Electrooxidation of NADH on Modified Screen-Printed Electrodes: Effects of Conducting Polymer and Nanomaterials

Şahin, Melike; Ayrancı́, Erol

doi:10.1016/j.electacta.2015.03.030

Cited by 46 publications

(19 citation statements)

References 38 publications

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“…In this research work the cofactor electrochemical behavior was studied using the PBS buffer (pH 7) with KCl (0.1 M) as the electrolyte. This pH was chosen because NADH is instable in highly alkaline and acidic solutions due to its rapid degradation [27].…”

Section: Resultsmentioning

confidence: 99%

“…In a screen-printing electrode prototype, the oxidation of NADH (0.4 mM) occurred in a potential range from +0.18 V to +0.44 V but the signals were not well-defined [34]. At a screen-printing electrodes modified with MWCNTs (multiwalled carbon nanotubes), or AuNPs (gold nanoparticles) or with PNRs (polyneutral red films), it was possible to verify that the best response of the redox system NAD + /NADH was obtained with the modification using MWCNTs, which was used as an amperometric NADH detector [27]. The goal of this report is to present a sensor that can be easily fabricated, which is cheaper and environmentally friend and allows the electrochemical study of the redox couple NADH/NAD + .…”

Section: David Publishingmentioning

confidence: 99%

“…Another strategy is modification of the electrode surface with a polymeric substance, using electrodes modified with carbon nanotubes, nanofibers or using enzymatic methods that follow bioelectrocatalytic reaction [2,4,13,15,16,[20][21][22][23]. Investigation in this area usually explores the mediator use or the surface modification to improve the electrochemical detection [15,21,[24][25][26][27][28]. Some literature has been published related to the study of the redox couple NADH/NAD + in screen printing electrodes [1,4,21,[24][25][26][27][29][30][31][32][33][34].…”

Section: David Publishingmentioning

confidence: 99%

“…Investigation in this area usually explores the mediator use or the surface modification to improve the electrochemical detection [15,21,[24][25][26][27][28]. Some literature has been published related to the study of the redox couple NADH/NAD + in screen printing electrodes [1,4,21,[24][25][26][27][29][30][31][32][33][34]. The sensor built using this technique can incorporate the three electrodes (working electrode, reference and counter electrode), can be easily produced and miniaturized, work with a minimum volume (2 µL), it needs low reagent consumption, it can be fabricated in many supports (paper, glass) and sensors are disposable devices that can be used in many science fields [26,35].…”

Section: David Publishingmentioning

confidence: 99%

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A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System

Ribau¹,

Fortunato²

2018

JPP

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Abstract:A simple device which incorporates three electrodes (working electrode, counter electrode and reference electrode) was constructed to be used currently in laboratories without elevated cost. It does not need more than 2 µL of electrolyte, sample or working solution, his support material is paper, and the working electrode which is based on carbon ink can incorporate enzymes and cofactors. To test this concept we started this investigation using the NADH/NAD + redox couple which is an omnipresent coenzyme in living systems but is also a challenge to electrochemistry. The paper sensor fabrication was simple, rapid and cheaper. NADH was incorporated in the carbon ink by mixing both and, this mixture was used to print the working electrode. The direct electrochemical system NADH/NAD + signal obtained, using this device, appeared at low potentials. A quasi-reversible diffusional redox process was achieved and regeneration of the NADH after oxidation was reached. This small paper device was not only used to study the redox process of NAD + /NADH, but also its behavior in the presence of electroactive (ascorbic acid) and non-eletroactive species (glucose).

