Electrospinning of nanofibres with parallel line surface texture for improvement of nerve cell growth

Ouyang

ACS Appl. Mater. Interfaces

et al. 2015

Self Cite

130

A novel fibrous conduit consisting of well-aligned nanofibers with longitudinal nanogrooves on the fiber surface was prepared by electrospinning and was subjected to an in vivo nerve regeneration study on rats using a sciatic nerve injury model. For comparison, a fibrous conduit having a similar fiber alignment structure without surface groove and an autograft were also conducted in the same test. The electrophysiological, walking track, gastrocnemius muscle, triple-immunofluorescence, and immunohistological analyses indicated that grooved fibers effectively improved sciatic nerve regeneration. This is mainly attributed to the highly ordered secondary structure formed by surface grooves and an increase in the specific surface area. Fibrous conduits made of longitudinally aligned nanofibers with longitudinal nanogrooves on the fiber surface may offer a new nerve guidance conduit for peripheral nerve repair and regeneration.

Section: Introductionmentioning

confidence: 99%

Nerve Guidance Conduits from Aligned Nanofibers: Improvement of Nerve Regeneration through Longitudinal Nanogrooves on a Fiber Surface

Ouyang

ACS Appl. Mater. Interfaces

et al. 2015

Self Cite

130

“…The disc‐electrospun nanofibers looked uniform in fiber morphology and contained no beads, which were similar to those electrospun from the needle electrospinning. At the concentration of 12%, CAB was electrospun into smooth fibers without a parallel‐grooved surface feature 26. The needle‐electrospun nanofibers had an average diameter of 367 nm.…”

Section: Resultsmentioning

confidence: 99%

“…Fibroblasts were selected as they can be easily regulated by the contact guidance from culture substrates 27. Our previous study has found that Schwann cells exhibiting prompt proliferation on electrospun CAB nanofibers and the growth behavior could be readily regulated though the control of fiber orientation 26…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Disc‐electrospun cellulose acetate butyrate nanofibers show enhanced cellular growth performances

J Biomedical Materials Res

et al. 2012

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Cellulose acetate butyrate nanofibers were prepared separately by two electrospinning techniques; a needleless electrospinning using a disc as spinneret and a rotary drum as collector and a conventional needle electrospinning using a rotary drum as collector. Compared to the needle-electrospun nanofibers, the disc-electrospun nanofibers were coarser with a wider diameter distribution. Both fibers had a similar surface morphology and they showed no difference in chemical components, but the disc-electrospun nanofibers were slightly higher in crystallinity. The productivity of disc electrospinning was 150 times larger than that of needle electrospinning. The disc-electrospun nanofiber mats were found to have a three dimensional fibrous structure with an average pore size of 9.1 μm, while the needle-electrospun nanofibers looked more like a two-dimensional sheet with a much smaller average pore size (3.2 μm). Fibroblasts and Schwann cells were cultured on the fibrous matrices to assess the biocompatibility. The disc-electrospun nanofiber webs showed enhanced cellular growth for both fibroblasts and Schwann cells, especially in a long culture period.

“…Depending on the spinneret structure, collecting mode and operating parameters, they can have aligned fibrous structures [6], bicomponent cross-sectional configurations [7,8], ribbon shapes [9] or porous surfaces [10]. In our previous study, we have found that nanofibers with a parallel line surface texture can be electrospun from a polymer solution when a mixed solvent system consisting of a highly volatile solvent (e.g., acetone) and a high boiling point organic solvent are employed [11]. The highly-ordered surface texture on the fibers was found to be beneficial to the growth of nerve cells regardless of the fiber orientation.…”

Section: Introductionmentioning

confidence: 99%

Needleless Electrospinning of Polystyrene Fibers with an Oriented Surface Line Texture

Journal of Nanomaterials

et al. 2012

Self Cite

We have demonstrated that polystyrene (PS) nanofibers having an ordered surface line texture can be produced on a large scale from a PS solution of acetone and N,N′-dimethylformamide (2/1, vol/vol) by a needleless electrospinning technique using a disc as fiber generator. The formation of the unusual surface feature was investigated and attributed to the voids formed on the surface of jets due to the fast evaporation of acetone at the early stage of electrospinning, and subsequent elongation and solidification turning the voids into ordered lines on fiber surface. In comparison with the nanofibers electrospun by a conventional needle electrospinning using the same solution, the disc electrospun fibers were finer with similar diameter distribution. The fiber production rate for the disc electrospinning was 62 times higher than that of the conventional electrospinning. Fourier transform infrared spectroscopy and X-ray diffraction measurements indicated that the PS nanofibers produced from the two electrospinning techniques showed no significant difference in chemical component, albeit slightly higher crystallinity in the disc spun nanofibers.