Objective: Almost a third of college students are obese, placing them at risk for adult obesity and its complications. Internet use may be one factor contributing to college student obesity. The purpose of this study was to examine associations of college student internet use with physical activity and fitness. Methods: Older adolescents between 18 and 23 years were recruited from a large university. Using experience sampling method, participants received 6 randomly-timed text message surveys for 7 days. Survey questions assessed whether they were currently online, for how long and current online activities. Participants also completed the International Physical Activity questionnaire and reported their body mass index. Multivariate models assessed the association of internet use with physical activity and fitness. Results: Among 189 participants, the mean age was 18.9 (SD = 0.9), 58.8% were female and most were Caucasian (90.5%). Greater internet use was associated with fewer days per week of vigorous intensity exercise (p < 0.001). Participants who spent less than 1 hour/day online reported a mean of 3.2 days per week of vigorous intensity exercise (SD = 2.0), those with 3 or more hours online daily reported 1.4 (SD = 2.1). Those who reported internet activities focused on academics reported increased days of vigorous intensity exercise compared to those who reported internet activity focused on social networking sites (p < 0.001). Conclusions: There were no significant associations between internet use time and BMI. Findings suggest that both online time and particular online activities may be associated with decreased vigorous physical activity. Future efforts should consider reframing internet use guidelines for this population around both time and activities.