearing?

Predicting the pulmonary effects of long-term e-cigarette use: are the clouds clearing?

Mycobacterium abscessus—Bronchial Epithelial Cells Cross-Talk Through Type I Interferon Signaling

Novel three-dimensional biochip pulmonary sarcoidosis model

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