Electropolymerized Manganese Tetraaminophthalocyanine Thin Films onto Platinum Ultramicroelectrode for the Electrochemical Detection of Peroxynitrite in Solution

Abstract

Peroxynitrite, ONOOÀ also so-called PON, is a very powerful oxidant and cytotoxic agent produced in biological systems by the recombination of nitric oxide and superoxide anion radical. Most of the techniques for assaying PON (chemiluminescence, fluorescence, UV-visible spectroscopy, immunochemistry and EPR ) use indirect methods relying on measurements of secondary species. We report in this study the calibration of a chemically modified Pt ultramicroelectrode by electropolymerized manganese tetraaminophthalocyanine film (MnTAPc) for the determination of PON in aqueous solution. The obtained result allows showing for the first time a real-time calibration curve of the amperometric determination of stable PON in aqueous solution. The sensitivity of the sensor is 14.6 nA mM À1and its detection limit is 5 mM.

Applications of polymerized metal tetra-amino phthalocyanines towards hydrogen peroxide detection

“…In the past two decades, there has been an increase in the use of metallo-phthalocyanine (MPc) complexes as electrode modifiers and their applications for the detection of several analytes [1][2][3][4][5][6][7][8][9][10][11][12][13], due to their excellent electrocatalytic activity. Several methods for modifying electrodes with MPcs have been studied and these include self-assembly [1][2][3][4][5] and electropolymerization [6][7][8][9][10][11][12][13]. Electropolymerization method will be investigated in this work as a method of choice and the polymer-modified electrodes will be studied for their potential as electrochemical sensors for the detection and monitoring of hydrogen peroxide, H 2 O 2 .…”

“…The MPc complexes studied in this work contain cobalt and manganese as metal ion centers and these complexes are known to exhibit excellent electrocatalytic activity [25][26][27][28][29][30]. The amino functionalities on the phthalocyanine macrocycle were chosen due to their known ease of forming polymers on electrode surfaces [12,13,24], which are more stable for analysis compared to SAMs. The electrocatalytic and electroanalytical properties of gold and glassy carbon electrodes modified with thin polymeric films of CoTAPc and MnTAPc are compared for electroreduction of H 2 O 2 .…”

Meat Freshness: Peroxynitrite’s Oxidative Role, Its Natural Scavengers, and New Measuring Tools

“…Bedioui and colleagues reported a number of PON-sensitive films and methods (130)(131)(132), including ONOO − detection on bare gold (Au) microelectrodes (μEs) by amperometry at -0.1 V vs. Ag/AgCl in PBS (132). The simultaneous detection of ONOO − and NO was also revealed.…”

In vivo biosensors

“…These studies and others have led to the need to understand the interplay among a whole host of species whose concentrations change in a linked fashion. These include transport of ions such as Ca 2+ , Na + , K + , H + , and Zn 2+ , as well as oxygen, superoxide [47,48] NO [48–51], ONOO – [52], glucose [53–55], lactate [56], acetylcholine/choline [57,58], and a host of neurotransmitters. This subject has been comprehensively reviewed [59].…”