Electrophysiological studies of the action of gonadotropins on Leydig and Sertoli cells from rat testis

Joffre, M.; Roche, A.; Duchatelle, Pascal

doi:10.1051/rnd:19880703

Cited by 5 publications

(1 citation statement)

References 15 publications

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“…c-Kit signaling is known to be associated with steroidogenesis in Leydig cells (Rothschild et al, 2003). In mice with reduced c-Kit expression, spermatogenesis is blocked after birth (Ye et al, 2017) Leydig cells isolated from testis show K + and Clcurrents evoked by depolarizing voltage steps (Joffre et al, 1988), indicating that Leydig cells may have electrical properties.…”

Section: Discussionmentioning

confidence: 99%

Testosterone secretion is affected by receptor tyrosine kinase c-Kit and anoctamin 1 activation in mouse Leydig cells

Woo

Kang

2022

J Anim Reprod Biotechnol

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Receptor tyrosine kinase c-Kit, a marker found on interstitial cells of Cajal (ICCs), is expressed in Leydig cells, which are testicular interstitial cells. The expression of other ICC markers has not yet been reported. In this study, we investigated the expression of c-Kit and anoctamin 1 (ANO1), another ICC marker, in mouse testes. In addition, the relationship between c-Kit and ANO1 expression and Leydig cell function was investigated. We observed that c-Kit and ANO1 were predominantly expressed in mouse Leydig cells. The mRNA and protein of c-Kit and ANO1 were expressed in TM3, a mouse Leydig cell line. LH induced an increase in intracellular Ca 2+ concentration, membrane depolarization, and testosterone secretion, whereas these signals were inhibited in the presence of c-Kit and ANO1 inhibitors. These results show that c-Kit and ANO1 are expressed in Leydig cells and are involved in testosterone secretion. Our findings suggest that Leydig cells may act as ICCs in testosterone secretion.

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Section: Discussionmentioning

confidence: 99%

Testosterone secretion is affected by receptor tyrosine kinase c-Kit and anoctamin 1 activation in mouse Leydig cells

Woo

Kang

2022

J Anim Reprod Biotechnol

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Granulosa cells have calcium-dependent action potentials and a calcium-dependent chloride conductance

et al. 1994

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We have found chicken granulosa cells to be excitable. Experiments using the whole-cell patch-clamp technique showed that they had membrane resting potentials of -62 +/- 3 mV (n = 8) and generated action potentials, either in response to 10-ms depolarizing current pulses or, on occasion, spontaneously. The action potentials persisted in a Na(+)-free bath and were reversibly blocked by 4 mM Co2+. They lasted 0.9-3.0s with 64 mM Cl- in the pipette, were shortened 67 +/- 8% by the Cl- channel blocker 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB; 20 microM), and lengthened to 8.7 +/- 2.2 when the Cl- equilibrium potential (Vcl) was changed from -20 mV to -2 mV by using 134 mM Cl- in the pipette. With conventional whole-cell voltage-clamp, slowly activating and inactivating currents, which reached maximum amplitude after 0.35-1.40 s, were evoked by depolarizing voltage steps. These slow currents activated between voltage steps of -60 mV and -50 mV and reached a maximum inward amplitude at about -40 mV. Changing the Cl- concentration in the pipette (VCl of -2MV or -20 mV) or bath (VCl of -2 mV or + 18 mV) shifted their reversal potential in a direction consistent with a Cl- electrode. They were inhibited by the Cl- channel antagonists 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS; 0.5 mM), NPPB (20 microM), and 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid (SITS; 0.5 mM). The slow currents were blocked by Ca2+ deprivation, or by CO2+ (4 mM), or by replacing external Ca2+ with Ba2+.(ABSTRACT TRUNCATED AT 250 WORDS)

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A Spermatid Factor Inhibits cAMP and Calcium Signaling in Sertoli but Not Leydig Cells

Morris

Hodgskin

Fujisawa

1994

Proceedings of the 1992 Laurentian Hormone Conference

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Electrophysiological studies of the action of gonadotropins on Leydig and Sertoli cells from rat testis

Abstract: These results suggest that hCG stimulation involved the blockade of a potassium current and the activation of a chloride current through an increase of intracellular calcium.Introduction.

Cited by 5 publications

References 15 publications

Testosterone secretion is affected by receptor tyrosine kinase c-Kit and anoctamin 1 activation in mouse Leydig cells

Testosterone secretion is affected by receptor tyrosine kinase c-Kit and anoctamin 1 activation in mouse Leydig cells

Granulosa cells have calcium-dependent action potentials and a calcium-dependent chloride conductance

A Spermatid Factor Inhibits cAMP and Calcium Signaling in Sertoli but Not Leydig Cells

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