Electroporation: Parameters affecting transfer of DNA into mammalian cells

Electromobility of plasmid DNA in tumor tissues during electric field-mediated gene delivery

“…5,9,24,25 Although higher strength pulses (eg 500-3000 V/cm), with durations in the microsecond range, have been demonstrated to be useful in transfecting cells in vitro, [26][27][28] Lucas et al 29 demonstrated that low voltage, millisecond duration pulses resulted in a higher gene expression in vivo than high voltage, microsecond duration pulses. A reduction in pulse duration from a millisecond to a microsecond range may cause DNA electrophoresis to be ineffective, as found in our in vitro experiments using 2% agarose gels (data not shown).…”

The latent membrane protein 1 oncogene modifies B-cell physiology by regulating autophagy

“…A total of 5 Â 10 6 cells were electroporated in 500 ml of RPMI 1640 medium supplemented with 10% fetal bovine serum and 50 mM HEPES with a custom-built electroporator at 1500 V, three capacitor banks with 1540 mF of capacitance, R-adjust set at 0600 and a rise time set at 1000 as described previously (Knutson and Yee, 1987).…”

Efficient expression of foreign genes in human CD34+ hematopoietic precursor cells using electroporation

“…While this may depend on cell type, a previous report demonstrated similar electroporation efficiencies for two human cell lines for concentrations between 1 × 10 6 and 20 × 10 6 cells/ml. 22 In cases where the quantity of CD34 + cells is limited, valid data can be obtained with relatively low cell concentrations. In contrast, when there is a need to electroporate large numbers of cells, such as in clinical applications, great savings on time and supply resources would be possible by using a higher concentration of cells.…”