2005
DOI: 10.1002/elps.200500237
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Electrophoresis in capillary cells with detection gap

Abstract: A novel design of the detection zone in multicapillary arrays used for electrophoretic separation is presented. The use of a detection gap (DG), in which the reflective surfaces separating the channels of the array are eliminated, is proposed to improve the illumination and detection of the separated DNA fragments. The electric field compression in the DG is achieved by optimization of the gap geometry. The results of the computer simulation and experiment demonstrate no substantial band-broadening in the DG. … Show more

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Cited by 2 publications
(1 citation statement)
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“…However, an exact mathematical representation of the electric field generated within each microchamber could not be established. This was due to some unknown factors, such as the parameters governing the dielectric properties, 23 the inconsistent number of induced DNA from neighbouring microchambers, as well as the deformation of the 3-dimensional PAA gel and chamber structures caused by the pressure exerted from the pushing glass needle.…”
Section: Optimization Of Experimental Parameters For Electrostretchingmentioning
confidence: 99%
“…However, an exact mathematical representation of the electric field generated within each microchamber could not be established. This was due to some unknown factors, such as the parameters governing the dielectric properties, 23 the inconsistent number of induced DNA from neighbouring microchambers, as well as the deformation of the 3-dimensional PAA gel and chamber structures caused by the pressure exerted from the pushing glass needle.…”
Section: Optimization Of Experimental Parameters For Electrostretchingmentioning
confidence: 99%