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Section: Resultsmentioning

confidence: 99%

Section: David Publishingmentioning

confidence: 99%

Section: David Publishingmentioning

confidence: 99%

Section: David Publishingmentioning

confidence: 99%

See 2 more Smart Citations

A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System

Ribau¹,

Fortunato²

2018

JPP

View full text Add to dashboard Cite

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“…However, it is also a challenge to develop biosensors, once there is the possibility of NAD + freely lives the enzyme, becoming the enzyme in an inactive conformation, but also the formation of inactive products (NAD 2 dimer). Another problem is that the oxidation of NADH is usually irreversible, it occurs at high potential, NAD + act as an inhibitor of the direct electrode process or even provokes electrode surface poisoning [21][22][23][24]. To overcome these difficulties, efforts have been made using different strategies as surface modification, use of mediators [13,16,[25][26][27] or use new materials (carbon nanofibers, carbon nanotubes, graphene and composites) as immobilization matrix for biomolecules [18,19,22,[28][29][30][31][32][33][34].…”

mentioning

confidence: 99%

Use of a Simple Fabrication Process to Produce a Biosensor: The 3-Hydroxybutyrate Dehydrogenase Case

Ribau¹,

Fortunato²

2018

JPP

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This study was aimed to construct a biodegradable but reliable 3-β-hydroxybutyrate biosensor. In this context a versatile paper based biosensor, quickly, easily and cheaply fabricated is reported. The procedure of fabrication is based on the assumption that the introduction of the enzyme in the carbon ink will allow enzyme stabilization and facilitate the study of the catalysis of enzymes and the detection of substrates. To prove this concept we use the enzyme 3-hydroxybutyrate dehydrogenase, in aqueous solution. This enzyme was chosen because it catalyzes the 3-β-hydroxybutyrate, which results from ketoacidosis. The quantification this substance in the diabetics' blood is very important as it can increase the reliability of the diagnosis of glycaemia. To prove the multi-use of this biosensor we not only study the redox process in steady state and during the catalytic process, but also detected and quantify the 3-β-hydroxybutyrate. Our results showed that it was possible to study the redox process that occurred during the catalysis and to confirm the amino acid residues that participate in it. It was also observed that glucose and ascorbic acid can interfere in the detection and quantification of the 3-β-hydroxybutyrate, what should be in mind when the quantification of the 3-β-hydroxybutyrate is made in blood samples.

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Electrochemical Behaviour of Microwave‐assisted Oxidized MWCNTs Based Disposable Electrodes: Proposal of a NADH Electrochemical Sensor

et al. 2018

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The effect of the oxidation degree of multiwalled carbon nanotubes (MWCNTs) for the detection of NADH was evaluated in this paper. MWCNTs were oxidized by microwave‐assisted sulfonitic treatment at different times (5, 10, 15, and 30 min) and deposited onto a graphite screen printed electrodes. Oxidized MWCNTs were characterized and the electrochemical performance evaluated. The best sensor in terms of sensitivity and stability was obtained after 15 minutes of oxidation (SPE/CNT15). A significant reduction of the NADH oxidation potential was recorded for oxidized MWCNTs compared with unmodified MWCNTs (0.270 V and 0.500 V, respectively vs. Ag/AgCl pseudo reference electrode), increasing the selectivity of the system. Chronoamperometric calibration curves carried out applying a potential of 0.3 V for 1 min were linear in the 4–35 μM range of NADH. A limit of detection of 1 μM was achieved with negligible surface fouling (three consecutive calibration curves, 30 total measurements: slope decrease 5.9 %). Inter electrode reproducibility (n=13) was good resulting in RSD of 15.2 % and 5.0 % for the peak intensity and the oxidation potential, respectively. Quantification of glucose in white wine samples was carried out to demonstrate the ability of the NADH sensor to work in real samples. A good correlation with a spectrophotometric kit for the glucose quantification was achieved.

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Electrooxidation of NADH on Modified Screen-Printed Electrodes: Effects of Conducting Polymer and Nanomaterials

Cited by 46 publications

References 38 publications

A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System

A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System

Use of a Simple Fabrication Process to Produce a Biosensor: The 3-Hydroxybutyrate Dehydrogenase Case

Electrochemical Behaviour of Microwave‐assisted Oxidized MWCNTs Based Disposable Electrodes: Proposal of a NADH Electrochemical Sensor

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