Electrophysiological properties of human adipose tissue-derived stem cells

Abstract

Human adipose tissue-derived stem cells (hASCs) represent a potentially valuable cell source for clinical therapeutic applications. The present study was designed to investigate properties of ionic channel currents present in undifferentiated hASCs and their impact on hASCs proliferation. The functional ion channels in hASCs were analyzed by whole-cell patch-clamp recording and their mRNA expression levels detected by RT-PCR. Four types of ion channels were found to be present in hASCs: most of the hASCs (73%) showed a delayed rectifier-like K(+) current (I(KDR)); Ca(2+)-activated K(+) current (I(KCa)) was detected in examined cells; a transient outward K(+) current (I(to)) was recorded in 19% of the cells; a small percentage of cells (8%) displayed a TTX-sensitive transient inward sodium current (I(Na.TTX)). RT-PCR results confirmed the presence of ion channels at the mRNA level: Kv1.1, Kv2.1, Kv1.5, Kv7.3, Kv11.1, and hEAG1, possibly encoding I(KDR); MaxiK, KCNN3, and KCNN4 for I(KCa); Kv1.4, Kv4.1, Kv4.2, and Kv4.3 for I(to) and hNE-Na for I(Na.TTX). The I(KDR) was inhibited by tetraethyl ammonium (TEA) and 4-aminopyridine (4-AP), which significantly reduced the proliferation of hASCs in a dose-dependent manner (P < 0.05), as suggested by bromodeoxyurindine (BrdU) incorporation. Other selective potassium channel blockers, including linopiridine, iberiotoxin, clotrimazole, and apamin also significantly inhibited I(KDR). TTX completely abolished I(Na.TTX). This study demonstrates for the first time that multiple functional ion channel currents such as I(KDR), I(KCa), I(to), and I(Na.TTX) are present in undifferentiated hASCs and their potential physiological function in these cells as a basic understanding for future in vitro experiments and in vivo clinical investigations.

Generation and characterization of human cardiac resident and non-resident mesenchymal stem cell

“…Many subsequent studies have demonstrated that, umbilical cord and heart derived MSCs expressed identical levels of surface antigens such as CD90, CD44, CD105 and CD73. The present immunophenotyping pattern of MSCs derived from umbilical cord and human heart were found to be consistent with the reports of other groups (Hoogduijn et al 2007;Park et al 2007;Bai et al 2007). In addition, ) between experimental groups.…”

“…As well, the presence of Na? channel is responsible for inward sodium current (Bai et al 2007;Park et al 2007). …”

“…current (I to ) and TTX-sensitive transient inward sodium current (I Na.fTTX ) are widely expressed in different types of cells to regulate the physiobiochemical reactions as they play a fundamental role in maintaining the physiological homeostasis, cell proliferation, and signal transduction at different cell stages (Bai et al 2007;Chen et al 2007;Nilius and Droogmans 2001). Unlike other mature cells, cardiac cells are rigidly controlled cells that produce and conduct impulse to mediate synchronized contraction for uninterrupted myocardial function.…”

“…Currently there are only few reports available on the electrophysiological properties of cardiac resident and non-resident MSCs (Bai et al 2007;Chen et al 2007;Nilius and Droogmans 2001). Hence, the present study was designed to investigate the electrophysiological properties of human umbilical cordderived mesenchymal stem cell as a non-resident stem cell and compared with the resident stem cell of cardiac-derived mesenchymal stem cells.…”

Human adipose-derived stem cells for the treatment of intracerebral hemorrhage in rats via femoral intravenous injection

“…Brain edema formation and BBB (blood-brain barrier) disruption, caused by both hematomal and peri-hematomal injuries, have also been reported to be an important component of brain injury after ICH [7][8][9][10][11][12]. Mesenchymal stem cells generated from murine or human adipose tissues have been well documented [13][14][15][16][17][18][19][20][21][22] and have been named as adipose-derived stem cells [14,15,19,20], adipose-derived adult stem cells [21], adipose derived stromal cells [16], adipose derived mesenchymal stem cells [18], adipose tissuederived stem cells [13], and adipose tissue stromal cells [17]. Systemic transplantation of human adipose-derived stem cells to attenuate cerebral inflammation and to facilitate motion recovery of rats from cerebral degeneration induced by ICH has been attempted [23].…”