Electrophysiological and electron‐microscopical correlations with fluid and electrolyte secretion in rabbit ileum.

Holman, Geoffrey D.; Naftalin, Richard J; Simmons, Nicholas L.; Walker, Muriel H.

doi:10.1113/jphysiol.1979.sp012776

Cited by 37 publications

(16 citation statements)

References 36 publications

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“…D-Ala2-Met E may also inhibit Cl permeability at the brush border which would result in a decrease in S to NI Cl movement, a decrease in the ISc and an increase in net Cl absorption. Cyclic AMP, which has the opposite effect of D-Ala2-Met E in the rabbit ileum, may work primarily by increasing the permeability of the brush border membrane to Cl (22,(25)(26)(27). Thus it is not unreasonable to postulate that an agent that stimulates absorption may do so by decreasing Cl permeability at the brush border.…”

Section: Discussionmentioning

confidence: 99%

“…Cyclic AMP (a) stimulates electrogenic chloride secretion; (b) inhibits neutral NaCl influx; (c) increases the permselectivity ofthe shunt pathway and/or permeability of the brush border to chloride (25)(26)(27)(28)(29)(30). Enkephalins could thus: (a) be inhibiting the production of cyclic AMP; (b) blocking the effect of cyclic AMP on ion transport; or (c) working independently ofthe cyclic nucleotides, but having the opposite effect.…”

Section: Discussionmentioning

confidence: 99%

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Effect of D-alanine methionine enkephalin amide on ion transport in rabbit ileum.

Racusen²,

1980

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A B S T R A C T The presence of enkephalins in the intestine and the use of opiates to treat diarrheal diseases suggests that enkephalins may affect intestinal ion transport. Using isolated rabbit ileal mucosa, we found that leucine enkephalin, methionine enkephalin, and D-Ala2-methionine enkephalin amide (D-Ala2-Met E) decreased the short circuit current (ISC) and potential difference although the effect ofD-Ala2-Met E was more pronounced and prolonged. D-Ala2-Met E increased net sodium (+1.27±0.5 pueq/cm2h), and chloride absorption (+2.33±0.4), and increased tissue conductance by 37%. Although the effect of enkephalin on ion transport is opposite that ofcyclic AMP, D-Ala2-Met E had no effect on basal or vasoactive intestinal polypeptide-stimulated cyclic AMP levels. The effect of D-Ala2-Met E on ISC was blocked by naloxone, suggesting the involvement of specific opiate receptors.Tetrodotoxin completely blocked the decrease in ISC induced by D-Ala2-Met E but not by epinephrine, inferring that enkephalins are preganglionic neurotransmitters. The effect of D-Ala2-Met E on ISC was not blocked by phentolamine, haloperidol, or pretreatment of animals with 6-hydroxydopamine, suggesting that enkephalin does not affect the Isc by stimulating the release of a-adrenergic or dopaminergic agonists. D-Ala2-Met E also decreased the Is, in the presence of carbachol and bethanechol, indicating that enkephalin does not inhibit the release of acetylcholine. Further, up to 10 ,uM atropine had no effect on the ISC. These studies demonstrate that enkephalins stimulate intestinal ion transport and may do so by stimulating (or inhibiting) the release of a nonadrenergic, noncholinergic neurotransmitter.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of D-alanine methionine enkephalin amide on ion transport in rabbit ileum.

Racusen²,

1980

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“…However, secretion usually goes together with an increased transepithelial resistance. Apparently, this is not because of a decrease of the Na + conductance in the tight junctions but is caused by the decrease of the diameter of the lateral intercellular spaces [8,10,18,27]. We propose that the interspace collapses because of the depletion of NaCl caused by a faster uptake through the basolateral membrane than diffusion of NaCl into the interspace, as discussed in the next paragraph.…”

Section: The Modulation Of the CL − Conductance Of The Tight Junctionmentioning

confidence: 80%

“…Both, the opening of apical Cl − and basolateral K + channels and a possible increase of Cl − permeability of the tight junctions would decrease the transepithelial resistance. Hence, the observed increase is, most probably, due to a collapse of the lateral intercellular spaces [8,10,18,27]. The collapse may result from the net loss of salt and water from the lateral intercellular spaces via the cells to the luminal side.…”

Section: Effect Of Passage Of Transepithelial Currentmentioning

confidence: 95%

“…8, namely that application of cAMP to specimen with the lower initial resistances decreased the transepithelial conductance, suggests that the Na + conductance and hence the cation selectivity of the tight junctions is still too high to allow sufficient Cl − recycling-despite the increase of the Cl − conductance in tight junctions-and to prevent a collapse of the lateral spaces. Further evidence for the role of the Na + conductance of the tight junctions in the collapse of the interspace can be drawn from Holman et al [18]. These authors have shown that theophylline induces a collapse of the intercellular space in rabbit ileum.…”

