Electrophysiological recordings of single ion channels in planar lipid bilayers using a polymethyl methacrylate microfluidic chip

Suzuki, Hiroaki; Tabata, Kazuhito V.; Noji, Hiroyuki; Takeuchi, Shoji

doi:10.1016/j.bios.2006.04.013

Cited by 60 publications

(52 citation statements)

References 19 publications

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“…Furthermore, hydrogel solutions containing gramicidin were extremely stable and could be used months after preparation for bilayer experiments. In future work, we will report on the progress of this artificial bilayer technique including our goal to automate the procedure by applying laboratory chip technology that will allow for a more rapid, voluminous method [10].…”

Section: Discussionmentioning

confidence: 99%

Lipid Bilayers at Gel/Gel Interface for Ion Channel Recordings

Hirano

Kobayashi²,

Ide

2008

e-J. Surf. Sci. Nanotechnol.

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We have developed a practical method to produce durable artificial lipid bilayers using a hydrogel-hydrogel interface for ion channel measurements. Bilayers were formed by forcing a hydrogel-bead into contact with the hydrogel layer (hydrogel plate) in a lipid solution. The immediate formation of a bilayer was observed (< 1 s). This allows channel recordings to be repeated more easily and quickly as compared to conventional methods. Currents of various types of channel such as gramicidin, hemolysin and BK-channel have been recorded. Our channel property results mirrored those of other techniques and were reproducible. Hydrogel solutions containing gramicidin were extremely stable and could be used months after preparation for bilayer experiments.

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Section: Discussionmentioning

confidence: 99%

Lipid Bilayers at Gel/Gel Interface for Ion Channel Recordings

Hirano

Kobayashi²,

Ide

2008

e-J. Surf. Sci. Nanotechnol.

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“…Upon alamethicin and ␣-HL insertion into bilayers, 60 signals with a high S/N ratio have been recorded at a clamped voltage of 60 and 40 mV, respectively. 61 This experiment demonstrates that the mentioned three tasks can be performed by a relatively simple microfluidic system buildup of the commonly used material polymethylmethacrylate ͑PMMA͒.…”

Section: Discussionmentioning

confidence: 77%

“…Such automatic microfluidic systems have been developed for biosensors in general and recently also to generate lipid bilayers. [58][59][60][61][62] The formation of bilayers can be achieved by a controlled pumping of a low concentration of the lipid dissolved in decane to the cis-side whereas the trans-side is filled with electrolyte. Upon alamethicin and ␣-HL insertion into bilayers, 60 signals with a high S/N ratio have been recorded at a clamped voltage of 60 and 40 mV, respectively.…”

Section: Discussionmentioning

confidence: 99%

Nano for bio: Nanopore arrays for stable and functional lipid bilayer membranes (Mini Review)

Tiefenauer¹,

Studer²

2008

Biointerphases

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“…Shiu et al measured the lengths of stretched DNA molecules in the ordered in-channel nanopores [96]. The channel current through membrane-bound ion channels on reconstituted lipid bilayer was recorded by Suzuki et al [97,98] and Hromada et al [99]. This technique can be used for studying electrophysiology at the singlemolecule level.…”

Section: Smd In Nanofluidic Devicesmentioning

confidence: 97%

Recent advances in single‐molecule detection on micro‐ and nano‐fluidic devices

Liu¹,

Qu²,

Luo³

et al. 2011

Electrophoresis

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Single-molecule detection (SMD) allows static and dynamic heterogeneities from seemingly equal molecules to be revealed in the studies of molecular structures and intra- and inter-molecular interactions. Micro- and nanometer-sized structures, including channels, chambers, droplets, etc., in microfluidic and nanofluidic devices allow diffusion-controlled reactions to be accelerated and provide high signal-to-noise ratio for optical signals. These two active research frontiers have been combined to provide unprecedented capabilities for chemical and biological studies. This review summarizes the advances of SMD performed on microfluidic and nanofluidic devices published in the past five years. The latest developments on optical SMD methods, microfluidic SMD platforms, and on-chip SMD applications are discussed herein and future development directions are also envisioned.

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Electrophysiological recordings of single ion channels in planar lipid bilayers using a polymethyl methacrylate microfluidic chip

Cited by 60 publications

References 19 publications

Lipid Bilayers at Gel/Gel Interface for Ion Channel Recordings

Lipid Bilayers at Gel/Gel Interface for Ion Channel Recordings

Nano for bio: Nanopore arrays for stable and functional lipid bilayer membranes (Mini Review)

Recent advances in single‐molecule detection on micro‐ and nano‐fluidic devices

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