Electrospray Ionization and Time-of-Flight Mass Spectrometric Method for Simultaneous Determination of Spermidine and Spermine

Samejima, Keijiro; Ôtani, Midori; Murakami, Yasuko; Oka, Takami; Kasai, Misao; Tsumoto, Hiroki; Kohda, Kohfuku

doi:10.1248/bpb.30.1943

Cited by 13 publications

(6 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the derivative procedure was selected since polyamines do not contain a suitable chromophore or fluorophore group and possess the properties of low volatility and low molecular weight. Therefore, derivatization using a suitable labeling reagent is a key step in GC‐MS (Chen et al ., ; Paik et al ., ) and LC‐MS (Byun et al ., ; Liu et al ., ; Moriya et al ., ; Samejima et al ., , ) analyses of polyamines. There is a significant number of precolumn labeling methods using derivatization reagents, for example, isobutyl chloroformate (Byun et al ., ), dansyl chloride (Ducros et al ., ), benzoyl chloride (Liu et al ., ; Asotra et al ., ), N ‐ethoxycarbonyl‐ N ‐pentafluoropropionyl (Paik et al ., ) and N ‐heptafluorobutyryl (Li et al ., ) for the analysis of the polyamines.…”

Section: Introductionmentioning

confidence: 99%

A quantitative analysis of the polyamine in lung cancer patient fingernails by LC‐ESI‐MS/MS

Min

Matsumoto

Gao

et al. 2013

Biomedical Chromatography

View full text Add to dashboard Cite

A quantitative analysis of polyamines in lung cancer patient fingernails by the combination of 4-(N,N-dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole derivatives and liquid chromatography-electrospray ionization tandem mass spectrometry is described. The reaction of the reagent with eight kinds of polyamines, that is, N(1) -acetylputrescine (N(1) -actPUT), N(8) -acetylspermidine, N(1) -acetylspermine, 1,3-diaminopropane, putrescine (PUT), cadaverine, spermidine and spermine (SPM) effectively occurs at 60 °C for 30 min. The detection limits (signal-to-noise ratio 5) were 5-100 fmol. A good linearity was achieved from the calibration curves, which was obtained by plotting the peak area ratios of the analytes relative to the internal standard (IS), that is, 1,6-diaminohexane, vs the injected amounts of polyamines (r(2) > 0.996), and the intra-day and inter-day assay precisions were <9.84%. Furthermore, the recoveries (%) of the polyamines spiked in the human fingernails were 89.14-110.64. The present method was applied to human fingernail samples from 17 lung cancer patients and 39 healthy volunteers. The polyamine concentration was different based on the gender, that is, the N(1) -actPUT and PUT contents were 3.10 times and 2.56 times higher in healthy men than in women, respectively. Additionally, in the lung cancer patient group, as compared with the healthy volunteers, the concentrations of SPM had a statistically significant (p < 0.05) correlation. Therefore, because the proposed method provides a good mass accuracy and the trace detection of the polyamines in human fingernails, this analytical technique could be a noninvasive technique to assist in the diagnosis and assessment of disease activity in lung cancer patients.

show abstract

Section: Introductionmentioning

confidence: 99%

A quantitative analysis of the polyamine in lung cancer patient fingernails by LC‐ESI‐MS/MS

Min

Matsumoto

Gao

et al. 2013

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…A time-of-flight mass spectrometer with electrospray ionization interface method to determine the concentrations of spermidine and spermine in mammalian cells was described by Samejima et al (72). Data acquisition of one sample required approximately 2 minutes.…”

Section: Downloaded By [University Of Windsor] At 17:54 20 November 2014mentioning

confidence: 99%

Current Status of Polyamine and Polyamine Analogs Analysis in Cancer Research

Önal

2010

Critical Reviews in Analytical Chemistry

View full text Add to dashboard Cite

The amino-acid-derived polyamines have been associated with cell growth and cancer and their concentrations in malignant tumors are extremely high compared to those in tissues that are histologically normal. Hence, polyamines have been considered as an interesting object for anticancer therapy. Analysis of polyamine levels in biological fluid can possibly provide helpful information on the types of cancer and progression phase of the disease. Numerous modern analytical methods, including high-performance liquid chromatography, gas chromatography, capillary electrophoresis, and other separation techniques have been widely utilized for analysing polyamine levels. In both tissue culture and experimental animal models, polyamine analogues restrain cell growth and destroy malignant cells. Determination of intracellular analogue contents is also crucial, since analogue accumulation is a causal factor for their antitumour effectiveness. In this review, the latest methods reported for polyamines and polyamine analogs in cancer research are discussed.

show abstract

“…The cell extracts were centrifuged (12 000 g, 10 min, 4 8C), and the supernatant was used for the measurement of putrescine, spermidine and spermine contents. The amount of putrescine, spermidine and spermine was quantitated by mass spectrometric analysis (Samejima et al 2007). …”

Section: Measurement Of Cellular Polyaminesmentioning

confidence: 99%

Antizyme is necessary for conversion of pancreatic tumor cells into glucagon-producing differentiated cells

Suzuki¹,

Murakami²,

Samejima³

et al. 2009

Endocrine-Related Cancer

Self Cite

View full text Add to dashboard Cite

Human pancreatic tumor cell lines -AsPC-1, PANC-1, MIA paca2, KP-1 and KP-59 cellscan be induced to differentiate into pancreatic hormone-producing cells by brief trypsin treatment and subsequent culture in a serum-free, chemically defined medium. During culture, AsPC-1 cells formed cell clusters resembling the pancreatic islets, expressed genes associated with the pancreatic development and produced glucagon but not insulin. When PANC-1, MIA paca2, KP-1 and KP-59 cells were treated and cultured the same way, they underwent similar morphological changes and produced insulin and glucagon. We used these systems to identify intracellular regulatory molecules involved in the conversion of pancreatic tumor cells into glucagon-producing cells. We found that the expression of antizyme 1 (AZ1), a negative regulator of ornithine decarboxylase, was increased and its localization was altered from the nucleus to the cytoplasm during AsPC-1 cell differentiation. Transient transfection of AsPC-1 cells with AZ1 siRNA resulted in inhibition of the morphological and functional cell differentiation as well as the specific suppression of AZ1 expression. By contrast, constitutive overexpression of AZ1 in AsPC-1 cells led to the enhancement of glucagon production. We also found that PANC-1 cells reduced the expression of glucagon mRNA when treated with AZ1 siRNA. These results suggested that AZ1 was necessary for the conversion of pancreatic tumor cells into glucagon-producing cells. Glucagon production in AsPC-1 cells was not affected by addition of putrescine, suggesting that the polyamines were not directly involved in the AZ1-mediated conversion of pancreatic tumor cells to differentiated state.

show abstract

Electrospray Ionization and Time-of-Flight Mass Spectrometric Method for Simultaneous Determination of Spermidine and Spermine

Cited by 13 publications

References 15 publications

A quantitative analysis of the polyamine in lung cancer patient fingernails by LC‐ESI‐MS/MS

A quantitative analysis of the polyamine in lung cancer patient fingernails by LC‐ESI‐MS/MS

Current Status of Polyamine and Polyamine Analogs Analysis in Cancer Research

Antizyme is necessary for conversion of pancreatic tumor cells into glucagon-producing differentiated cells

Contact Info

Product

Resources

About