Electrophoretically deposited carbon nanotubes as a novel support for electrogenerated silica–dehydrogenase bioelectrodes

Mazurenko, Ievgen; Etienne, Mathieu; Tananaiko, Oksana; Zaitsev, Vladimir; Walcarius, Alain

doi:10.1016/j.electacta.2012.07.068

Cited by 20 publications

(19 citation statements)

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“…Figure 2B reports the response of AuSPE‐SiO 2 ‐ChOx electrode in the absence (curve 1) and in the presence (curve 2) of choline. The deposited silica film does not influence dramatically the conductivity and the performance of the working electrode because of the small thickness and porosity which was also demonstrated earlier for other type of electrodes 34, 36, 38, 39. At the same time the peak of oxygen reduction was affected by the presence of the sol‐gel biocomposite (curve 1).…”

Section: Resultssupporting

confidence: 58%

“…The thickness and morphology of the film is controlled by changing the time and potential of electrolysis 36–38. Cathodic EAD was successfully applied to the immobilization or the co‐immobilization of various enzymes like hemoglobin, glucose oxidase, diaphorase and NAD‐dependent dehydrogenase 34, 36, 39 or living cells 40, 41. Moreover, EAD can be applied to the modification of micro‐ or nanostructured materials as it was shown with microstructured electrodes 42, Pt nanofiber assembly 43 or gold macroporous electrode 37.…”

Section: Introductionmentioning

confidence: 99%

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Amperometric Biosensor for Choline Based on Gold Screen‐Printed Electrode Modified with Electrochemically‐Deposited Silica Biocomposite

et al. 2015

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[c] 1IntroductionThed eterminationo fc holinec ontent in fooda nd biological fluids is an important analyticalt ask. Cholinei s indeed indispensable for the metabolism of mammals.I t takes part in the regulation of the insulin level and transportation of the fats in the liver as wella si nt he cell membrane construction. Cholinei ss ynthesized by the organism but additional quantities may be taken with infant food, vitamin formulasa nd sport drinks in order to avoid occurrence of deficiencyd iseases [1].M oreover, the choline determination has been reported for the diagnosis of Parkinson and Alzheimer diseases [2,3].Several methods have been developed for the choline determination, including the couplede nzymatic-spectrophotometric method [4],l iquid chromatography [ 5],e lectrochemi-and chemi-luminescence [6,7].T he actual development of personal healthc are leads to the demand for cheap and reliable sensors,e lectrochemical biosensor being one of the mostr elevant approaches [8].T he enzymatic conversion of choline to betaine aldehyde by choline oxidase (ChOx)l eads to hydrogen peroxide that can be electrochemically detected. An umber of choline electrochemical biosensors have been proposed by the immobilization of ChOx ontoe lectrode surfaces,i ncluding carbon paste electrodes [9,10],e ncapsulation in conducting [11],n onconducting[ 12] and redoxp olymers [13],i n ZnO 2 -matrix [14] using layer-by-layer assembly [3,[15][16][17] or glutaraldehyde coupling [ 2,18,19].D espite this variety of works,only afew of them have described their application as the choline determination in eggs,p asta, milk, dietetic meal, baby food [18,20] and biological fluids [10].ChOx hasa lsob eeni mmobilizedi ns ol-gelt hinf ilms in association with carbon nanotubesa nd metalnanoparticles [21,22]and showsgoodperformance forthe determination of phosphatidyl cholinei ns erum samples [ 21]. Good longterm operationals tability forimmobilized ChOx canbeattributed to theh ybrids ilicam aterials obtained by thes olgelt echnology.T hese materialsare indeed theidealm atrix fore ncapsulating biomolecules whilep reservingt heir activity [23][24][25][26][27]. In principle, thel ow-temperature sol-gel synthesisp rovidess ilicap olymerc hainsf ormation around thee nzymem oleculet hatc an keeps tericc onfiguration of thep rotein intact [26,27]. This givest he possibilityt o obtain aw ider ange of sensitivee lementso fa mperometric sensors basedonsol-gel modified electrodes.Hybrid sol-gel silica are most usually depositedo nelectrode surface by drop-coating the startings ol containing the protein(s), the silica monomer(s) and eventuallys ome additives [ 28].A nother approachh as been proposed reAbstract:Amediator-freec holine biosensor was developed using the electrochemically assisted sol-gel deposition on gold screen-printed electrodes.T he addition of 12 mM of cationic surfactant CTAB in silica sol allowed enhancing the stability of the sensor. Them odified electrode demonstrated catalytica ctivity and stable amperometric response to choline ...

