Electrophoretic karyotype of the ascomycete Podospora anserina

Osiewacz, Heinz D.; Clairmont, Annette; Huth, Marion

doi:10.1007/bf00309921

Cited by 40 publications

(19 citation statements)

References 6 publications

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“…Using CHEF-gel electrophoresis we have separated sev- (Walz and Kt~ck 1991), Aspergillus niger (Debets et al 1990) and P. anserina (Osiewacz et al 1990). The optimized conditions for electrophoresis consist of a low field strength (40 to 50 V) combined with long running times (7 days).…”

Section: Separation O F Megabase-sized D N a From T Inflatummentioning

confidence: 99%

“…For example, several Acremonium species (Walz and Kiack 1991), Aspergillus nidulans (Brody and Carbon 1989), A. niger (Debets et al 1990) or P. anserina (Osiewacz et al 1990) possess six to eight chromosomes with a total genome size between 25 to 36 Mb. For abbreviations, see Table 1: "× 2" indicates chromosomal bands that most probably represent doublets.…”

Section: Separation O F Megabase-sized D N a From T Inflatummentioning

confidence: 99%

“…In addition, this technique has already been successfully employed for karyotype analysis of sexual as well as of asexual filamentous fungi, e.g. Aspergillus nidulans (Brody and Carbon 1989), A. niger (Debets et al 1990), Acremonium species (Skatrud and Queener 1989;Walz and Ktick 1991), Podospora anserina (Osiewacz et al 1990) or Curvularia lunata (Ridder and Osiewacz 1991).…”

Section: Introductionmentioning

confidence: 99%

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Electrophoretic karyotyping from Tolypocladium inflatum and six related strains allows differentiation of morpologically similar species

Stinberg

Walz

Schörgendorfer³

et al. 1992

Appl Microbiol Biotechnol

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Section: Separation O F Megabase-sized D N a From T Inflatummentioning

confidence: 99%

Section: Separation O F Megabase-sized D N a From T Inflatummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electrophoretic karyotyping from Tolypocladium inflatum and six related strains allows differentiation of morpologically similar species

Stinberg

Walz

Schörgendorfer³

et al. 1992

Appl Microbiol Biotechnol

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“…Separation of intact chromosomes was carried out as described by Osiewacz et al (1990) using a contour-clamped homogenous electric field (CHEF) electrophoresis system (BioRad, Richmond, CA, USA).…”

Section: Strains and Plasmids P Anserina Wild-type Strains S And A Andmentioning

confidence: 99%

Integrative transformation of the ascomycete Podospora anserina: identification of the mating-type locus on chromosome VII of electrophoretically separated chromosomes

Osiewacz

Skaletz

Esser

1991

Appl Microbiol Biotechnol

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Protoplasts of wild-type strain s and a long-lived extrachromosomal mutant (AL2) of the ascomycete Podospora anserina were transformed using a plasmid (pAN7-1) which contains the hygromycin B phosphotransferase gene (hph) of Escherichia coli under the control of Aspergillus nidulans regulatory sequences. After optimizing the transformation procedure, transformation efficiencies of 15-21 transformants/micrograms plasmid DNA were obtained. Using a second selectable vector (pBT3), which contains the beta-tubuline gene of a benomyl-resistant Neurospora crassa mutant, the co-transformation rate was determined. Southern blot hybridization experiments revealed that the transforming plasmid became integrated into the genome of the recipient either as a single copy or as multiple copies. In addition, the data from molecular as well as from classical genetic analyses indicated that in independent transformants vector integration occurred at different positions. The mitotic and meiotic stability of transformants proved to be dependent on the number of integrated plasmid copies. Genetic analyses revealed a transformant in which the integrated vector is closely linked to the mating-type locus. Fractionation of whole chromosomes by pulsed field gel electrophoresis and subsequent hybridization of the immobilized DNAs against radiolabelled vector sequences indicated the largest of seven chromosomes as the chromosome containing the integrated vector and thus the mating-type locus.

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“…In the last several years, PCD was also demonstrated to occur in multicellular fungi [2]. In the filamentous fungus Podospora anserina , a well established experimentally tractable short-lived aging model with a small genome, a role of PCD was recently demonstrated to be involved in aging and lifespan control [3-5]. Although differing in complexity, the molecular pathways controlling PCD in the different groups of organisms are conserved [6,7].…”

mentioning

confidence: 99%