Electronic tongue for simultaneous detection of endotoxins and other contaminants of microbiological origin

Heras, Jorge Yánez; Pallarola, Diego; Battaglini, Fernando

doi:10.1016/j.bios.2010.04.004

Cited by 58 publications

(18 citation statements)

References 34 publications

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“…The electrochemical approach based on sensors or biosensors stands out among all of them. Most of these studies rely on electrochemical impedance spectroscopy (EIS) for the LPS detection (Cho et al, 2012;Ding et al, 2007;Heras et al, 2010;Rahman et al, 2013). For instance, the sensors developed by Ding and his co-workers and Rahman and its group use EIS to quantify the amount of LPS through immobilized PmB (polymyxin B).…”

Section: Introductionmentioning

confidence: 99%

Novel fully-integrated biosensor for endotoxin detection via polymyxin B immobilization onto gold electrodes

Zuzuarregui

Arana

Pérez-Lorenzo

et al. 2013

J. Sens. Sens. Syst.

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Abstract. In this paper an electrochemical endotoxin biosensor consisting of an immobilized lipopolysaccharide (LPS) ligand, polymyxin B (PmB), is presented. Several parameters involved both in the device fabrication and in the detection process were analyzed to optimize the ligand immobilization and the interaction between PmB and LPS, aiming at increasing the sensitivity of the sensor. Different electrochemical pre-treatment procedures as well as the functionalization methods were studied and evaluated. The use of a SAM (self-assembled monolayer) to immobilize PmB and the quantification of the interactions via cyclic voltammetry allowed the development of a robust and simple device for in situ detection of LPS. Thus, the biosensor proposed in this work intends an approach to the demanding needs of the market for an integrated, portable and simple instrument for endotoxin detection.

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Section: Introductionmentioning

confidence: 99%

Novel fully-integrated biosensor for endotoxin detection via polymyxin B immobilization onto gold electrodes

Zuzuarregui

Arana

Pérez-Lorenzo

et al. 2013

J. Sens. Sens. Syst.

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“…Affinity interactions had first been shown to result in conductivity increase. This effect allows discriminating between specific and unspecific processes in contrast to majority of impedimetric affinity sensors based on conductivity decrease as an analytical response .…”

Section: Figurementioning

confidence: 99%

Reagentless Microsensor Based on Conducting Poly(3‐aminophenylboronic Acid) for Rapid Detection of Microorganisms in Aerosol

et al. 2017

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We report on microsensor for direct detection of microorganisms in aerosol. Microsensor is based on interdigitated ultramicroelectrodes modified with electropolymerized 3‐aminophenylboronic acid (3‐APBA). Appearance of Penicillium chrysogenum in aerosol as well as further increase of its concentration lead to poly(3‐APBA) conductivity increase found by impedance spectroscopy. According to Raman spectroscopy data, the presence of microorganism affects polymer conductivity similarly to binding of glucose to polymer which results in self‐doping phenomenon. The elaborated detection technique is 100 times faster comparing to agar plate cultivation: it requires less than 20 minutes for analysis. Dynamic range of microsensor includes the upper limit for non‐contaminated air in Russian hygienic standard (500 colony‐forming units per cubic meter). The first reported reagentless detection of Penicillium chrysogenum in aerosol by conductivity increase reveals a prospect for creation of a promising alternative to conventional techniques for detection of microorganisms.

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“…Endotoxins are responsible for the toxic effects that cause fevers, 1 septic shock, 2 and sepsis. 3,4 Biosensor based endotoxin detection has been widely investigated recently, 5,6 and many methods have been investigated to identify endotoxins: hydrophobic interactions, 7 localized surface plasmon resonance (LSPR), 8 mass spectrometry, 9 optical methods, 10 the electronic tongue, 11 the voltammetric method, 12 and electrochemistry. [13][14][15][16][17] However, these methods have some disadvantages, such as high costs, a long processing time and the requirement of labeled markers.…”

Section: Introductionmentioning

confidence: 99%

An aptamer-based shear horizontal surface acoustic wave biosensor with a CVD-grown single-layered graphene film for high-sensitivity detection of a label-free endotoxin

Pang

et al. 2020

Microsyst Nanoeng

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The thickness of the sensitive layer has an important influence on the sensitivity of a shear horizontal surface acoustic wave (SH-SAW) biosensor with a delay-line structure and lower layer numbers of graphene produce better sensitivity for biological detection. Therefore, a label-free and highly sensitive SH-SAW biosensor with chemical vapor deposition (CVD-)-grown single-layered graphene (SLG) for endotoxin detection was developed in this study. With this methodology, SH-SAW biosensors were fabricated on a 36°Y-90°X quartz substrate with a base frequency of 246.2 MHz, and an effective detection cell was fabricated using acrylic material. To increase the surface hydrophilicity, chitosan was applied to the surface of the SLG film. Additionally, the aptamer was immobilized on the surface of the SLG film by cross-linking with glutaraldehyde. Finally, the sensitivity was verified by endotoxin detection with a linear detection ranging from 0 to 100 ng/mL, and the detection limit (LOD) was as low as 3.53 ng/mL. In addition, the stability of this type of SH-SAW biosensor from the air phase to the liquid phase proved to be excellent and the specificity was tested and verified by detecting the endotoxin obtained from Escherichia coli (E. coli), the endotoxin obtained from Pseudomonas aeruginosa (P. aeruginosa), and aflatoxin. Therefore, this type of SH-SAW biosensor with a CVD-grown SLG film may offer a promising approach to endotoxin detection, and it may have great potential in clinical applications.

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Electronic tongue for simultaneous detection of endotoxins and other contaminants of microbiological origin

Cited by 58 publications

References 34 publications

Novel fully-integrated biosensor for endotoxin detection via polymyxin B immobilization onto gold electrodes

Novel fully-integrated biosensor for endotoxin detection via polymyxin B immobilization onto gold electrodes

Reagentless Microsensor Based on Conducting Poly(3‐aminophenylboronic Acid) for Rapid Detection of Microorganisms in Aerosol

An aptamer-based shear horizontal surface acoustic wave biosensor with a CVD-grown single-layered graphene film for high-sensitivity detection of a label-free endotoxin

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