Electrophoretic Studies of Serum Proteins of Nonpregnant and Pregnant Hamsters

Peterson, Roy P.; Turbyfill, C. L.; Soderwall, A. L.

doi:10.1016/s0015-0282(16)34634-9

Cited by 4 publications

(4 citation statements)

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“…Similar changes occur in the hamster during late gestation (Peterson, Turbyfill & Soderwall, 1962), but not in the rabbit, at least during the first third of the gestation period (Text- fig. 1 ).…”

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confidence: 52%

Disc Electrophoretic Patterns of Uteroglobin and Serum Proteins in Rabbit Blastocoelic Fluid

Hamana¹,

Hafez²

1970

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Specific proteins exist in the uterine fluid of the rabbit, but not in the maternal blood serum. These have been named`blastokinin' Krishnan & Daniel, 1967) and`u teroglobin' (Beier, 1968a). It appears on the basis of their physiochemical properties that blastokinin and uteroglobin may be identical. Blastokinin appears in uterine fluid at 3 days post coitum (p.c.) and reaches maximal concentrations at 5 days p.c., disappearing by the 9th day. It also occurs in blastocoelic fluid at 6 days p.c. (Beier, 1968b). The purpose of this investigation was to study the ontogeny of uteroglobin and serum proteins in the blastocoelic fluid of the rabbit in relation to implantation.Adult, nulliparous, New Zealand white rabbits were mated twice with two fertile bucks and injected intravenously with 20 i.u. human chorionic gonadotrophin immediately afterwards. Autopsies were performed at intervals between 5 and 10 days later. The blastocysts from does killed 5 to 6\ m=1/ 2\ days p.c. were collected by flushing the uterine horn with physiological saline. The saline was filtered off and the blastocysts were weighed and placed in a pre-cooled centrifuge tube. They were then broken up with a hypodermic needle and centrifuged at about 3000 rev/min for 10 min. The blastocoelic fluid was removed by aspiration, its volume was measured, and it was frozen for later use. At 5 to 6\ m=1/ 2\ days p.c., it was necessary to pool the blastocysts from three does in order to obtain enough blastocoelic fluid for analysis. At 7 to 10 days p.c., it was only necessary to pool those of a single doe. Maternal serum and peritoneal fluid were also collected at autopsy. The protein concentration of the blastocoelic and peritoneal fluids and of the maternal serum was determined by the method of Lowry, Rosebrough, Farr & Randall (1951), using bovine serum albumin as a standard.The procedure of Ornstein & Davis (1961) was used for disc electrophoresis of these fluids. Except for the protein-deficient fluids of early blastocysts, each column was made up to contain 0-2 mg protein. Protein-rich fluids (e.g. serum, peritoneal and blastocoelic fluids of 7 to 10 days p.c.) took up little volume and were added directly to the sample gel. However, the volume of

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confidence: 52%

Disc Electrophoretic Patterns of Uteroglobin and Serum Proteins in Rabbit Blastocoelic Fluid

Hamana¹,

Hafez²

1970

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“…The incubation mixtures were extracted as previously described (8), and the esterified and free sterol fractions were separated by silicic acid chromatography (6) . Acetone powders of hog adrenals were prepared as described by Morton( 7).…”

Section: Esterification Of Free Cholesterol and Hydrolysis Of Cholestmentioning

confidence: 99%

Esterification of Free Cholesterol and Hydrolysis of Cholesterol Oleate by Acetone Powder Extracts of Hog Adrenals.

Brot

Lossow

Chaikoff

1963

Experimental Biology and Medicine

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“…Considerable work is being carried out with the aim of producing endotoxin that would increase resistance to infections without possessing other less desirable properties, such as pyrogenicity, lethality, and ability to produce the Shwartzman phenomenon (5,6,7,8 ) . All these studies have attempted to modify endotoxin chemically.…”

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“…Normal plasma protein values covering the post-natal lifespan of the gerbil (through 28 months of age) have bzen reported by Ringle and Dellenback (4) using a bromophenol blue staining technique. In view of the importance of glycoprotein values in estimating physiologi-cal alterations and disease states ( 5,6,7,8), age-related changes in plasma glycoproteins were therefore investigated in the Mongolian gerbil. Since plasma glycoprotein values correlated with animal age have not previously been reported for the gerbil (in fact, detailed studies of this type are lacking for almost all species), the values presented here provide a set of normal glycoprotein values for future comparisons.…”

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confidence: 99%