Electrophoresis of Free Sugars in Blood

Abstract

a razor blade) to expose the immature tassel for measurement. Test plants from each planting were used to estimate the stage of plant maturity for the remaining plants.Gibberellin-induced male sterility, ranging from tassels barren of all floral parts to tassels which approached normal pollen shedding, was found. Most sterile tassels developed all floral parts except stamens (pollen and pollen sacs). On partially fertile tassels the upper portion of the central spike or the terminal portions of the lateral spikes, or both, developed staminate spikelets and shed pollen. The number of anthers extruding from staminate spikelets during flowering was recorded as trace, light, moderate, and normal as a measure of relative fertility of the tassel. Silks were functional, and open-pollinated seed from male-sterile plants produced plants with fertile staminate flowers.The early flowering line planted 1 June showed no gibberellin-induced male sterility in any of the treatments, and the data are not presented. The immature tassels of this earlier flowering line, being considerably more than 1 in. in length when treated, had apparently passed the stage of development for chemical induction of male sterility.Data from field experiments are presented in Table 1. The late-flowering line planted 1 June showed sterility in varying percentages of tassels, depending on the time of application and the concentration of gibberellin applied. Application of both concentrations (500 and 1000 parts per million) of gibberellin 56 days after planting induced fewer sterile tassels on the main stalk, but it a razor blade) to expose the immature tassel for measurement. Test plants from each planting were used to estimate the stage of plant maturity for the remaining plants.Gibberellin-induced male sterility, ranging from tassels barren of all floral parts to tassels which approached normal pollen shedding, was found. Most sterile tassels developed all floral parts except stamens (pollen and pollen sacs). On partially fertile tassels the upper portion of the central spike or the terminal portions of the lateral spikes, or both, developed staminate spikelets and shed pollen. The number of anthers extruding from staminate spikelets during flowering was recorded as trace, light, moderate, and normal as a measure of relative fertility of the tassel. Silks were functional, and open-pollinated seed from male-sterile plants produced plants with fertile staminate flowers.The early flowering line planted 1 June showed no gibberellin-induced male sterility in any of the treatments, and the data are not presented. The immature tassels of this earlier flowering line, being considerably more than 1 in. in length when treated, had apparently passed the stage of development for chemical induction of male sterility.Data from field experiments are presented in Table 1. The late-flowering line planted 1 June showed sterility in varying percentages of tassels, depending on the time of application and the concentration of gibberellin applied. Application of both co...

Morphogenesis and Biochemical Processes in Schizophyllum Commune Fr.

“…Detecting reagents: Reducing substances were detected by a silver nitrate dip as described by STANGE (1959). Another general sugar reagent, which allows differentiation between hexoses and pentoses, was aniline hydrogen phthalate in 95 Ofo acetone (ROBINSON and RATHBUN, 1958). Amino-sugars were detected by the sugar reagents listed above, by ninhydrin (0.2 Ofo ninhydrin in 95 Ofo acetone), and the acetylacetone-dimethylamino-benzaldehyde reagent (PARTRIDGE, 1948).…”

A Patient With Hereditary Galactosemia Studied With a Screening Method for Galactose in Urine

“…1 paper was used and the chromatograms were run descendent for 18-20 hours. Staining was made with aniline phthalate (32). Only galactose was found in the urine samples from the patient (Fig.…”

Clinical Chemistry

“…The electrophoretic and chromatographic differentiations of blood and urinary carbohydrates were described by Date (159), Constara. (145), Coulthard and Waugh (152), Dieckert and Morris (170), and Robinson and Rathbun (524). Ek and Hultman (187) used a nexv method for blood aldosaecharide determination employing sodium paminosalicylate and acetic acid which dexnlops a pink color after heating.…”