Electrophoretic mobilities of human trophoblast cells and lymphocytes from pregnant women

Loke, Y.W.; Cater, D. B.; Whyte, A.; Brook, S.S.; Day, Susan

doi:10.1016/0165-0378(80)90019-4

Cited by 5 publications

(1 citation statement)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is not clear how this suppression is accomplished. Modulation of lymphocyte activity might be effected directly by trophoblasts (Loke et al 1980), by suppressor lymphocytes (Smith 1980), by altered numbers of helper lymphocytes (Sridama et al 1982), or by suppressor macrophages (Flannery et al 1983). No evidence has been found to support a role for suppressor lymphocytes in abnormal human pregnancy (Singer et al 1983).…”

Section: Commentsmentioning

confidence: 99%

Maternal Lymphocyte Responses During Normal and Abnormal Pregnancies, Measured in vitro Using Composite Trophoblast Antigens and Phytohaemagglutinin

Goodfellow

1983

Immunological Reviews

View full text Add to dashboard Cite

Section: Commentsmentioning

confidence: 99%

Maternal Lymphocyte Responses During Normal and Abnormal Pregnancies, Measured in vitro Using Composite Trophoblast Antigens and Phytohaemagglutinin

Goodfellow

1983

Immunological Reviews

View full text Add to dashboard Cite

Human Trophoblast Antigens

Loke¹

1989

Immunology of Pregnancy and Its Disorders

View full text Add to dashboard Cite

Lectin binding by microvillous membranes and coated-pit regions of human syncytial trophoblast

Whyte¹

1980

Histochem J

View full text Add to dashboard Cite

The distribution of saccharides on the microvillous membrane of the human syncytial trophoblast was investigated using ferritin conjugates of four lectins: concanavalin A (specific for alpha-D-manno- and alpha-D-glucopyranosyl residues), wheatgerm agglutinin (specific for N-acetylglucosamine), Limulus polyphemus lectin (specific for N-acetylneuraminic acid), and Lotus tetragonolobus lectin (specific for alpha-L-fucose). Concanavalin A and wheatgerm agglutinin (WGA) reacted strongly with the surface membrane and ferritin deposits were also observed in coated pit regions of the membrane. Lectins from L. polyphemus and L. tetragonolobus, however, reacted only weakly with the microvillous border and neither reacted with coated pits. Enhanced agglutinability of trophoblast cells in comparison with other foetal cells from the same conceptus was seen with WGA. This agglutination was inhibited by addition of acetylglucosamine or by a solubilized membrane fraction which was bound by a column of WGA-Sepharose. The membrane fraction which did not bind to the column did not inhibit agglutination. Electrophoresis of the WGA-bound membrane proteins revealed six subunits, the major band having an apparent mol. wt. of 55 000. A protein of this mol. wt was also seen in coated vesicles isolated from equivalent human placentae.

show abstract

Electrophoretic mobilities of human trophoblast cells and lymphocytes from pregnant women

Cited by 5 publications

References 24 publications

Maternal Lymphocyte Responses During Normal and Abnormal Pregnancies, Measured in vitro Using Composite Trophoblast Antigens and Phytohaemagglutinin

Maternal Lymphocyte Responses During Normal and Abnormal Pregnancies, Measured in vitro Using Composite Trophoblast Antigens and Phytohaemagglutinin

Human Trophoblast Antigens

Lectin binding by microvillous membranes and coated-pit regions of human syncytial trophoblast

Contact Info

Product

Resources

About