Electrospinning of PVA/chitosan nanocomposite nanofibers containing gelatin nanoparticles as a dual drug delivery system

Abstract

Nanofibrous core-sheath nanocomposite dual drug delivery system based on poly(vinyl alcohol) (PVA)/chitosan/lidocaine hydrochloride loaded with gelatin nanoparticles were successfully prepared by the electrospinning method. Gelatin nanoparticles were prepared by nanoprecipitation and were then loaded with erythromycin antibiotic agent with the average particle size of ∼175 nm. The morphology of gelatin nanoparticles observed by field emission scanning electron microscopy (FE-SEM) was shown to be optimal at the concentration of 1.25 wt % of gelatin in aqueous phase by addition of 20 µL of glutaraldehyde 5% as the crosslinking agent. The nanoparticles were also characterized by dynamic light scattering, zeta potential measurement, and Fourier transform infrared spectroscopy (FTIR). The best bead free morphology for the PVA/chitosan nanofibrous mats were obtained at the solution weight ratio of 96/4. The nanofibrous mats were analyzed by swelling studies, FTIR and antibacterial tests. In vitro dual release profile of the core-sheath nanofibers was also studied within 72 h and showed the release efficiency equal to 84.69 and 75.13% for lidocaine hydrochloride and erythromycin, respectively. According to release exponent n, the release of lidocaine hydrochloride from the sheath part of the matrix is quasi-Fickian diffusion mechanism, while the release of erythromycin is based on anomalous or non-Fickian mechanisms.

Preparation of chitosan–silica/PCL composite membrane as wound dressing with enhanced cell attachment

“…Highly porous bottom sides of PCL membranes faced the receptor compartment. Pseudo‐extracellular fluid was the release medium with the pH of 8.00 ± 0.5 stirred at 100 rpm. To detect the amount of lidocaine HCl, the absorbance of the sample solution was measured by UV–Vis spectrophotometer at maximum λ (263 nm) in fixed intervals .…”

Electrospinning and crosslinking of polyvinyl alcohol/chitosan composite nanofiber for transdermal drug delivery

“…[14][15][16][17] Furthermore, CS is reported to have pharmacological properties including wound healing accelerator, [18] hyphcholesterolemic action, [19] antacid and antiulcer activity. [24] However, CS is hardly electrospinnable because CS acidic solution is a cationic polyelectrolyte. [24] However, CS is hardly electrospinnable because CS acidic solution is a cationic polyelectrolyte.…”

“…[24] However, CS is hardly electrospinnable because CS acidic solution is a cationic polyelectrolyte. Fathollahipour et al [24] have fabricated electrospun PVA/CS composite nanofibers containing gelation nanoparticles as a dual drug delivery system. [25] To overcome the difficulties in electrospinning of CS, CS blended with a easily electrospinnable polymer, for example PVA, [26] poly(ethylene oxide), [27] gelatin, [28] silk fibroin, [29] and so forth were investigated by researchers.…”

Zein nanoparticle‐embedded electrospun PVA nanofibers as wound dressing for topical delivery of anti‐inflammatory diclofenac

“…The carrier nanosystem is determined by the chemical and physical properties of molecules loaded into the NPs . Therefore, various kinds of materials have been used for fabrication of these nanostructures . Among them, polymeric drug‐delivery systems have great utility because of their superior properties, such as improved efficiency, minimized toxicity, and enhanced bioavailability of the drug .…”

“…3 Therefore, various kinds of materials have been used for fabrication of these nanostructures. 4 Among them, polymeric drug-delivery systems have great utility because of their superior properties, such as improved efficiency, minimized toxicity, and enhanced bioavailability of the drug. 5,6 Polymeric NPs such as chitosan, 7 poly(lactic-co-glycolic acid), 8 and gelatin NPs 4 have been reported as nanocarriers to deliver different drugs and biologically active molecules for wound healing application.…”