Electrophysiological analysis of the HeNe laser effects at the mouse neuromuscular junction

Effect of low power 655 nm diode laser irradiation on the neuromuscular junctions of the mouse diaphragm

“…In our study, diode laser in the 655 nm and radiant exposure of 1, 2, 4 and 12 J/cm 2 did not affect evoked or spontaneous neuromuscular transmission. The results of Re et al [51], who considered the evoked release, were similar. These authors used a similar wavelength (HeNe laser, 632.8 nm) in an in vitro model, but with dose of laser irradiation (30 J/cm À2 ) that are not usually used in clinical treatments, and found only a small change in the frequency and decay time of the spontaneous postsynaptic currents.…”

“…Although this mechanism is probably related to intracellular calcium movements, it is different from the evoked release mechanism [52]. The experiments of Re et al [51], were performed in low calcium-high magnesium media in order to prevent the muscle cells from twitching. Because transmembrane calcium fluxes, as well as the hypothesised calcium release from internal stores, may be involved in a possible LLLT effect, we recorded EPPs also in dTC postsynaptically blocked preparations.…”