Electrospinning Drug-Loaded Alginate-Based Nanofibers towards Developing a Drug Release Rate Catalog

Penton, Kathryn E.; Kinler, Zachary; Davis, Amber; Spiva, Joshua A.; Hamilton, Sharon K.

doi:10.3390/polym14142773

Cited by 9 publications

(4 citation statements)

References 38 publications

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“…The elution solution was made in the amount of 85% acetonitrile and 15% water. HPLC was taken from the sample at 263 nm and various pH and times up to 48 h 43,44 .…”

Section: Methodsmentioning

confidence: 99%

Architecture of a dual biocompatible platform to immobilize genistin: Fabrication with physio-chemical and in vitro evaluation

Esmaeilneia,

Dehkharghani,

Benisi

2023

Preprint

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In this research, genistin was obtained by extracting from soybean meal powder, and then by adding polycaprolactone (PCL), genistin nanocapsules were prepared. For the first time, we used a lipophilic nanophase (encapsulated genistin) coated in hydrophilic nanofibers (gelatin /polyvinyl alcohol) as a dual nanosystem by the electrospinning method. In the approach, the nanofibers mimic the natural extracellular matrix, interact favorably with cells being cultured from one side, and raise the local concentration of a bioactive compound at the cell surface. The encapsulated drug was loaded in fibers with various percentages, which showed appropriate and significant controlled release using high-performance liquid chromatography (HPLC). To prove the experiments, analysis using an ultraviolet-visible spectrometer (UV-Vis), 1 H NMR spectrometer, Fourier transforms infrared spectrometer (FTIR), mechanical test, scanning electron microscope (SEM) and microscope transmission electron microscopy (TEM) was performed. Finally, excellent biological effects, viability, and non-toxicity of the sample synthesized by the MTT method and significant proliferation and adhesion on mouse fibroblast cell line L929 were obtained.

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“…The elution solution was made in the amount of 85% acetonitrile and 15% water. HPLC was taken from the sample at 263 nm and various pH and times up to 48 h 43,44 .…”

Section: Methodsmentioning

confidence: 99%

Architecture of a dual biocompatible platform to immobilize genistin: Fabrication with physio-chemical and in vitro evaluation

Esmaeilneia,

Dehkharghani,

Benisi

2023

Preprint

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show abstract

“…Utilizing high-performance liquid chromatography (HPLC), the sample was subjected to analysis at a wavelength of 263 nm using an eluent composed of 85% acetonitrile and 15% water. The analysis was conducted at different pH levels and for varying durations, with a maximum timeframe of 48 h 47 , 48 .…”

Section: Methodsmentioning

confidence: 99%

Architecture of a dual biocompatible platform to immobilize genistin: fabrication with physio-chemical and in vitro evaluation

Esmaeilneia,

Amiri Dehkharghani,

Zamanlui Benisi

2023

Sci Rep

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The design of biocompatible cell culture substrates and electrospun nanofibers can improve cell proliferation and behavior in laboratory conditions for tissue engineering applications in medicine. In this research, genistin was obtained by extracting from soybean meal powder, and then by adding polycaprolactone (PCL), genistin nanocapsules were prepared. For the first time, we used a lipophilic nanophase (encapsulated genistin) coated in a hydrophilic nanophase (gelatin /polyvinyl alcohol) as a dual nanosystem by the electrospinning method. In the approach, the nanofibers mimic the natural extracellular matrix, interact favorably with cells being cultured from one side, and raise the local concentration of a bioactive compound at the cell surface. The encapsulated drug which was inserted in fibers with a loading percentage of 92.01% showed appropriate and significant controlled release using high-performance liquid chromatography (HPLC). To prove the experiments, analysis using an ultraviolet–visible spectrometer (UV–Vis), 1H NMR spectrometer, Fourier transforms infrared spectrometer (FTIR), mechanical test, scanning electron microscope (SEM) and microscope transmission electron microscopy (TEM) was performed. The sample synthesized with 40% drug using the MTT method exhibited remarkable biological effects, viability, and non-toxicity. Additionally, significant proliferation and adhesion on the mouse fibroblast cell line L929 were observed within a 72-h timeframe.

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“…Calcium ions in the alginate interact with sodium ions in the wound exudate, causing the alginate to form a gel at the site of the wound, providing an appropriately moist environment for wound healing [ 126 ]. However, due to the low molecular weight of sodium alginate, it tends to form a gel at slightly higher concentrations, making it difficult to synthesize pure sodium alginate nanofiber materials [ 127 ].…”

Section: Nanofiber Scaffold Technologymentioning

confidence: 99%

Nanofiber Scaffolds as Drug Delivery Systems Promoting Wound Healing

Jiang

Zheng

et al. 2023

Pharmaceutics

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Nanofiber scaffolds have emerged as a revolutionary drug delivery platform for promoting wound healing, due to their unique properties, including high surface area, interconnected porosity, excellent breathability, and moisture absorption, as well as their spatial structure which mimics the extracellular matrix. However, the use of nanofibers to achieve controlled drug loading and release still presents many challenges, with ongoing research still exploring how to load drugs onto nanofiber scaffolds without loss of activity and how to control their release in a specific spatiotemporal manner. This comprehensive study systematically reviews the applications and recent advances related to drug-laden nanofiber scaffolds for skin-wound management. First, we introduce commonly used methods for nanofiber preparation, including electrostatic spinning, sol–gel, molecular self-assembly, thermally induced phase separation, and 3D-printing techniques. Next, we summarize the polymers used in the preparation of nanofibers and drug delivery methods utilizing nanofiber scaffolds. We then review the application of drug-loaded nanofiber scaffolds for wound healing, considering the different stages of wound healing in which the drug acts. Finally, we briefly describe stimulus-responsive drug delivery schemes for nanofiber scaffolds, as well as other exciting drug delivery systems.

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Electrospinning Drug-Loaded Alginate-Based Nanofibers towards Developing a Drug Release Rate Catalog

Cited by 9 publications

References 38 publications

Architecture of a dual biocompatible platform to immobilize genistin: Fabrication with physio-chemical and in vitro evaluation

Architecture of a dual biocompatible platform to immobilize genistin: Fabrication with physio-chemical and in vitro evaluation

Architecture of a dual biocompatible platform to immobilize genistin: fabrication with physio-chemical and in vitro evaluation

Nanofiber Scaffolds as Drug Delivery Systems Promoting Wound Healing

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