Electrophysiology of Rodent GnRH Neurons

Piet, Richard; Herbison, Allan E.

doi:10.1002/9781119233275.ch8

Cited by 2 publications

(2 citation statements)

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“…50 This low spontaneous frequency is puzzling because GnRH neuron firing can be driven at much higher rates by current injection, suggesting that the ionic conductances of these cells are not in and of themselves limiting. 23,36 As reviewed previously, 20,21 GnRH neurons recorded in brain slices may be quiescent or spontaneously firing. Although most GnRH neurons exhibit irregular bursting, 1-2% of these cells exhibit parabolic bursting riding on marked slow (approximately 0.05 Hz) oscillations in membrane potential.…”

Section: Gnrh Neuron Firing Patternsmentioning

confidence: 97%

“…For an explanation of recording approaches, the reader is referred to previous reviews. [19][20][21] Initial studies of GnRH neuron properties suffered from a lack of consistent and rigorous methodology that had been established for patch clamp investigation; essentially no laboratory in the field escaped errors, ranging from use of inappropriate pipette solutions, to failure to report quality parameters allowing the reader to evaluate data integrity, as well as failure to explain how properties (e.g., action potential threshold, resting potential) were defined. These limitations resulted in variable data.…”

Section: Intrin S I C Propertie S Of G Nrh Neuronsmentioning

confidence: 99%

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The electrophysiologic properties of gonadotropin‐releasing hormone neurons

Constantin

Moenter

Piet

2021

J Neuroendocrinology

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In the half century since GnRH was sequenced, studies using native GnRH decapeptide and antagonist analogs have demonstrated that the pattern of GnRH release is vital for normal physiology. The hypogonadal (hpg) mouse, a natural GnRH knockout, established this hormone as the critical final output from the central nervous system with respect to regulating the reproductive system through its effects upon the anterior pituitary gonadotropins luteinizing hormone (LH) and follicle-stimulating hormone. Although there is a rich history of physiologic studies concerning the central control of reproduction, we focus here on the neurobiological mechanisms revealed by

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Section: Gnrh Neuron Firing Patternsmentioning

confidence: 97%

Section: Intrin S I C Propertie S Of G Nrh Neuronsmentioning

confidence: 99%

The electrophysiologic properties of gonadotropin‐releasing hormone neurons

Constantin

Moenter

Piet

2021

J Neuroendocrinology

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The Gonadotropin-Releasing Hormone Pulse Generator

2018

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The pulsatile release of GnRH and LH secretion is essential for fertility in all mammals. Pulses of LH occur approximately every hour in follicular-phase females and every 2 to 3 hours in luteal-phase females and males. Many studies over the last 50 years have sought to identify the nature and mechanism of the “GnRH pulse generator” responsible for pulsatile LH release. This review examines the characteristics of pulsatile hormone release and summarizes investigations that have led to our present understanding of the GnRH pulse generator. There is presently little compelling evidence for an intrinsic mechanism of pulse generation involving interactions between GnRH neuron cell bodies. Rather, data support the presence of an extrinsic pulse generator located within the arcuate nucleus, and attention has focused on the kisspeptin neurons and their projections to GnRH neuron dendrons concentrated around the median eminence. Sufficient evidence has been gathered in rodents to conclude that a subpopulation of arcuate kisspeptin neurons is, indeed, the GnRH pulse generator. Findings in other species are generally compatible with this view and suggest that arcuate/infundibular kisspeptin neurons represent the mammalian GnRH pulse generator. With hindsight, it is likely that past arcuate nucleus multiunit activity recordings have been from kisspeptin neurons. Despite advances in identifying the cells forming the pulse generator, almost nothing is known about their mechanisms of synchronicity and the afferent hormonal and transmitter modulation required to establish the normal patterns of LH pulsatility in mammals.

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Electrophysiology of Rodent GnRH Neurons

Cited by 2 publications

References 74 publications

The electrophysiologic properties of gonadotropin‐releasing hormone neurons

The electrophysiologic properties of gonadotropin‐releasing hormone neurons

The Gonadotropin-Releasing Hormone Pulse Generator

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