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Section: Resultssupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Amperometric Biosensor for Choline Based on Gold Screen‐Printed Electrode Modified with Electrochemically‐Deposited Silica Biocomposite

et al. 2015

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“…M. Etienne et al have reported dehydrogenase-based biosensors using carbon nanotubes and sol-gel thin films on a glassy carbon electrode. 14,15 J. Tkac et al developed the hyaluronic acid dispersed carbon nanotube electrode and applied this electrode to an amperometric NADH biosensor. 16,17 However, these biosensors need improvement for their application in clinical examination in terms of sensitivity and selectivity.…”

Section: Introductionmentioning

confidence: 99%

A fiber-optic sorbitol biosensor based on NADH fluorescence detection toward rapid diagnosis of diabetic complications

Gessei

Arakawa

Kudo

et al. 2015

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Accumulation of sorbitol in the tissue is known to cause microvascular diabetic complications. In this paper, a fiber-optic biosensor for sorbitol which is used as a biomarker of diabetic complications was developed and tested. The biosensor used a sorbitol dehydrogenase from microorganisms of the genus Flavimonas with high substrate specificity and detected the fluorescence of reduced nicotinamide adenine dinucleotide (NADH) by the enzymatic reaction. An ultraviolet light emitting diode (UV-LED) was used as the excitation light source of NADH. The fluorescence of NADH was detected using a spectrometer or a photomultiplier tube (PMT). The UV-LED and the photodetector were coupled using a Y-shaped optical fiber. In the experiment, an optical fiber probe with a sorbitol dehydrogenase immobilized membrane was placed in a cuvette filled with a phosphate buffer containing the oxidized form of nicotinamide adenine dinucleotide (NAD + ). The changes in NADH fluorescence intensity were measured after adding a standard sorbitol solution. According to the experimental assessment, the calibration range of the sorbitol biosensor systems using a spectrometer and a PMT was 5.0-1000 µmol L −1 and 1.0-1000 µmol L −1 , respectively. The sorbitol biosensor system using the sorbitol dehydrogenase from microorganisms of the genus Flavimonas has high selectivity and sensitivity compared with that from sheep liver. The sorbitol biosensor allows for point-of-care testing applications or daily health care tests for diabetes patients.

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“…Модифікація ІТО/MnO2 електрода плівкою SiO2. Для покращення відтворюваності та стабільності роботи електрода, ІТО-MnO2 електрод додатково модифікували композитною плівкою SiO2, за методом електрогенерованого каталізатора, розробленим раніше [15].…”

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ITO ELECTRODES MODIFIED WITH MnO2/SiO2 FOR THE DETERMINATION OF HYDROGEN PEROXIDE

Kovalyk

Tananaiko

2018

Bull. T. Shevchenko Nat. Univ. Kyiv. Chem.

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Nanostructured transition metal oxide nanoparticles possess a catalytic activity to hydrogen peroxide. Manganese dioxide particles are one of the promising and available modificators of the electrode surface. Nanostructured MnO2 deposited onto the surface of the electrode enhances the electron transport from the H2O2 molecule to the surface. Thus selectivity and sensitivity of H2O2 detection can be improved. There are a lot of different methods of the impregnation of manganese dioxide particles onto electrode surface. An electrophoretic deposition is one of the most simple and rapid. By adjusting the electrodeposition parameters, particles of different sizes or films can be obtained. A simple and novel hydrogen peroxide sensor based on layer-by-layer assembly of MnO2 nanoparticles and SiO2 film on the ITO electrode was developed. For this purpose MnO2 nanoparticles were electrodeposited on the surface of ITO electrode from MnSO4/CH3COOK solution. The electrochemical characteristics of the modified electrodes were investigated by cyclic voltammetry.The presence of MnO2 on the surface of modified electrode was indicated by the appearance of clear oxidation-reduction peaks of Mn(IV)/Mn(III,II) at E=0.65 V in the electrolyte solution in contrast to unmodified electrode. Optimization of measurement parameters such as the amount of MnO2, applied potential and pH value were studied in details. Under the optimum conditions, the calibration curve for H2O2 determination using modified electrode was linear in the range from 1×10−4 to 1×10−3 mol/dm3 with a detection limit of 0.09×10−4 mol/dm3 (S/N = 3). The linear rang for non-modified electrode was from 1×10−3 to 1×10−2 mol/dm3 with a detection limit of 1.8×10−4 mol/dm3 (S/N = 3). The modified ITO electrode was characterized by higher current than non modified ITO as a result of increasing of electroactive surface area and catalytic effect of electrodeposited MnO2.For the stabilization of MnO2 particles and protection of the electrode surface from impurities, the ITO/MnO2 was covered by thin silica film. The selectivity of H2O2 determination at ITO modified with MnO2/SiO2 was better than at ITO especially in the presence of ascorbic acid which is oxidized at the same potential as H2O2. The oxidation current of ascorbic acid was much higher than H2O2 at nonmodified ITO in contrast to ITO modified with MnO2/SiO2. The developed ITO electrode modified with MnO2/SiO2 is a perspective element of amperometric sensor for the detection of hydrogen peroxide.

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Electrophoretically deposited carbon nanotubes as a novel support for electrogenerated silica–dehydrogenase bioelectrodes

Cited by 20 publications

References 74 publications

Amperometric Biosensor for Choline Based on Gold Screen‐Printed Electrode Modified with Electrochemically‐Deposited Silica Biocomposite

Amperometric Biosensor for Choline Based on Gold Screen‐Printed Electrode Modified with Electrochemically‐Deposited Silica Biocomposite

A fiber-optic sorbitol biosensor based on NADH fluorescence detection toward rapid diagnosis of diabetic complications

ITO ELECTRODES MODIFIED WITH MnO2/SiO2 FOR THE DETERMINATION OF HYDROGEN PEROXIDE

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