Electrophoretic Mobility Shift Assay Identifies Vitamin D Binding Protein (Gc-Globulin) in Human, Rat, and Mouse Sera

Tang, Weixin; Bazaraa, H.M.; Magiera, Holly; Cooke, Nancy E.; Haddad, John G.

doi:10.1006/abio.1996.0236

Cited by 6 publications

(4 citation statements)

References 11 publications

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“…They found that the urinary vitamin D was unconjugated and likely excreted along with VDBP, which has a molecular weight (VDBP – 58 kDa vs albumin 66 kDa) and isoelectric point (VDBP – variants range from pI 4.8–5.2 vs albumin – pI 4.8) similar to albumin. 17 , 18 Grymonprez et al 19 found that serum levels of VDBP were below control levels in children with NS and that serum levels of VDBP were negatively correlated with urinary excretion of VDBP. They also found that urinary VDBP (uVDBP) excretion was positively correlated with albumin excretion, but they did not distinguish between patients with SSNS and SRNS.…”

Section: Introductionmentioning

confidence: 99%

Urinary Vitamin D-Binding Protein as a Biomarker of Steroid-Resistant Nephrotic Syndrome

et al. 2016

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BACKGROUNDIdiopathic nephrotic syndrome (NS) is one of the most common glomerular disorders of childhood and is associated with increased urinary vitamin D-binding protein (uVDBP) excretion. We tested the hypothesis that uVDBP represents a biomarker to differentiate steroid-resistant nephrotic syndrome (SRNS) from the more benign forms of steroid-sensitive nephrotic syndrome (SSNS).METHODSThis cross-sectional study included children with SRNS (n = 24), SSNS (n = 28), and normal controls (n = 5). Urine and clinical data were collected from patients. Measurements of uVDBP were performed with a commercially available ELISA kit and normalized to urine creatinine.RESULTSConcentrations of uVDBP were significantly higher (P < 0.001) in patients with SRNS (13,659 ng/mL, interquartile range [IQR] 477–22,979) than in patients with SSNS (94 ng/mL, IQR 53–202) and normal controls (23 ng/mL, IQR 22–99, P = 0.002). Significance did not change when the results were corrected for urine creatinine. uVDBP was significantly negatively correlated with estimated glomerular filtration rate (eGFR; R = −0.76, P = 0.03). However, uVDBP was still markedly elevated in patients with SRNS with eGFR >100 mL/minute/1.73 m2. There was a positive correlation between microalbuminuria (MALB/Cr) and uVDBP (R = 0.67, P < 0.001). However, uVDBP displayed a much higher discriminatory ability for distinguishing SRNS than MALB/Cr (area under the curve = 0.92 vs 0.67, respectively). An uVDBP cutoff of 362 ng/mL yielded the optimal sensitivity (80%) and specificity (83%) to distinguish SRNS from SSNS.CONCLUSIONSIn this preliminary study, uVDBP represents a noninvasive biomarker that could distinguish SRNS from the more benign SSNS with high discriminatory power.

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Section: Introductionmentioning

confidence: 99%

Urinary Vitamin D-Binding Protein as a Biomarker of Steroid-Resistant Nephrotic Syndrome

et al. 2016

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“…Gc-globulin belongs to that same family as albumin and a-fetoprotein and is an a-globulin with a molecular weight of 58 kd [13]. The amount of serum Gc-globulin is 503 that of Vit-D metabolites.…”

Section: Introductionmentioning

confidence: 99%

“…Serum group-specific component globulin (Gc-globulin), also known as vitamin D-binding protein, is synthesized in the liver and has a half-life of 12 to 24 h in blood [6][7][8][9][10][11][12][13]. Gc-globulin belongs to that same family as albumin and a-fetoprotein and is an a-globulin with a molecular weight of 58 kd [13].…”

Section: Introductionmentioning

confidence: 99%

Actin-Free Gc-Globulin after Minimal Access and Conventional Anterior Lumbar Surgery

Huang

Weng

et al. 2010

Journal of Surgical Research

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“…12 Urinary vitamin D is unconjugated and is likely to be excreted with VDBP, which has a molecular weight and isoelectric point similar to albumin. 13 Therefore, low 25hydroxycholecalciferol would also contribute to poor bone health. The current study was aimed at comparing the proportional change in bone mineral content and density in children with a first episode of nephrotic syndrome on steroid therapy and evaluating the therapeutic amount for vitamin D supplementation to slow the osteoporotic changes associated with steroid therapy.…”

Section: Introductionmentioning

confidence: 99%

Four hundred IU vs One thousand IU of vitamin D supplementation in first episode of nephrotic syndrome

Lekhwani

Vaswani²,

Kumar³

et al. 2022

IJCBR

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Use of steroids in nephrotic children may lead to changes in bone mineral density and osteoporosis eventually affecting growth on a long term basis. We compared the proportionate changes in bone mineral content (BMC) and density(BMD), Vitamin D levels, Serum Calcium, phosphate and alkaline phosphatase levels in nephrotic children with the aim of giving high Vs low vitamin D doses (1000 IU Vs 400 IU) to two groups; group 1 (n=20) vs group 2 (n=20) respectively. The median BMC in group 1 increased from 11.53±3.48 g to 11.61±3.54 g after 1000 IU Vitamin D supplement and was statistically significant. However group 2 showed insignificant increases in BMC from 11.24±2.71 g to 11.25±2.67 g following 400IU Vitamin D. The change in BMD observed in group 1 from a mean of 0.426 to 0.429g/cm2 whereas in group 2 with 400 IU of vitamin D it didn’t show any significant change. The median vitamin D increased significantly in both groups; from 16.62±7.20 ng/ml to 27.45±6.47 ng/ml in group 1 while in group 2 from 18.72±8.07 ng/ml to 26.18±7.61 ng/ml which was statistically significant. The serum calcium levels normalized irrespective of 1000 IU or 400 IU of vitamin D supplementation. Changes in serum phosphate levels (decline from initial) were statistically significant however the changes in serum ALP were insignificant. We concluded that children supplemented with 1000 IU /day of vitamin D had better osteoprotection as compared to the other group.

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Electrophoretic Mobility Shift Assay Identifies Vitamin D Binding Protein (Gc-Globulin) in Human, Rat, and Mouse Sera

Cited by 6 publications

References 11 publications

Urinary Vitamin D-Binding Protein as a Biomarker of Steroid-Resistant Nephrotic Syndrome

Urinary Vitamin D-Binding Protein as a Biomarker of Steroid-Resistant Nephrotic Syndrome

Actin-Free Gc-Globulin after Minimal Access and Conventional Anterior Lumbar Surgery

Four hundred IU vs One thousand IU of vitamin D supplementation in first episode of nephrotic syndrome

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