Electrophoresis of Serum Protein with Cellulose Acetate

Briere, Russell O.; Mull, John D.

doi:10.1093/ajcp/42.5_ts.547

Cited by 53 publications

(9 citation statements)

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“…Extraspinal involvement was asymmetric at onset in 6 of the children and remained asymmetric during the course of the disease in 10. Extraspinal arthritis preceded symp toms of spinal involvement by 2-13 years in 8 patients.…”

Section: Resultsmentioning

confidence: 99%

“…T e s t s for antinuclear antibodies were performed by the indirect fluorescent method with young mouse liver as substrate ( 5 ) . Serum protein electrophoresis was done on cellulose acetate as supporting medium (6) . Serum immunoglobulins were determined by the radial diffusion method of Mancini, Carbonara, and Heremans (7) as modified by Fahey and McKelvey (8) .…”

Section: Methodsmentioning

confidence: 99%

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Juvenile ankylosing spondylitis

Ladd

Cassidy

Martel

1971

Arthritis & Rheumatism

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Fifteen patients with juvenile ankylosing spondylitis were reviewed. Eight presented with extraspinal arthritis. Certain clinical features were of value early in the disease in distinguishing these patients from those with juvenile rheumatoid arthritis: male sex, remittent oligoarthritis, late onset in childhood, relative sparing of hands and wrists, infrequent cervical spine involvement, and negative tests for rheumatoid factors and antinuclear antibodies. Roentgenographic demonstration of sacroiliac arthritis with reactive sclerosis makes the diagnosis virtually certain.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Juvenile ankylosing spondylitis

Ladd

Cassidy

Martel

1971

Arthritis & Rheumatism

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“…butyricum, unlabeled, was inoculated in heavy mineral oil (0.1 ml) into the tail of rats and, at varying times thereafter, rats were anesthetized and blood removed from the dorsal aortae into appropriate syringes for the collection of plasma and/or serum. The electrophoretic patterns [12], fibrinogens [ 13], alpha-2-glycoproteins [ 14], mucoproteins [ 15], glycoproteins [ 15], total proteins [ 16] as well as circulating white cells [17] were all determined by standard techniques. Five to 10 rats per group were used for all experiments.…”

Section: Methodsmentioning

confidence: 99%

A major contributory cause of arthritis in adjuvant-inoculated rats: Granulocytes

et al. 1977

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AbstraetThe predominant white ceil-type in circulating blood of normal rats is the lymphocyte. Granuloeytes increase in the circulating blood of adjuvant-inoculated rats; lymphocytes remain the same or increase slightly. Some drugs, like Cytosar | (cytarabine), inhibit granuioeyte increases primarily and inhibit arthritis development completely. Pretreatment of normal rats with cytarbine also inhibits the local inflammation caused by direct injections of M. butyrieum into hindpaws of rats. Pre-treatment of rats with M.butyrieum, causing granulocytes to increase in circulating blood, produces an exaggerated response to the subsequent inoculation ofM. butyricum directly into rats' paws.Injection of M. butyrieum in non-arthrltogenic vehicles (saline) does not produce the exaggerated response of granulocytes that occurs in response to injection in effective arthritogenic vehicles (mineral oil). Granulocytes are not increased greatly using the ineffective vehicle. Finally, lysates of either rat white cells (granulocytes) or platelets, both of which increase in adjuvant-inoculated rats, produce striking inflammatory reactions when inoculated locally into rats' hindpaws. The addition of non opsonized M. butyricum to granulocytes obtained from adjuvant-inoculated rats causes a significant increase in the release oflysosomal enzymes.From all these and other indirectly derived data, it is surmised that adjuvant inoculation produces a marked increase in granulocytes of circulating blood; as these increase with time, the 114. butyricum molecules or some constituent derived from them (as determined by the distribution of tritiated M. butyricum) arrive in joints and many other tissues, there reacting with granulocytes causing release of lysosomal constituents that produce the histopathology of adjuvant-disease. These results are discussed. IntroductionThe pathways and mechanisms for development of adjuvant-arthritis in the rat are unknown. Following inoculation of certain adjuvants (organism + appropriate vehicle)intradermally, rats develop a widespread disseminated disease, involving many organs -the appendages being only one manifestation of this

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“…The AHS was implemented beginning in 1958 on a cohort of 20 000 Hiroshima and Nagasaki A-bomb survivors. Celluloseacetate electrophoresis (Separax, Fujifilm, Tokyo, Japan) 3 was performed with a densitometer (Olympus AES310, Tokyo, Japan) on an aliquot of serum from each person examined in 6299 AHS participants between June 1985 and May 1995. One author (K.N.)…”

Section: Patient and Methodsmentioning

confidence: 99%

Thyroid Lymphoma of Mucosa-Associated Lymphoid Tissue with Monoclonal Gammopathy Occurring in an Atomic Bomb Survivor: Report of a Case

et al. 2000

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We describe herein a case of thyroid lymphoma of the mucosa-associated lymphoid tissue (MALT) with monoclonal gammopathy (IgG kappa), occurring in a woman who was exposed to atomic-bomb (A-bomb) radiation 1.5 km from the explosion of the hypocenter when she was 31 years old. Her estimated radiation dose was 0.2 Gy. A total thyroidectomy resulted in the disappearance of monoclonal gammopathy, thus indicating extramedullary monoclonal gammopathy of the thyroid. Three patterns of protein electrophoresis, i.e., normal, the appearance of monoclonal immunoglobulins, and their disappearance after the total thyroidectomy, were consecutively recorded. The utility of checking of the monoclonal immunoglobulin levels after a thyroidectomy due to lymphoma of the MALT to monitor recurrence is therefore suggested. This lymphoma is most likely not related to the patient's A-bomb radiation exposure.

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Electrophoresis of Serum Protein with Cellulose Acetate

Cited by 53 publications

References 0 publications

Juvenile ankylosing spondylitis

Juvenile ankylosing spondylitis

A major contributory cause of arthritis in adjuvant-inoculated rats: Granulocytes

Thyroid Lymphoma of Mucosa-Associated Lymphoid Tissue with Monoclonal Gammopathy Occurring in an Atomic Bomb Survivor: Report of a Case

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