Section: Variation In Behavior Of Individual Tissue Preparationsmentioning

confidence: 98%

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The Chloride Conductance of Tight Junctions of Rat Ileum Can Be Increased by cAMP But Not by Carbachol

Bijlsma

Bakker

Groot

1997

Journal of Membrane Biology

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The chloride conductance of tight junctions of rat ileum can be increased by cAMP but not by carbachol Bijlsma, P.B.; Bakker, R.C. Published in:The journal of membrane biology DOI:10.1007/s002329900222Link to publication Citation for published version (APA):Bijlsma, P. B., & Bakker, R. C. (1997). The chloride conductance of tight junctions of rat ileum can be increased by cAMP but not by carbachol. The journal of membrane biology, 157(2), 127-137. DOI: 10.1007/s002329900222 General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Abstract. It is well known, that in mammalian small intestine, cAMP increases Cl − permeability of the apical membrane of enterocytes as part of its secretory action. Paradoxically, this is usually accompanied by an increase of the transepithelial resistance. In the present study we report that in the presence of bumetanide (to block basolateral Cl − uptake) cAMP always decreased the transepithelial resistance. We examined whether this decrease in resistance was due to a cAMP-dependent increase of the paracellular electrolyte permeability in addition to the increase of the Cl − permeability of the apical cell membrane. We used diffusion potentials induced by serosal replacement of NaCl, and transepithelial current passage to evoke transport number effects. The results revealed that cAMP (but not carbachol) could increase the Cl − permeability of the tight junctions in rat ileum. Moreover, we observed a variation in transepithelial resistance of individual tissue preparations, inversely related to the cation selectivity of the tissue, suggesting that Na + permeability of the tight junctions can vary between preparations.

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Long‐term effects of tumor necrosis factor on LLC‐PK₁ transepithelial resistance

Marano

Laughlin

Russo

et al. 1993

Journal Cellular Physiology

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Renal epithelial LLC-PK1 cell sheets incubated with tumor necrosis factor (TNF) undergo an acute, spontaneous, and rapidly reversible decrease in transepithelial resistance (TER). (Mullin et al., 1992). However, 24 to 72 h following TNF exposure, TER across the cell sheet increases 2-fold. This later effect of TNF is also reversible, albeit slowly. The TER of TNF-treated cell sheets then declines toward initial levels between 72 and 144 h following exposure to the cytokine. Whereas the long-term increase in TER following TNF exposure is not associated with a decreased transepithelial 14C-mannitol flux (size selectivity), the charge (anionic) selectivity of the LLC-PK1 tight junction is decreased. Basal-lateral (ouabain and bumetanide-insensitive) Rb+ and apical Na+-dependent alpha-methylglucoside (AMG) uptake into the cell are both reduced in cultures exposed to TNF 24 h earlier. Correspondingly, this long-term effect on TER is accompanied by a 30% decrease in short circuit current (iscc). Along with an observed increase in basal-lateral methylamino-isobutyric acid (MeAIB) influx into the cells, an increased incorporation of [3H]-thymidine into DNA indicates increased cell cycling after exposure to TNF. While the increase in cell cycling is not sustained for the duration of the elevation in TER, it does appear to initiate a sequence of events that lead to the sustained increase in TER. A decrease in the lateral intercellular space, observed between these epithelial cells after long-term TNF exposure, may be a mechanism for the elevated TER following from the mitogenesis and/or transport changes. This overall long-term tightening of an epithelium in response to TNF may function, in part, as a compensatory action of the epithelium to reestablish its effectiveness as a physiological barrier, following the acute effect of TNF.

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Electrophysiological and electron‐microscopical correlations with fluid and electrolyte secretion in rabbit ileum.

Cited by 37 publications

References 36 publications

Effect of D-alanine methionine enkephalin amide on ion transport in rabbit ileum.

Effect of D-alanine methionine enkephalin amide on ion transport in rabbit ileum.

The Chloride Conductance of Tight Junctions of Rat Ileum Can Be Increased by cAMP But Not by Carbachol

Long‐term effects of tumor necrosis factor on LLC‐PK₁ transepithelial resistance

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Electrophysiological and electron‐microscopical correlations with fluid and electrolyte secretion in rabbit ileum.

Cited by 37 publications

References 36 publications

Effect of D-alanine methionine enkephalin amide on ion transport in rabbit ileum.

Effect of D-alanine methionine enkephalin amide on ion transport in rabbit ileum.

The Chloride Conductance of Tight Junctions of Rat Ileum Can Be Increased by cAMP But Not by Carbachol

Long‐term effects of tumor necrosis factor on LLC‐PK1 transepithelial resistance

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Long‐term effects of tumor necrosis factor on LLC‐PK₁ transepithelial resistance