Electrophysiological recordings of patterned rat brain stem slice neurons

Combined topographical and chemical micropatterns for templating neuronal networks

“…Two-dimensional neuronal networks grown on patterned templates have been investigated previously [6][7][8][9][10][11][12][13][23][24][25]. Yet, there are significant challenges associated with these patterning schemes.…”

Synaptic plasticity in micropatterned neuronal networks

“…One of the first examples of neuronal patterning was reported by Kleinfeld et al in 1988 [8], who used photolithography to pattern different types of amines for neuronal attachment onto a background modified with cell-repellent alkane chains. Later on, a number of photochemical methods [9][10][11][12], laser ablation of self-assembled monolayers [13] and microcontact printing [14][15][16][17] were added as tools to create micronscale patterns of adhesive molecules against a nonadhesive background.…”

Impact of micropatterned surfaces on neuronal polarity

“…A number of molecules have been used successfully for patterning, including the physiological adhesion molecule laminin (Yeung et al, 2001) or fragments thereof (Scholl et al, 2000), synthetic molecules like polylysine (Corey et al, 1991) or various types of amines (Kleinfeld et al, 1988;Ma et al, 1998;Schaffner et al, 1995). As a cell repellent background, a range of hydrophobic materials has been applied, such as polyethylene glycol (Wheeler et al, 1999), polystyrene (Lauer et al, 2002) or fluorinated silane groups .…”