Electrophoretic pattern of glutathione S-transferase (GST) in antibiotic resistance Gram-positive bacteria from poultry litter

Pugazhendhi, Arivalagan; Dhanarani, Sridevi; Congeevaram, Shankar; Piruthiviraj, Prakash; Ranganathan, K.; Saratale, Rijuta Ganesh; Kaliannan, Thamaraiselvi

doi:10.1016/j.micpath.2017.07.003

Cited by 20 publications

(10 citation statements)

References 37 publications

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“…The higher the concentration of pollutants entering the fish, the more the enzyme quantity, and the higher the enzyme activity [46]. When the pollutants enter the body of the tested zebrafish, the GST in liver activity decreases [47].…”

Section: Results and Analysismentioning

confidence: 99%

Biological enrichment prediction of polychlorinated biphenyls and novel molecular design based on 3D-QSAR/HQSAR associated with molecule docking

Yang

Liu

2019

Bioscience Reports

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Based on the experimental data of octanol-water partition coefficients (Kow, represents bioaccumulation) for 13 polychlorinated biphenyl (PCB) congeners, comparative molecular field analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA) were used to establish 3D-QSAR models, combined with the hologram quantitative structure–activity relationship (HQSAR), the substitution sites (mono-substituted and bis-substituted) and substituent groups (electron-withdrawing hydrophobic groups) that significantly affect the octanol-water partition coefficients values of PCBs were identified, a total of 63 monosubstituted and bis-substituted were identified. Compared with using 3D-QSAR model alone, the coupling of 3D-QSAR and HQSAR models greatly increased the number of newly designed bis-substituted molecules, and the logKow reduction in newly designed bis-substituted molecules was larger than that of monosubstituted molecules. This was established to predict the Kow values of 196 additional PCBs and carry out a modification of target molecular PCB-207 to lower its Kow (biological enrichment) significantly, simultaneously maintaining the flame retardancy and insulativity after calculation by using Gaussian09. Simultaneously, molecular docking could further screen out three more environmental friendly low biological enrichment newly designed PCB-207 molecules (5-methyl-PCB-207, 5-amino-PCB-207, and 4-amino-5-ethyl-PCB-207).

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Section: Results and Analysismentioning

confidence: 99%

Biological enrichment prediction of polychlorinated biphenyls and novel molecular design based on 3D-QSAR/HQSAR associated with molecule docking

Yang

Liu

2019

Bioscience Reports

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“…Studies have reported that in cancer cells, GST often show high levels of expression when compared to normal cells [25] and this may contribute to increase detoxi cation of anticancer drugs [26]. It have been also shown that GST Through their detoxi cation activity, might play an important role in the protection against the toxic effect of the antimicrobial agents which leads bacteria to become resistant to antibiotics [27].…”

Section: Discussionmentioning

confidence: 99%

Glutathione S-transferase M1 and T1 Genes Deletion Polymorphisms and Blood Pressure Control Among Treated Essential Hypertensive Patients in Burkina Faso.

SOMBIE

Tchélougou

Sorgho

et al. 2020

Preprint

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ObjectiveGlutathione S-transferases have been associated with experimental resistance to some drugs. The present study investigated the factors associated with blood pressure control in patients with essential hypertension, especially the role of GSTT1 and GSTM1 genes polymorphisms. This cross-sectional study in Burkina Faso consisted of 200 patients with essential hypertension and under treatment. ResultsThis cross-sectional study population consisted of 57.5 % (115/200) of patients with their hypertension under control. No statistically significant difference (p > 0.05) was found between controlled and uncontrolled (SBP and/or DBP) groups for anthropometric and biochemical parameters as well as for GSTT1 or GSTM1 gene polymorphisms. However, current alcohol consumption (OR = 3.04; CI = 1.88 - 6.13; p < 0.001), physical inactivity (OR = 3.07; CI = 1.71 - 5.49; p < 0.001), severity of hypertension (Grade III [OR = 3.79; CI = 2.00 - 7.17; p < 0.001]) and heart damage (OR = 3, 14; CI = 1.59 - 6.02; p < 0.001) were statistically more frequent in uncontrolled essential hypertension group than controlled group.

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“…GST is part of the glutathione transferase family, whose function is to catalyze nucleophilic GSH attacks on compounds containing carbon, nitrogen or sulfur, participating in detoxi cation and elimination mechanisms (Huber et al 2008). In this sense, GST plays an essential role in catalyzing the formation of adducts between antibiotics and GSH, leading to compound inactivation (Pugazhendhi et al 2017). This enzyme can also act in the detoxi cation of α, β-unsaturated compounds such as aldehydes and quinones, lipid peroxidation by-products (Huber et al 2008).…”

Section: Gst Activity and Gsh Concentrationsmentioning

confidence: 99%

Antioxidant System Alterations and Biological Health Status of Earthworms Following Long-term Exposure to Antibiotics Contaminated Poultry Litter

Sales

Silva

Araújo

et al. 2021

Preprint

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Poultry litter is widely applied as a fertilizer even though it is one of the main antibiotic sources for agricultural soils. Long-term sublethal effects (56 days) on the antioxidant system of Eisenia andrei earthworms following exposure to fluoroquinolone-contaminated poultry litter (enrofloxacin + ciprofloxacin) at 5.0, 10 and 20 g kg-1 were evaluated. The following biomarkers were assessed: superoxide dismutase (SOD), catalase (CAT), glutathione-S-trasferase (GST), reduced glutathione (GSH) and a lipid peroxidation (LPO) proxy. Significant CAT and SOD increases and a moderate positive correlation (ρ = 0.67, p < 0.05) between these enzymes was observed. Glutathione-S-trasferase levels increased significantly at 10 g kg-1, while GSH exhibited a dose-dependent response at 5.0 mg kg-1 (4 - 106%), 10 mg kg-1 (28 - 330 %) and 20 mg kg-1 (45 - 472%). LPO levels exhibited a decreasing trend with increasing poultry litter concentrations of 8 - 170% (5.0 g kg-1), 7 - 104% (10 mg kg-1) and 3 - 6% (20 mg kg-1). A Principal Component Analysis (PCA) highlighted increased SOD and CAT activities, possibly due to increases in reactive oxygen species (ROS) concentrations. Biological Health Status assessments based on the Biomarker Response Index indicate major alterations in the first month of exposure, changing to moderate in the second month. These findings indicate an antioxidant system attenuation trend. It is possible, however, that successive poultry litter applications may reduce the long-term recovery capacity of the evaluated biomarkers.

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Electrophoretic pattern of glutathione S-transferase (GST) in antibiotic resistance Gram-positive bacteria from poultry litter

Cited by 20 publications

References 37 publications

Biological enrichment prediction of polychlorinated biphenyls and novel molecular design based on 3D-QSAR/HQSAR associated with molecule docking

Biological enrichment prediction of polychlorinated biphenyls and novel molecular design based on 3D-QSAR/HQSAR associated with molecule docking

Glutathione S-transferase M1 and T1 Genes Deletion Polymorphisms and Blood Pressure Control Among Treated Essential Hypertensive Patients in Burkina Faso.

Antioxidant System Alterations and Biological Health Status of Earthworms Following Long-term Exposure to Antibiotics Contaminated Poultry Litter

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