1984
DOI: 10.1007/bf01868689
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Electrophysiology of a clonal osteoblast-like cell line: Evidence for the existence of a Ca2+-activated K+ conductance

Abstract: Intracellular microelectrode measurements were made on a well-characterized osteoblast-like clonal cell line isolated from a rat osteosarcoma. In serum-free medium, stable membrane potentials of -42 +/- 9 mV (SD, n = 190) were recorded. Ion substitution experiments suggested that this membrane potential is primarily a Na+/K+ diffusion potential. Input resistance was correlated strongly with colony size, ranging from 49 +/- 18 M omega (SD, n = 14) for colonies of 1-3 cells, to 4 +/- 4 M omega (SD, n = 164) for … Show more

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Cited by 43 publications
(19 citation statements)
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“…An increase in Ca2+ conductance with a n increase in internal cAMP has been shown to occur in other cell types (Tsien, 1983;Cachelin et al, 1983;Rasmussen and Barrett, 1984). Previous work on RCJ 1.20 cells (Ferrier et al, 1982(Ferrier et al, , 1985 and on ROS 17/2 cells (Dixon et al, 1984), as well as the verapamil and quinine data presented above, provide support for the hypothesis that the membrane potential in these cells is largely controlled by a Ca2 '-activated One pissibility is that the increase in intracellular cells do not..None of the clonal lines shows a change in the spikes and fluctuations as a result of F'TH application. Dibutyryl cAMP produced a hyperpolarization but did not have a significant effect on the spikes and fluctuations.…”
Section: MV Seven Cells)mentioning
confidence: 92%
“…An increase in Ca2+ conductance with a n increase in internal cAMP has been shown to occur in other cell types (Tsien, 1983;Cachelin et al, 1983;Rasmussen and Barrett, 1984). Previous work on RCJ 1.20 cells (Ferrier et al, 1982(Ferrier et al, , 1985 and on ROS 17/2 cells (Dixon et al, 1984), as well as the verapamil and quinine data presented above, provide support for the hypothesis that the membrane potential in these cells is largely controlled by a Ca2 '-activated One pissibility is that the increase in intracellular cells do not..None of the clonal lines shows a change in the spikes and fluctuations as a result of F'TH application. Dibutyryl cAMP produced a hyperpolarization but did not have a significant effect on the spikes and fluctuations.…”
Section: MV Seven Cells)mentioning
confidence: 92%
“…Hypotonic shock has been reported to produce an increase in intracellular calcium levels, initiate a regulatory volume decrease and hyperpolarize osteoblasts (Yamaguchi et al 1989). This hyperpolarization is suggested to be due to an increased K+ conductance mediated by opening of Ca2-sensitive K+ channels known to be present in the plasma membrane of osteoblasts (Dixon, Aubin & Dainty, 1984;Ferrier, Ward-Kesthely, Homble & Ross, 1987;Davison, 1993). To accomplish a decrease in cell volume, this increased K+ permeability is likely to be accompanied by an increased Cl-conductance, leading to KCl efflux and osmotically obligated water.…”
mentioning
confidence: 99%
“…However, hyperpolarization induced by the Ca ππ -selective carboxylic ionophore A23187 has been shown to be effectively antagonized by drugs that interfere with Ca ππ signaling in cells. For example, A23187-induced hyperpolarization can be antagonized by K Ca blockers (Dixon et al 1984), calmodulin antagonists (Henquin 1981;Seeman et al 1983) and Ca ππ channel blockers (Somers et al 1976;Seemann et al 1983). A23187-induced hyperpolarization is believed to arise from an influx of Ca ππ and the resultant stimulation of K Ca currents (Dixon et al 1984;Baumgold & Paek 1992).…”
Section: Discussionmentioning
confidence: 99%
“…For example, A23187-induced hyperpolarization can be antagonized by K Ca blockers (Dixon et al 1984), calmodulin antagonists (Henquin 1981;Seeman et al 1983) and Ca ππ channel blockers (Somers et al 1976;Seemann et al 1983). A23187-induced hyperpolarization is believed to arise from an influx of Ca ππ and the resultant stimulation of K Ca currents (Dixon et al 1984;Baumgold & Paek 1992). Because increased Ca ππ and K Ca currents appear to be involved in hyperpolarization induced by the monovalent cation selective ionophores (see above), drugs influencing Ca ππ signaling in cells were selected as possible antagonists of the membrane hyperpolarization produced by these compounds.…”
Section: Discussionmentioning
confidence: 99%