Electrophoretic Mobility of Cultured Mesodermal Tissue Cells

Abstract

NATURE1009 was obtained by centrifuging and redissolved in isotonic saline at an alkaline pH. Paper electrophoresis of this solution yielded spots with mobilities equal to those of human globulins, but no spots with the mobility of albumin. Paper electrophoresis of the supernatant of tho original precipitate resulted in a single spot which migrated with human albumin at pH 8•6 and pH 7•4.Selective precipitation has been of limited value as a preparative tool because of possible alterations of the proteins, both those precipitated and those remaining in solution. However, our rc'3ults indicate that little, if any, alterations occur when ATP is used as the precipitating agent. This may have some limited application in the separation of proteins. Furthermore, it is felt that this reaction may have a bearing on the transport and storage of high-energy phosphate compounds.

Moving Boundary Electrophoresis of Red Cells on Differently Treated Polycythaemic Patients

“…Cells in regeneration were reported to possess increased amounts of sialic acid and hence negative electric charge. This was shown for mouse liver cells after partial hepatectomy (4) and for embryonic fibroblasts in culture (5). One might assume that phlebo-in circulation in these cases no longer tomy accelerates proliferation of the erythro-originates from the abnormal clone.…”

Targeting Negative Surface Charges of Cancer Cells by Multifunctional Nanoprobes

“…In these cells, the changes of cell surface charges were driven primarily by the transient ion flows across the plasma membranes via ion pumps and ion channels without changing the immobile molecular composition. However, all these old methods, including single-cell electrodes and cell electrophoresis, were unable to evaluate the entire cell populations 6-11.…”

ZnO Nanowire Arrays Exhibit Cytotoxic Distinction to Cancer Cells with Different Surface Charge Density: Cytotoxicity is Charge‐Dependent

“…Cells were diverse in surface charge property, and cancer cells were greater negatively charged than normal cells. [26][27][28][29] Target cells in our study were supposed to exhibit suffi cient negative charge on cellular membrane and different cell lines should show distinctive charge density, otherwise the results would be insensitive for detection and analysis. Accordingly, three cancer cell lines (HepG2, Caski, and MCF-7) were selected as the target cells.…”