Electrospinning of silk fibroin from all aqueous solution at low concentration

Kishimoto, Yuki; Morikawa, Hideaki; Yamanaka, Shigeru; Tamada, Yasushi

doi:10.1016/j.msec.2016.12.113

Cited by 66 publications

(54 citation statements)

References 36 publications

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“…Furthermore, the aqueous solvent system could minimize safety problems that may occur when using organic solvents. 41 Secondly, the FG electrospinning can carry caffeine in an amount close to the maximum solubility in water while preventing crystallization. Third, FG electrospinning can produce nanobers with the thinnest diameter of 200 nm on average.…”

Section: Discussionmentioning

confidence: 99%

Fish gelatin nanofibers prevent drug crystallization and enable ultrafast delivery

et al. 2017

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For drug delivery, uncontrolled drug crystallization is the most important and well-described problem which affects the compatibility between target drug and carrier polymer matrix. The crystallization of drugs makes a drug delivery system unstable and it causes a drop the drug loading efficiency and unpredictable release behavior. Recently, the electrospinning process which has rapid solvent evaporation speed has been proposed to prevent the drug crystallization during the drug loading process. In the present study, a fastdissolving nanofibrous drug delivery carrier was prepared via electrospinning of aqueous fish gelatin as the polymer carrier and caffeine as the hydrophilic model drug under environmentally friendly conditions. Fish gelatin electrospinning was able to produce caffeine loaded gelatin nanofibers with a diameter of 200-220 nm. The X-ray diffraction (XRD) and differential scanning calorimetry (DSC) data clearly showed that the amorphous state of caffeine was well incorporated into ultrafine nanofibrous carrier whereas crystalline caffeine was dispersed into conventional fish gelatin film. Fish gelatin nanofibers were able to load two times more caffeine (20 mg ml À1) than conventional caffeine-loaded polymer nanofibers. The fish gelatin nanofibrous mat has good flexibility compared to film due to its interporous nanofiber network. The fish gelatin nanofibrous mats disintegrated within 1.5 s, 27 times faster than films. Fish gelatin nanofibers also showed that most caffeine was released within 10 seconds, which is faster than fish gelatin film and free caffeine drugs. These fish gelatin nanofibers showed 10 times faster caffeine release rates than other caffeine loaded polymer nanofibers. Electrospun fish gelatin nanofibrous mats can be used in biomedical fields especially for the ultra fast delivery of hydrophilic drugs or active ingredients.

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Section: Discussionmentioning

confidence: 99%

Fish gelatin nanofibers prevent drug crystallization and enable ultrafast delivery

et al. 2017

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“…Along these lines, hydrated silk is an attractive biomedical material due to its biocompatibility, stability, and strength . The ability to generate silk materials in a variety of architectures through artificial spinning, drop casting, electrospinning, dip coating, foam formation, and emulsification is also advantageous. Several comprehensive reviews have been published regarding the application of silk as a biomedical material .…”

Section: Silk As a Supramolecular Nanomaterialsmentioning

confidence: 99%

Silk and Silk‐Like Supramolecular Materials

Fink

Zha

2018

Macromol. Rapid Commun.

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Silk is a source of marvel for centuries as one of nature's high-performance materials. More recently, chemical and structural analysis techniques have helped explore the relationship between silk's properties and its hierarchical structure. Furthermore, recombinant protein engineering as well as polymer and organic synthesis techniques have enabled the production of silk-like materials. It has become apparent that silk is a supramolecular polymer with many of the properties exhibited by well-known synthetic supramolecular materials, such as block copolymers, liquid crystals, thermoplastic elastomers, and self-assembling peptides. In this review, the hierarchical structure and supramolecular assembly of silk are discussed in comparison to these synthetic supramolecular systems. By focusing on the connections between chemical structure, nanoscale molecular organization, and material properties, the aim is to provide perspectives on the rational design of advanced soft matter to supramolecular chemists and molecular engineers who look to nature for inspiration.

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“…14,21,24 Widely used natural polymers for wound healing include chitosan, 1,16 collagen, 25,26 alginate, 27 gelatin, 28 chitin, 29 and silk fibroin. 30 However, wound dressings prepared from natural polymers are unable to retain their structural stability, 31 while those from synthetic polymers usually have good mechanical properties. 14,21 Interestingly, a nanofibrous scaffold achieved by combining natural and synthetic polymers is expected to exhibit physicochemical properties of both components.…”

Section: Introductionmentioning

confidence: 99%

Electrospun PCL/mupirocin and chitosan/lidocaine hydrochloride multifunctional double layer nanofibrous scaffolds for wound dressing applications

Li¹,

Wang²,

Yang³

et al. 2018

IJN

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BackgroundAn ideal wound dressing should exhibit good biocompatibility, minimize pain and infection, absorb excess exudates, and maintain a moist environment. However, few clinical products meet all these needs. Therefore, the aim of this study was to fabricate a multifunctional double layer nanofibrous scaffolds (DLS) as a potential material for wound dressing.Materials and methodsThe scaffold was formed from mupirocin and lidocaine hydrochloride homogeneously incorporated into polycaprolactone as the first layer of scaffolds and chitosan as the second layer of scaffolds nanofibers through electrospinning. The fabricated nanofibrous scaffolds were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, differential scanning calorimetry, and measurement of swelling ratio, contact angle, drug release, and mechanical properties. Furthermore, antibacterial assessment, live/dead cell assays, and MTT assays were used to investigate the antibacterial activity and cytotoxicity of the nanofibrous scaffolds.ResultsThe morphology of the nanofibrous scaffolds was studied by scanning electron microscopy, showing successful nanofibrous scaffolds. Fourier transform infrared spectroscopy demonstrated the successful incorporation of the material used to produce the produced nanofibrous scaffolds. Thermal studies with thermogravimetric analysis and differential scanning calorimetry indicated that the DLS had high thermal stability. The DLS also showed good in vitro characteristics in terms of improved swelling ratio and contact angle. The mechanical results revealed that the DLS had an improved tensile strength of 3.88 MPa compared with the second layer of scaffold (2.81 MPa). The release of drugs from the scaffold showed different profiles for the two drugs. Lidocaine hydrochlo ride exhibited an initial burst release (66% release within an hour); however, mupirocin exhibited only a 5% release. Furthermore, the DLS nanofibers displayed highly effective antibacterial activities against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa and were nontoxic to fibroblasts.ConclusionThe fabricated DLS exhibited excellent hydrophilicity, cytocompatibility, sustained drug release, and antibacterial activity, which are favorable qualities for its use as a multifunctional material for wound dressing applications.

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Electrospinning of silk fibroin from all aqueous solution at low concentration

Cited by 66 publications

References 36 publications

Fish gelatin nanofibers prevent drug crystallization and enable ultrafast delivery

Fish gelatin nanofibers prevent drug crystallization and enable ultrafast delivery

Silk and Silk‐Like Supramolecular Materials

Electrospun PCL/mupirocin and chitosan/lidocaine hydrochloride multifunctional double layer nanofibrous scaffolds for wound dressing applications

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