Electrophysiological Activity and Survival Rate of Rats Nervous Tissue Cells Depends on D/H Isotopic Composition of Medium

Abstract

The deuterium content modification in an organism has a neuroprotective effect during the hypoxia model, affecting anxiety, memory and stress resistance. The aim of this work was to elucidate the possible mechanisms of the medium D/H composition modification on nerve cells. We studied the effect of an incubation medium with a 50 ppm deuterium content compared to a medium with 150 ppm on: (1) the activity of Wistar rats’ hippocampus CA1 field neurons, (2) the level of cultured cerebellar neuron death during glucose deprivation and temperature stress, (3) mitochondrial membrane potential (MMP) and the generation of reactive oxygen species in cultures of cerebellar neurons. The results of the analysis showed that the incubation of hippocampal sections in a medium with a 50 ppm deuterium reduced the amplitude of the pop-spike. The restoration of neuron activity was observed when sections were returned to the incubation medium with a 150 ppm deuterium content. An environment with a 50 ppm deuterium did not significantly affect the level of reactive oxygen species in neuron cultures, while MMP decreased by 16–20%. In experiments with glucose deprivation and temperature stress, the medium with 50 ppm increased the death of neurons. Thus, a short exposure of nerve cells in the medium with 50 ppm deuterium acts as an additional stressful factor, which is possibly associated with the violation of the cell energy balance. The decrease in the mitochondrial membrane potential, which is known to be associated with ATP synthesis, indicates that this effect may be associated with the cell energy imbalance. The decrease in the activity of the CA1 field hippocampal neurons may reflect reversible adaptive changes in the operation of fast-reacting ion channels.

Study of the Magnesium Comenate Structure, Its Neuroprotective and Stress-Protective Activity

“…The brain is the most sensitive organ to the disturbance of redox homeostasis [4][5][6][7]. This is due to the following factors.…”

Isotope effect of 13C‑enriched testosterone on human cells

“…For decades, various efforts have been made to explore the biology isotope effects of biogenic elements. Despite the increasing knowledge of the biological isotope effect, a comprehensive understanding of the effects in the biological context has not yet been achieved (2,(5)(6)(7)(8)(9). Among the main biogenic elements (C, H, O and N), deuterium has been investigated in a number of studies and its effects on organisms have been demonstrated (5)(6)(7)(8)(9).…”

“…Despite the increasing knowledge of the biological isotope effect, a comprehensive understanding of the effects in the biological context has not yet been achieved (2,(5)(6)(7)(8)(9). Among the main biogenic elements (C, H, O and N), deuterium has been investigated in a number of studies and its effects on organisms have been demonstrated (5)(6)(7)(8)(9). However, the carbon isotope effects of bioactive compounds have yet not been investigated, at least to the best of our knowledge, although such studies are important to the biology development in theory and application.…”

Reduction of Deuterium Level Supports Resistance of Neurons to Glucose Deprivation and Hypoxia: Study in Cultures of Neurons and on Animals

“…At the same time, even relatively small increases or decreases in its concentration affect physiological processes at all levels: molecular, cellular, tissue, organ and organism [ 6 , 7 , 8 , 9 , 10 ]. Many studies have shown that a decrease in the deuterium content supports the body’s defense systems, including nonspecific mechanisms [ 11 , 12 , 13 , 14 ].…”