"Electropherogram"

Searcy, R.L.; Bergquist, L.M.

doi:10.1016/s0140-6736(60)90918-1

Cited by 3 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Total cholesterol was determined according to Searcy and Bergquist [23] in the whole extracts and in chloroform eluates from the column. Estimation of total neutral lipids was carried out by the charring procedure of Marsh and Weinstein [24] in chloroform eluates from the column.…”

Section: Lip Id Analysismentioning

confidence: 99%

Changes in the Structure, Composition and Function of Sarcoplasmic-Reticulum Membrane during Development

et al. 1975

View full text Add to dashboard Cite

The structure, chemical composition and function of the microsomal fraction, isolated by differential centrifugation and purified on sucrose gradients, from muscle of fetal, newborn and young rabbits were characterized and compared with those of sarcoplasmic reticulum vesicles from adult muscle. Negative staining shows that the microsomal vesicles isolated from muscles of embryos and newborn animals are smooth, in contrast to vesicles obtained from adult muscle which contain 4‐nm particles on their surface. The particles appear first in the microsomal vesicles from muscles of 5–8‐day‐old rabbits. Their number increases with the age of the animals. Ca2+‐pump protein, with molecular weight about 100000, accounts for 10% of the total protein content in sarcoplasmic reticulum membrane, isolated at the earliest stages of development analysed. Its amount increases continuously with the rabbit's age to the adult value of about 70% of total sarcoplasmic reticulum protein. The low amount of 100000‐dalton protein and lack of 4‐nm surface particles in sarcoplasmic reticulum vesicles obtained from fetal and newborn rabbits are strictly correlated with the low activity of Ca2+‐dependent ATPase and the ability to take up Ca2+. These activities rise in parallel with the age of the rabbits. On the other hand, Mg2+‐dependent ATPase activity is very high at the early stages of development and declines continuously to a low value in sarcoplasmic reticulum from adult muscle. The sarcoplasmic reticulum membrane from fetal and newborn rabbits contains a higher amount of lipids as compared with the membrane present in the muscle of adult animals. The ratio of both phospholipid to protein and neutral lipid to protein decreases with the age of the rabbits. The composition of sarcoplasmic reticulum phospholipid also changes during development.

show abstract

Section: Lip Id Analysismentioning

confidence: 99%

Changes in the Structure, Composition and Function of Sarcoplasmic-Reticulum Membrane during Development

et al. 1975

View full text Add to dashboard Cite

show abstract

“…Group IV was sacrificed after 6 months on the cholesterol-containing diet, and these birds were used to establish the extent to which cholesterol aggravates development of gross atherosclerosis (previous studies have shown (4)). At termination of the experiment the birds were sacrificed by decapitation and blood was collected for determination of serum cholesterol by the method of Searcy et a L ( 5 ) . Group VI was treated exactly as was Group IV except that at the end of 6 months the birds were taken off the cholesterol-containing ration and maintained for an additional 1 2 5 months on a diet of pigeon pellets alone.…”

mentioning

confidence: 99%

“…The level of aorta cholesterol was determined on aliquots of the extract by the Searcy procedure (5). The level of aorta cholesterol was determined on aliquots of the extract by the Searcy procedure (5).…”

mentioning

confidence: 99%