Electrophoresis and sudan black staining of lipoproteins on gelatinised cellulose acetate

Colfs, B.; Verheyden, Jan

doi:10.1016/0009-8981(67)90027-7

Cited by 50 publications

(8 citation statements)

References 3 publications

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“…LDL was prepared by discontinuous density gradient ultracentrifugation as described previously (19). The LDL was washed at a density of 1.063 g/ml and dialyzed against 150 mM NaCl, 1 Lipoproteins were analyzed for possible changes in their charge by lipoprotein electrophoresis on agarose (27). Vitamin E content of the lipoproteins was analyzed with the bathophenantroline-ferric chloride assay (28).…”

Section: Methodsmentioning

confidence: 99%

Phospholipase D-modified low density lipoprotein is taken up by macrophages at increased rate. A possible role for phosphatidic acid.

Aviram

Maor²

1993

J. Clin. Invest.

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Section: Methodsmentioning

confidence: 99%

Phospholipase D-modified low density lipoprotein is taken up by macrophages at increased rate. A possible role for phosphatidic acid.

Aviram

Maor²

1993

J. Clin. Invest.

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“…This procedure did not interfere with the modification, as analyzed by the electrophoretic mobility pattern. The electrophoretic characteristics of the lipoproteins were determined on cellulose acetate as previously described [14]. Gradient (3-12%) SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of the lipoproteins was performed under reducing conditions using ,3-mercaptoethanol.…”

Section: Patientsmentioning

confidence: 99%

Pravastatin inhibits cellular cholesterol synthesis and increases low density lipoprotein receptor activity in macrophages: in vitro and in vivo studies.

Keidar

Aviram

Maor

et al. 1994

Brit J Clinical Pharma

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1 Pravastatin, a 3-hydroxy-3-methylglutaryl coenzyme-A (HMG-CoA) inhibitor, is a highly selective inhibitor of hepatic cholesterol synthesis. We studied the in vivo and in vitro effects of pravastatin on macrophage cholesterol metabolism. 2 The effects of incubating pravastatin with human monocyte derived macrophages (HMDM), mouse peritoneal macrophages (MPM) and a J-774 A.1 macrophagelike cell line, on macrophage cholesterol synthesis, cellular degradation of native low density lipoprotein (LDL) and modified LDL, cholesterol efflux from these cells and the cholesterol esterification rate were determined. 3 Pravastatin was administered either as one 40 mg dose or 40 mg daily for 8 weeks to normocholesterolaemic and hypercholesterolaemic individuals. The effects on cholesterol synthesis and degradation in monocytes derived from these subjects were studied. 4 In vitro, pravastatin resulted in a dose-dependent inhibition of macrophage cholesterol synthesis. Cellular degradation of native LDL increased by 119% in the presence of 0.1 mg ml-' pravastatin. Degradation of both acetyl LDL and oxidized LDL was unaffected. Small concentrations of pravastatin (up to 0.19 ,ug ml-') increased the cellular cholesterol esterification rate after incubation with LDL, but higher concentrations resulted in an inhibition of the esterification. 5 Single dose pravastatin administration caused a reduction in cholesterol synthesis by the subjects own HMDM by 62% and 47% in normocholesterolaemic and hypercholesterolaemic individuals, respectively. Chronic administration resulted in a 55% inhibition of cholesterol synthesis and a 57% increase in LDL degradation. 6 The results indicate that the selective uptake of pravastatin shown for hepatocytes can be extended to macrophages. Pravastatin can inhibit cholesterol accumulation in cells of the arterial wall and by so doing exhibits an additional anti-atherogenic characteristic.

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“…A search of the literature reveals that cellulose acetate electrophoresis has been applied only to unlabeled plasma LP of fasting or fed humans or animals. Coifs and Verheyden (5) have obtained two bands: at the origin and in the a2 globulin region. These workers suggest that all the chylomicrons must move in the a2 region and that the saturation of the pores of the medium can explain the material remaining at the origin.…”

Section: Introductionmentioning

confidence: 97%

Composition, particle size and role in dietary fat transport of two different lipoproteins of the intestinal lymph

Boquillon

Paris

Clément

1972

Lipids

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Rat intestinal lymph very low density lipoproteins have been.separated by cellulose acetate eleetrophoresis. After administration of a meal including natural fats and labeled fatty acids, two labeled bands were detected on the electrophoregraphs. Further separations of these two kinds of particles were performed by density gradient zonal centrifugation. The isolated lipoproteins were analyzed for chemical composition and investigated by electron microscopy and electrophoresis. The moving particles had a higher protein and cholesterol content and a smaller diameter than the particles which remained at the origin. It has also been shown that the major part of the labeled lymph iipids were transported by the smaller particles.

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Electrophoresis and sudan black staining of lipoproteins on gelatinised cellulose acetate

Cited by 50 publications

References 3 publications

Phospholipase D-modified low density lipoprotein is taken up by macrophages at increased rate. A possible role for phosphatidic acid.

Phospholipase D-modified low density lipoprotein is taken up by macrophages at increased rate. A possible role for phosphatidic acid.

Pravastatin inhibits cellular cholesterol synthesis and increases low density lipoprotein receptor activity in macrophages: in vitro and in vivo studies.

Composition, particle size and role in dietary fat transport of two different lipoproteins of the intestinal lymph

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