Electroporation Therapy of Skin Cancer in the Head and Neck Area

Landström, Fredrik; Nilsson, Christer; Crafoord, Sven; Reizenstein, Johan; Adamsson, Gun-Britt; Löfgren, Lennart

doi:10.1111/j.1524-4725.2010.01617.x

Cited by 57 publications

(33 citation statements)

References 21 publications

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“…Electric pulses can also cause irreversible membrane breakdown resulting in cell death, termed irreversible electroporation (IE) [8], [9], [10], [11], [12], [13]. Killing of unwanted cells and tissue by IE and electrochemotherapy has shown to be effective in the treatment of cancers [4], [6], [9], [11], [12], [14]. The use of ultrashort electric pulses (USEP) has emerged as a novel modality to kill cells based on theoretical and empirical results showing that USEP can cause intracellular membrane poration.…”

Section: Introductionmentioning

confidence: 99%

Dose-Dependent Thresholds of 10-ns Electric Pulse Induced Plasma Membrane Disruption and Cytotoxicity in Multiple Cell Lines

Ibey¹,

Roth

Pakhomov

et al. 2011

PLoS ONE

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In this study, we determined the LD50 (50% lethal dose) for cell death, and the ED50 (50% of cell population staining positive) for propidium (Pr) iodide uptake, and phosphatidylserine (PS) externalization for several commonly studied cell lines (HeLa, Jurkat, U937, CHO-K1, and GH3) exposed to 10-ns electric pulses (EP). We found that the LD50 varied substantially across the cell lines studied, increasing from 51 J/g for Jurkat to 1861 J/g for HeLa. PS externalized at doses equal or lower than that required for death in all cell lines ranging from 51 J/g in Jurkat, to 199 J/g in CHO-K1. Pr uptake occurred at doses lower than required for death in three of the cell lines: 656 J/g for CHO-K1, 634 J/g for HeLa, and 142 J/g for GH3. Both Jurkat and U937 had a LD50 lower than the ED50 for Pr uptake at 780 J/g and 1274 J/g, respectively. The mechanism responsible for these differences was explored by evaluating cell size, calcium concentration in the exposure medium, and effect of trypsin treatment prior to exposure. None of the studied parameters correlated with the observed results suggesting that cellular susceptibility to injury and death by 10-ns EP was largely determined by cell physiology. In contrast to previous studies, our findings suggest that permeabilization of internal membranes may not necessarily be responsible for cell death by 10-ns EP. Additionally, a mixture of Jurkat and HeLa cells was exposed to 10-ns EP at a dose of 280 J/g. Death was observed only in Jurkat cells suggesting that 10-ns EP may selectively kill cells within a heterogeneous tissue.

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Section: Introductionmentioning

confidence: 99%

Dose-Dependent Thresholds of 10-ns Electric Pulse Induced Plasma Membrane Disruption and Cytotoxicity in Multiple Cell Lines

Ibey¹,

Roth

Pakhomov

et al. 2011

PLoS ONE

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“…To reduce movement, muscle relaxants may be administered to patients prior to treatment [10,[13][14][15]. This poses additional concerns for the anesthesiologist, as the dosage of muscle relaxants must be continually monitored to ensure an adequate neuromuscular blockade and proper respiratory function [16].…”

Section: Introductionmentioning

confidence: 99%

Advances in Therapeutic Electroporation to Mitigate Muscle Contractions

Arena¹,

Davalos²

2012

J Membra Sci Technol

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“…Additionally subsequent studies 11,49,66,[89][90][91][92] of ECT evaluating its effect in the treatment of melanoma and other skin cancers continue to demonstrate the efficacy of the treatment, with response rates comparable to the earlier studies, ranging from 46-100%. Repeated treatments are feasible as demonstrated by Campana et al 90 and Quaglino et al 11 producing additional clinical responses in patients who had initial non or partial responses or who presented with new recurrent lesions.…”

Section: Wwwintechopencommentioning

confidence: 71%