Electrospray Ionization Mass Spectrometric Analysis of Blood for Differentiation of Species

Mass spectrometry-based proteomics as a tool to identify biological matrices in forensic science

“…Additionally, hemoglobin is not only very specific and highly abundant in blood but it also allows to distinguish between different species of origin. Espinoza et al found that hemoglobin, analyzed by electrospray ionization mass spectrometry, can resolve species by the presence of species determining peptides [24]. Despite extensive homology, all three species tested ( Homo sapiens , Canis familiaris , and Bos taurus ) were unambiguously annotated based on highly confident identification of species-specific peptide stretches.…”

A brief overview of forensic herpetology

“…In cases where traditional field identification guides and dichotomous keys prove inadequate, new methods must be developed for identifying such remains. In addition to traditional morphological techniques (Brazaitis, 1986;Busack and Pandya, 2001;Fuchs & Fuchs, 2003), such methods increasingly include genetics (see McDowall, this series) and analytical chemistry (Espinoza et al, 1996(Espinoza et al, , 1999Espinoza et al, 2007).…”

Using species-specific repeat and PCR–RFLP in typing of DNA derived from blood of human and animal species

“…The conventional molecular methods for species identification have been mainly based on protein markers, immunological testing, electrophoresis, high-performance liquid chromatography analysis and DNA hybridization [5][6][7][8][9][10][11]. Application of such protocols has, however, failed to successfully differentiate closely related species, highlighting the need for a method with higher specificity and sensitivity.…”