Electroporation enhances protective immune response of a DNA vaccine against Japanese encephalitis in mice and pigs

Sheng, Ziyang; Gao, Na; Cui, Xiaoyun; Fan, Dongying; Chen, Hui; Wu, Na; Wei, Jianchun; An, Jing

doi:10.1016/j.vaccine.2016.10.001

Cited by 21 publications

(19 citation statements)

References 19 publications

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“…In Australia, a growth hormone-releasing hormone plasmid delivered by EP has been approved in the treatment of pigs (Person et al, 2008). In our recent studies, the DNA vaccine candidates inoculated using EP showed enhanced expression of the antigen in local sites, strong immune response and protective efficacy in mice, rabbits and pigs (Chen et al, 2016; Sheng et al, 2016). Thus, in the present study, we constructed the DNA vaccine candidate pVAX1-D1ME and explored in vivo EP for gene delivery in comparison with traditional IM injection in mice.…”

Section: Discussionmentioning

confidence: 99%

Effective Protection Induced by a Monovalent DNA Vaccine against Dengue Virus (DV) Serotype 1 and a Bivalent DNA Vaccine against DV1 and DV2 in Mice

Zheng

Chen

Wang

et al. 2017

Front. Cell. Infect. Microbiol.

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Dengue virus (DV) is the causal pathogen of dengue fever, which is one of the most rapidly spread mosquito-borne disease worldwide and has become a severe public health problem. Currently, there is no specific treatment for dengue; thus, a vaccine would be an effective countermeasure to reduce the morbidity and mortality. Although, the chimeric Yellow fever dengue tetravalent vaccine has been approved in some countries, it is still necessary to develop safer, more effective, and less costly vaccines. In this study, a DNA vaccine candidate pVAX1-D1ME expressing the prME protein of DV1 was inoculated in BALB/c mice via intramuscular injection or electroporation, and the immunogenicity and protection were evaluated. Compared with traditional intramuscular injection, administration with 50 μg pVAX1-D1ME via electroporation with three immunizations induced persistent humoral and cellular immune responses and effectively protected mice against lethal DV1 challenge. In addition, immunization with a bivalent vaccine consisting of pVAX1-D1ME and pVAX1-D2ME via electroporation generated a balanced IgG response and neutralizing antibodies against DV1 and DV2 and could protect mice from lethal challenge with DV1 and DV2. This study sheds new light on developing a dengue tetravalent DNA vaccine.

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Section: Discussionmentioning

confidence: 99%

Effective Protection Induced by a Monovalent DNA Vaccine against Dengue Virus (DV) Serotype 1 and a Bivalent DNA Vaccine against DV1 and DV2 in Mice

Zheng

Chen

Wang

et al. 2017

Front. Cell. Infect. Microbiol.

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“…JEV Beijing strain-1 (JEV) [7] was propagated in C6/36 cells, and the supernatants were gathered at 3 days post-infection (dpi) and at 4 dpi. The supernatants were allocated and stored at -80 • C for later use.…”

Section: Cells and Virusmentioning

confidence: 99%

Transcriptomic Analysis Suggests the M1 Polarization and Launch of Diverse Programmed Cell Death Pathways in Japanese Encephalitis Virus-Infected Macrophages

Wang

Zhen

Fan

et al. 2020

Viruses

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The Japanese encephalitis virus (JEV) is a Culex mosquito-borne flavivirus and is the pathogenic agent of Japanese encephalitis, which is the most important type of viral encephalitis in the world. Macrophages are a type of pivotal innate immunocyte that serve as sentinels and respond quickly to pathogen invasions. However, some viruses like JEV can hijack macrophages as a refuge for viral replication and immune escape. Despite their crucial involvement in early JEV infection, the transcriptomic landscapes of JEV-infected macrophages are void. Here, by using an in situ JEV infection model, we investigate the transcriptomic alteration of JEV-infected peritoneal macrophages. We found that, upon JEV infection, the macrophages underwent M1 polarization and showed the drastic activation of innate immune and inflammatory pathways. Interestingly, almost all the programmed cell death (PCD) pathways were activated, especially the apoptosis, pyroptosis, and necroptosis pathways, which were verified by the immunofluorescent staining of specific markers. Further transcriptomic analysis and TUNEL staining revealed that JEV infection caused apparent DNA damage. The transcriptomic analysis also revealed that JEV infection promoted ROS and RNS generation and caused oxidative stress, which activated multiple cell death pathways. Our work uncovers the pivotal pathogenic roles of oxidative stress and multiple PCD pathways in JEV infection, providing a novel perspective on JEV–host interactions.

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“…Recently, we have proved that EP could enhance DNA-uptake and expression in mice and pigs [10,11]. Thus, to obtain effective immune responses and protection against DENV2, E and NS1…”

Section: Discussionmentioning

confidence: 99%

Immunization with E Plus NS1-2a Enhanced Protection against Dengue Virus Serotype 2 in Mice

Fang

Cui

et al. 2016

Preprint

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ABSTRACT:Dengue virus (DENV), the causative agent of dengue fever (DF), is one of the most important mosquito-borne viruses that can infect humans. Although much effort has been made on prevention and control of dengue, there are currently no anti-viral drugs or worldwide approved vaccines yet. In this study, we immunized six-week-old Balb/c mice with DNA vaccine candidates E and NS1-2a of DENV serotype 2 or the combination of them (E+NS1-2a) via an electroporation (EP)-assisted intramuscular gene delivery system and evaluated the immune response and protection. The highest specific antibody titres and cytokine levels secreted by splenocytes as well as the highest survival rate were observed in the E+NS1-2a group, followed by E group and NS1-2a group. Our data suggested that the combination of E and NS1-2a delivered by EP may be a superior preventive strategy against DENV.

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Electroporation enhances protective immune response of a DNA vaccine against Japanese encephalitis in mice and pigs

Cited by 21 publications

References 19 publications

Effective Protection Induced by a Monovalent DNA Vaccine against Dengue Virus (DV) Serotype 1 and a Bivalent DNA Vaccine against DV1 and DV2 in Mice

Effective Protection Induced by a Monovalent DNA Vaccine against Dengue Virus (DV) Serotype 1 and a Bivalent DNA Vaccine against DV1 and DV2 in Mice

Transcriptomic Analysis Suggests the M1 Polarization and Launch of Diverse Programmed Cell Death Pathways in Japanese Encephalitis Virus-Infected Macrophages

Immunization with E Plus NS1-2a Enhanced Protection against Dengue Virus Serotype 2 in Mice

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