Electrophysiologic assessment of regeneration in rat sciatic nerve repair using suture, fibrin glue or a combination of both techniques

Abstract

-We evaluated the repair of seccioned rat sciatic nerve by the comparison of electro p h y s i o l o gic parameters. The repair was effected with suture (group A), fibrin glue (group B) or a combination of both techniques (group C). The amplitude, latency and conduction velocity of the motor and nerve action potentials were assessed before the nerve section and at reoperation after 24 weeks. There was no diff e rence between the groups when the nerve action potential was evaluated. Rats of group B presented better results than those of group A (p<0.05) when latency and the nerve conduction velocity assessed at the reoperation, and the ratio between the conduction velocity at the reoperation and before the nerve section in the motor action potential evaluation were measured. Animals of group C presented better re s u l t s than those of group A when the ratio between the conduction velocity of motor action potential at the reoperation and before the nerve division was considered (p<0.05). No difference between groups B and C was found. We conclude that repair using fibrin glue presented better results than suture following t r a nsection of sciatic nerve when the motor action potential was evaluated in the rat experimental model. KEY WORDS: fibrin glue, nerve regeneration, suture, sciatic rat nerve.Avaliação eletrofisiológica da eficácia de três tipos de re p a ro após a secção do nervo ciático do rato RESUMO -Foram comparados os parâmetros obtidos na avaliação eletrofisiológica do potencial de ação do nervo e do potencial de ação motor antes e após 24 semanas do re p a ro no nervo ciático do rato pre v i amente seccionado no lado direito com a utilização de sutura (grupo A), adesivo de fibrina (grupo B) ou uma combinação das duas técnicas (grupo C). Não houve diferença entre os grupos na avaliação do potencial de ação do nervo. Quando consideradas a latência e a velocidade de condução mensurados na re o p eração e a razão entre a velocidade de condução medida na reoperação e o mesmo parâmetro antes da secção do nervo, durante a mensuração do potencial de ação motor, os animais do grupo B apre s e n t a r a m melhores resultados em relação aos do grupo A (p<0,05). Os animais do grupo C apresentaram melhores resultados em comparação com os do grupo A quando considerada a razão entre a velocidade de condução medida 24 semanas do re p a ro e antes da secção do nervo durante a avaliação do potencial de ação m o t o r. Conclui-se que os animais em que o re p a ro dos nervos foi realizado com o adesivo de fibrina apresentaram melhores resultados em comparação com a sutura quando considerados os parâmetros obtidos na mensuração do potencial de ação motor. PALAVRAS-CHAVE: adesivo de fibrina, regeneração nervosa, sutura, nervo ciático do rato.

Correlation between parameters of electrophysiological, histomorphometric and sciatic functional index evaluations after rat sciatic nerve repair

“…The study was approved by the Ethics Committee of the University of São Paulo Medical School and all procedures followed national animal welfare guidelines. The data used were part of a previously reported study comparing the nerve regeneration outcome from sciatic nerve repair with suture, fibrin glue or a combination of both techniques 7 . For this study, the data were not separated according to procedure, but rather were treated as independent data points, allowing analysis of correlations between nerve regeneration parameters obtained with the use of three different techniques.…”

The new heterologous fibrin sealant in combination with low-level laser therapy (LLLT) in the repair of the buccal branch of the facial nerve

“…The clot formed by the sealant is a physiological compound found in tissue repair, and this makes it different from other types of sealants which present toxicity and high fibrin formation [17]. Fibrin sealant presents its own biological properties, being versatile in different clinical settings, including in combination with conventional suture techniques [16,18].…”

Estudio anatómico de la transferencia de los nervios accesorio y toracodorsal al nervio cubital en el gato

“…La aparición de los geles de fibrina ha supuesto una revolución en la sutura nerviosa puesto que carecen de efecto inhibitorio sobre la regeneración axonal (36), alcanzando resultados similares en menos tiempo (37,38), hasta el punto de que consiguen menor fibrosis que las suturas, mejor regeneración axonal (39) y mayor velocidad de conducción y amplitud de onda en los estudios neurofisiológicos (40). Por estas razones utilizamos la sutura epineural con nylon 10-0 de aproximación, junto con el sellado de la anastomosis mediante gel de fibrina en todos los casos.…”