Electrophysiological and functional evaluation of peroneal nerve regeneration in rabbit following topical hyaluronic acid or tacrolimus application after nerve repair

Abstract

Electrophysiological analyses and functional evaluations based on the TSI indicate that HA and FK-506 exert similar, positive effects on nerve regeneration that are superior to those observed in response to saline treatment.

The Role of Pharmacological Agents in Nerve Regeneration after Peripheral Nerve Repair

“…Recently, we published two original articles that compare the effects of HA and FK-506 on nerve regeneration. We found that our electrophysiological and biomechanical measurements as well as our results of functional evaluations indirectly indicate that the effects of HA and FK506 on nerve regeneration are similar [98,99].…”

“…According to our results, HA and FK506 appear to have similar effects (P > 0.05) with respect to preventing scar formation and improving nerve regeneration compared with saline (P < 0.05) [80,98]. However, it should be mentioned that we observed no significant differences in biomechanical properties in the HA and FK506 groups compared to the saline group (P > 0.05) [99].…”

“…Methylprednisolone Neuroprotective role By its inhibition of CD3-positive inflammatory cell infiltration in local tissue and consequently recruitment of macrophages Feng and Yuan [123] Anti-inflammatory effects Through inhibition of oxygen-free radical-induced lipid peroxidation Hall [124] Vitamin B12 Nerve regeneration Owing to axoplasm flow within the neuraxon, in order to normalise the neuraxon's skeleton protein transportation as well as accelerating the formation of the myelin sheath Wang et al [129] Neuroprotectant Through increasing the number of Schwann cells and myelinated nerve fibres, and the diameter of axons Lopatina et al [132] Through antioxidant properties, because it is also a good scavenger of reactive oxygen species tion of the effects of these pharmacological agents in the prevention of scar formation and nerve regeneration in experimental animals is performed by electrophysiological measurements and through assessing functional recovery, whereas after sacrificing the animals, other methods have been used, such as macroscopic, histomorphometric, immunohistochemical and biomechanical techniques [70,91,98,99]. Ozgenel found that nerves treated with HA have a significant reduction in perineural thickness compared to nerves treated with just saline (P < 0.05).…”

The neurochemistry of peripheral nerve regeneration

“…Electrophysiological studies and functional evaluation (toe-spreading index) revealed similar results for FK506 and HA. [70] Zor et al investigated the effect of HA (film sheath at the site of neurorrhaphy), VEGF (VEGF gene in plasmid injection) and combined therapy, on axonal regeneration. The group subject to combined therapy had well myelinated, compact fibres aligned with SCs.…”

A Rabbit Model for Peripheral Nerve Reconstruction Studies Avoiding Automutilation Behavior

“…We have conducted a review of 40 reports in which a rabbit animal model was used for evaluation of nerve regeneration. 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 To date, the sciatic nerve injury and repair model is the most well-documented nerve injury model in rabbits with 45% of studies performed using the sciatic nerve. In a study by Hsu et al, 19 a 2-cm section of the sciatic nerve was removed after making an incision from the greater trochanter to the mi-calf and splitting the muscle to expose the sciatic nerve.…”